Evaluating the role of nondermatophyte fungi as a causative agent of tinea pedis and its relation to diabetes

Hamdino, Mervat; El-Sayed, Sawsan; Taha, Mohammed; Elkady, Eman

doi:10.4103/sjamf.sjamf_30_20

Cited by 2 publications

(2 citation statements)

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“…Yeasts may colonize the skin, hair, and nails but may become pathogenic in patients with poor immunity consequent to diabetes mellitus, vascular diseases, and anemia. 17,31 Accordingly, 25%, 20% and 46% of our patients complained of diabetes mellitus, vascular diseases, and anemia, respectively.…”

Section: Discussionmentioning

confidence: 73%

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Comparison of Fungal Fluorescent Staining and ITS rDNA PCR-based Sequencing with Conventional Methods for Diagnosis of Onychomycosis

Makled¹,

Ghonaim²,

Ali³

et al. 2022

J. Pure Appl. Microbiol.

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Accurate identification of fungal causes for onychomycosis is essential for proper treatment. Presently available laboratory methods show unreliable sensitivity; so there is a requirement for innovative detection techniques. The aim for this work was to assess the efficiencies of fluorescent staining and internal transcribed spacer (ITS) ribosomal DNA (rDNA) polymerase chain reaction (PCR)-based sequencing in comparison to conventional techniques for diagnosis of onychomycosis. Nail specimens obtained from 100 patients with clinically- diagnosed onychomycosis were analyzed. Nail scrapings or clippings were subjected to direct microscopic examination by KOH mount, culture by using Sabouraud’s dextrose agar and histopathological examination with periodic-acid Schiff (PAS). Collected specimens were subsequently examined by fluorescent staining and PCR-based sequencing (30 specimens only) to compare the feasibility, sensitivity and diagnostic accuracy for these two methods. The most frequently isolated fungi were yeasts (39/76: 51.3%), dermatophytes (24/76; 31.6%) and non-dermatophyte molds (NDMs) (13/76; 17.1%). Mixed mycotic infections were recovered from 6% of the collected nail specimens. The positive detection rates were significantly different between KOH examinations (52%), nail plate histology (55%), fungal culture (70%) and fluorescent staining (80%). Considering fungal culture as the gold standard, the most sensitive technique was PCR (100%) followed by fluorescent staining (89%), PAS staining (69%) while the least sensitive technique was KOH mount (53%). Fluorescence staining can be used as a rapid and high-yield technique for identification of fungi in the specimens. PCR-based sequencing was highly sensitive and faster compared to culture. Whenever possible, it enables species identification with higher adequacy.

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Section: Discussionmentioning

confidence: 73%

“…Some previous studies revealed the same trends, where yeasts accounted for 56%, 65%, 43.3%, 66% and 75.9%, respectively. 1,3,10,17,31 On the other hand, Gautam et al 14 reported that dermatophytes were the mostly isolated fungi (60%) followed by NDMs (30%) and finally Candida spp. (10%) in India.…”

Section: Discussionmentioning

confidence: 99%