Background: Non-point source (NPS) pollution, has been dominant in many urban areas, causing nutrient loss, water body pollution and aqueous systems damaging. Among the pollutants, nitrogen is a key nutrient which can cause eutrophication of rivers, lakes and reservoirs. Beijing, the capital of China, has a booming economy and a huge population. Various human activities have affected nitrogen accumulation deeply and caused considerable NPS pollution. This research calculated the annual load of NPS nitrogen in urban area of Beijing City (UABC) in 2005 and simulated its spatial distribution, which may be useful for environmental planning and pollution control of the UABC and cities with intensive human activities. Results: The total NPS nitrogen load of the UABC was is 1083.09 t in 2005. The load of agricultural land, construction land and unused land were 92.04 t, 969.29 t and 21.77 t. As far as spatial distribution is concerned, construction land was with heavy pollution. Chaoyang district, Haidian district and Fengtai district were the most major export regions of NPS nitrogen loads. The loads of them were 376.88 t, 286.87 t and 249.92 t. As for load intensity of NPS nitrogen, the high-load sources were distributed in the Xicheng district and Dongcheng district, of which the load intensities were 1.10 km 2 /t and 1.11 km