The objective of the present study is to understand the effect of chronic low-dose radiation that induces in vivo radio-adaptive response. The animals were exposed chronically to naturally occring radioactive materials (NORM) for one and two months which correspond to 10 &20 mSv which are comparable to the reality of areas with high background natural radiation (HBNR). Radioactivity assessment of samples was performed using a high purity germanium -ray spectrometer. The study to understand the radio-adaptive response was conducted via exposing the experimental animals to a challenge dose of 2 Gy, after continuous exposure to 10 & 20 mSv. Comet assay was performed in addition to some of antioxidant enzymes concentrations (superoxide dismutase (SOD), catalyze enzyme (CAT), reduced and oxidized glutathione (GSH).Chronic exposure to 10 and 20 mSv showed DNA damage as a significant elevation in Comet assay parameters. In addition, the results of the present work showed a low production of antioxidant enzymes CAT, SOD & GSH in both chronic exposures (10 & 20 mSv and challange dose 2 Gy). The exposure to 20 mSv before 2Gy gamma rays resulted in an enhancement of the antioxidant enzymes accompanied with a decrease in free radicals which represent an adaptive response. It can be said that for low radiation doses, which are less than 100 mSv, their effects differ from high doses, so that it can be said that the body has an adaptive response and the application of the linear nonthreshold model must be reviewed for these doses.