Evaluating the Rat Inner Ear: A Technique Using Scanning Electron Microscopy

Voelker, F. A.; Henderson, Celine M.; Macklin, A. W.; Tucker, Walter E.

doi:10.1001/archotol.1980.00790340021005

Cited by 13 publications

(4 citation statements)

References 9 publications

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“…A small hole was drilled into the cochlear wall, centered over the middle of its long axis (cp. Voelker et al, 1980), and part of the perilymph was soaked off using small tissue wicks. FB (n ϭ 4) or DY (n ϭ 2) was tamped into the cochlea and the opening closed with surgical bone wax.…”

Section: Methodsmentioning

confidence: 99%

Olivocochlear neurons sending axon collaterals into the ventral cochlear nucleus of the rat

2000

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Section: Methodsmentioning

confidence: 99%

Olivocochlear neurons sending axon collaterals into the ventral cochlear nucleus of the rat

2000

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“…Cochlear samples were then rinsed with EM Buffer (0.1 M sodium cacodylate) and placed in decalcification buffer (10% EDTA solution containing 1.0% glutaraldehyde in EM buffer) for 3-4 days. The procedures for SEM were similar to the methods reported previously (Mizuta, Hoshino, & Morita, 1990;Voelker, Henderson, Macklin, & Tucker, 1980).…”

Section: Electron Microscopymentioning

confidence: 86%

Targeted deletion of Atoh8 results in severe hearing loss in mice

Tang

Xie

Zhang

et al. 2021

Genesis

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Atoh8, also named Math6, is a bHLH gene reported to have important functions in the developing nervous system, pancreas and kidney. However, the expression pattern and function of Atoh8 in the inner ear are still unclear. To study the function of Atoh8 in the developing mouse inner ear, we performed targeted deletion of Atoh8 by intercrossing Atoh8 lacZ/+ mice. We studied the expression pattern of Atoh8 in the inner ear and found interesting results that Atoh8-null (Atoh8 lacZ/lacZ ) mice were viable but smaller than their littermates and they were severely hearing impaired, which was confirmed by hearing tests (ABR, DPOAE). We collected 129 viable newborns from 18 litters by crossing Atoh8 lacZ/+ mice and found that the distributions of Atoh8 lacZ/+ , Atoh8 lacZ/lacZ and wild type were very close to their expected Mendelian ratio by χ 2 testing. However, no remarkable morphological changes in cochleae in mutant mice were detected under plastic sectioning and electron microscopy. No remarkable differences in the expression of Myosin6, Prestin, TrkC, GAD65, Tuj1, or Calretinin were detected between the mutant mice and the control mice. These findings indicate that Atoh8 plays an important role in the development of normal hearing, while further studies are required to elucidate its exact function in hearing.

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“…Unlike guinea pig, the otic capsule of rat is thick and difficult to remove except by drilling away the bone. 19 The absence of a limiting membrane between the perilymph and the bone of the cochlea has been reported in gerbils and rats. 20,21 These might suggest that the transotic absorption in rat is different from that in guinea pig.…”

Section: Discussionmentioning

confidence: 99%

“…The guinea pig cochlea is embedded in temporal bone and has thin bony walls; these features may influence FITC‐DEX concentration and distribution within the cochlea. Unlike guinea pig, the otic capsule of rat is thick and difficult to remove except by drilling away the bone . The absence of a limiting membrane between the perilymph and the bone of the cochlea has been reported in gerbils and rats .…”

Section: Discussionmentioning

confidence: 99%