Evaluating the Sapindusrarak DC Chemical compounds for their ability to inhibit the growth of Fusobacterium nucleatum In vitro

Abstract

The development of natural resources in the root canal irrigant in endodontic treatment has increased to answer biocompatibility issues. The Lerak (Sapindusrarak DC) fruit, known in Indonesia as a traditional soap seed, can be used as an alternative irrigant for root canal irrigation. It almost qualifies as an irritant. This study aimed to determine the antibacterial compound of Sapindusrarak DC (S. rarak DC). That Predicts as the alternative to root canal irrigation. Gas Chromatography-Mass Spectrometry (CG - MS) was used to identify the compounds of Sapindusrarak DC. The results of GC/MS analysis show that the Sapindusrarak DC has bioactive components such as benzyl chloride, 1-Dodecanamine, N, N-Dimethyl-, 1-Tetradecanamine,N,N- Dimethyl, 4- (3-Dimethylaminopropoxy) benzaldehyde, 1- (Dimethylamino) –2- Butanol, Acetamide, 2-(Diethylamino)-N- (2,6-Dimethylphenyl, 9-Octadecenoic Acid, Hexadecanoic Acid, Cis- 13-Octadecenoic acid, methyl ester, Tetrahydroquinoline-4,4,-, 6-Octadecenoic acid, 2- (BenzylmethylamiNomethyl) -2-Norbornone, Cis-13-Eicosenoic acid. At the 24-hour incubation period in a 12.5 percent Sapindusrarak DC, only 0.4% of F. nucleatum survived ina 100 percent salivary growth response. While a saliva concentration of 25% resulted in a growth response of 82 percent, F. nucleatum growth was only 18%. The concentration of 6.25% was then added, followed by CHX. At 48 hours, the S.rarak DC of 12.5% and 6.25% had a better growth response to F. nucleatum than other concentrations. However, CHX was still higher, giving a growth response to F. nucleatum (98.42%). This finding indicates that Sapindusrarak DC has the compound antibacterial to prevent F. nucleatumgrowth, of could be a recommendation as an alternative to natural root canal irrigation in the field of endodontic treatment.