Evaluating the Toxicity of Hydroxyapatite Nanoparticles in Catfish Cells and Zebrafish Embryos

Zhao, Xinxin; Ong, Kimberly J.; Ede, James D.; Stafford, James L.; Ng, Kee Woei; Goss, Greg G.; Loo, Say Chye Joachim

doi:10.1002/smll.201200639

Cited by 48 publications

(29 citation statements)

References 52 publications

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“…In addition, Pal et al (2007) found that truncated triangular Ag NPs inhibited bacteria (E. coli) more effectively than Ag nano-spheres or nano-rods. Furthermore, needleshaped hydroxyapatite was found to delay embryo hatching in zebrafish more than rod-shaped hydroxyapatite after 72 h (Zhao et al, 2012). Differences in toxicity among ENPs with different shapes might be a result of differences in bioaccumulation, differences in reactivity, or both, and teasing apart these differences is necessary for sound risk assessments.…”

Section: Introductionmentioning

confidence: 98%

Influence of copper oxide nanoparticle form and shape on toxicity and bioaccumulation in the deposit feeder, Capitella teleta

Dai

Banta

Selck

et al. 2015

Marine Environmental Research

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Section: Introductionmentioning

confidence: 98%

Influence of copper oxide nanoparticle form and shape on toxicity and bioaccumulation in the deposit feeder, Capitella teleta

Dai

Banta

Selck

et al. 2015

Marine Environmental Research

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“…When exposed to the embryos, the dot particles may translocate through the chorionic pores of the zebrafish and get trapped within the chorionic space. NPs trapped in the chorion can restrict the diffusion of nutrients and oxygen supply to the embryo, causing axial deformities in the tail and spine as well as edema [25,26,27]. The accumulated nanoparticles can also alter the charge, diffusion, and interactions of chorionic proteins such as the hatching enzyme and membrane transporter, leading to interference in the signaling cascades of embryonic development [25,28].…”

Section: Resultsmentioning

confidence: 99%

The Influence of Hydroxyapatite Nanoparticle Morphology on Embryonic Development in a Zebrafish Exposure Model

Pujari-Palmer

Ott

2017

Nanomaterials

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Nanomaterials are used in many different industries such as cosmetics, food, clothing, and electronics. There is increasing concern that exposure to nanoparticles (NPs) during pregnancy can adversely affect fetal development. It is well known that the size, charge, and chemistry of a nanoparticle can modulate embryological development. The role that particle morphology plays on early development, however, is still widely unknown. The present study aims to investigate the effect of hydroxyapatite nanoparticle (HANP) morphology on embryological development in a zebrafish exposure model. Four distinct HANP morphologies (dots, long rods, sheets, and fibers) were fabricated and characterized. Zebrafish embryos were exposed to HANPs (0–100 mg/L), and viability and developmental deformities were evaluated for up to 5 days post-fertilization (dpf). Malformations such as pericardial edema and axial curvature were apparent in embryos as early as 1 dpf, following exposure to the dot and fiber particles, and developed in embryos by 3 dpf in the sheet and long rod particle groups. Minimal death was observed in response to dot, long rod, and sheet particles (≤25%), while fiber particles induced overwhelming toxicity (≤60%) after 1 dpf, and complete toxicity during all subsequent time points. Collectively, these results suggest that nanoparticle morphology can significantly impact embryological development and should be a required consideration when designing nanomaterials for commercial use.

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“…Such apoptotic effects observedin HepG2 cells were the basis for proposing HA as utilizable in the treatment of hepatoma and other types of cancer [62]. Needle-shaped HA also slowed down the cell metabolism and inhibited hatching in catfish T-cells and zebrafish embryos in a dose-dependent manner [63]. Other studies suggested good biocompatibility and a lack of cytotoxic effects of needle-shaped HA when tested in vitro [64,65], demonstrating a great degree of variability of the biological response to such morphologies.…”

Section: Resultsmentioning

confidence: 99%

Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies

Stojanović

Ignjatović

et al. 2016

Materials Science and Engineering: C

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Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.

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Evaluating the Toxicity of Hydroxyapatite Nanoparticles in Catfish Cells and Zebrafish Embryos

Cited by 48 publications

References 52 publications

Influence of copper oxide nanoparticle form and shape on toxicity and bioaccumulation in the deposit feeder, Capitella teleta

Influence of copper oxide nanoparticle form and shape on toxicity and bioaccumulation in the deposit feeder, Capitella teleta

The Influence of Hydroxyapatite Nanoparticle Morphology on Embryonic Development in a Zebrafish Exposure Model

Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies

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