Evaluating the Suitability of Cryoprotectants and Cryopreservation Solutions for Olive Barb, Puntius Sarana Embryos

Rahman, Syed Mahbubur; Ahsan, Md. Nazmul; Abdullah-Al-Mamun,; Hossain, Quazi Zahangir; Rahman, Md. Mostafizur; Hasan, Md. Mehedi; Chisty, Md. Ayaz Hasan

doi:10.1007/s40011-014-0339-2

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…Short-time storage of fish gametes have been developed for many aquaculture species (Hulata and Rothbard, 1979;Christensen and Tiersch, 1996;Linhart et al, 2001;Gisbert and Williot, 2002;Marques and Godinho, 2004;Vuthiphandchai et al, 2009;Bernáth et al, 2017;Ladoktha et al, 2017;Risopatrón et al, 2018;Santos et al, 2018;Beirão et al, 2019) while dealing with Asian walking catfish, Clarias batrachus is scare. Development of a protocol for short-term storage of gametes of this valuable species could have direct bearings on the hatchery operations in several ways including, for example, addressing asynchrony in maturation, reducing the number of viable male fish sacrificed, transportation of gametes to distant place, reducing inbreeding depression and preserving healthy wild stock (Vuthiphandchai et al, 2009;Rahman et al, 2015;Farago et al, 2017). Therefore, this study was conducted to investigate the viability of Asian walking catfish gametes (eggs and sperm) under different storage conditions.…”

Section: Introductionmentioning

confidence: 99%

Short Term Storage of Asian Walking Catfish (Clarias batrachus Linnaeus, 1758) Gametes

Rahman¹,

Alsaqufi²,

Alkhamis³

et al. 2020

AAVS

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The present study was carried out to investigate the viability of gametes from Asian walking catfish (Clarias batrachus) following short-time storage under hatchery condition at room temperature (26-28°C) and at chilled temperature (2-4ºC) using a household refrigerator. Eggs stored at refrigeration for 5 min resulted in a drastic reduction of fertilization success (36%) in comparison with those stored at room temperature (86%). Eggs without water have had significantly higher successes of fertilization than those stored with water in both storage conditions. On the other hand, prolonged potency was observed for sperm stored in refrigerator than those kept outside at room temperature. Sperm kept in refrigerator with both water and dextrose solutions were consistently viable for at least 48 hours. At room temperature, however, sperm diluted with dextrose showed comparatively higher fertilization (up to 50 min, 27-98%) than sperm diluted with water (up to 10 min, 25-87%). Similar results were obtained when the whole testis was stored. Sperm-egg contact time experiment showed a quick fusion and it required only a minute to achieve 100% fertilization. The results of this study provide evidence on the short-time storage of catfish sperm that might be of relevance to hatchery operators including, for example, production of quality seeds by sacrificing less numbers of male individuals.

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