Evaluating the therapeutic effect of Diallyl disulfide compared to that of Alderonate on Glucocorticoids induced osteoporosis in rats: Biochemical and histomorphometric analysis

Abstract

Introduction: The prevalence of Glucocorticoids (GC) use made Glucocorticoid induced osteoporosis (GIO) an important form of secondary osteoporosis. Bisphosphonates (Alderonate) are considered as pharmacological agents in prevention and treatment of GIO. Garlic containing Diallyl disulfide (DAS) has received special attention for its beneficial effects as an antioxidant. The present study attempted to evaluate the Alendronate, DAS, and the combination of Alderonate and DAS effect on bone gene expression of RANKL and OPG, serum biochemical parameters [Ca, P, alkaline phosphatase (ALP)] and histological assessment of tibia in animal model of GIO. Material and Method: Fifty pathogen albino male rats were allocated in five groups:Control group(C), Methyl prednisolone group (M), methyl prednisolone Aldenronate group (A) Methyl prednisolone Diallyl disulfide (D), Diallyl disulfide Alendronate Methyl prednisolone (AD) group.Gene expression studies, biochemical parameters, histological and morphometric studies were assessed for all animals. Results: Among the study groups, Methyl prednisolone exposure provoked decrease in OPG (0.26±0.16) increase in RANKL (3.57±.39) gene expression, decrease in serum ALP(85.38±6.3),Ca(6.41±0.89), P(1.9 ±0.35) levels and decrease in trabecular thickness (57.01±23.22).Alderonate and Dially disulfide concomitant administration was shown to increase OPG(2.84 ±0.53)and decrease in RANKL (0.63 ± 0.27) gene expression, increase serum ALP(187.75±24.93),Ca(10.330 ±.69)and P(3.16 ±0.43) levels,increase trabecular thickness(154.7±31.7)(p<0.001). Conclusion: Diallyl disulfide can add advantage to Alderonate in treatment of glucocorticoid induced osteoporosis.

Comparative histological study of the potential protective effect of Acarbose, Linagliptin and Quercetin on the aorta of type 2 diabetic rat

“…Images were taken at magnification (400X) to measure the thickness of tunica media (TM) in H&E stained sections, the area percentage of elastic fibers in TM in the VVG stained aortic sections and the area percentage of smooth muscle fibers in TM of Trichrome stained sections. Measurements were expressed using NIH Image J (v1.49) software [5,30] . Additionally, aortic specimens were taken and cut into small pieces (1/2-1 mm3), fixed in 3% glutaraldehyde solution, and processed to get ultrathin sections for transmission electron microscope (TEM) examination.…”

Bisphosphonate’s effect on the tongue in adult male albino rats and the possible protective role of rutin: light and scanning electron microscopic study

“…BPs are divided into two types which differ in their mechanism of action: nitrogen-containing bisphosphonates (N-BPs) and non-nitrogen-containing bisphosphonates (non-N-BPs) [ 4 ]. N-BPs possess the most potent antiresorptive impact as alendronate, zoledronic acid, ibandronate, and risedronate [ 5 ].…”