Evaluating the probiotic potential and adhesion characteristics of Bacillus spp. isolated from the intestine of Rhynchocypris lagowskii Dybowski

Zhang, Yurou; Wu, Zhenchao; Yu, Mengnan; Zhang, Dongming; Wang, Qiuju; Lin, Lili; Wang, Guiqin; Elsadek, Mahmoud M.; Yao, Qi; Chen, Yuke; Guo, Zhixin

doi:10.1007/s10499-022-00840-8

Cited by 5 publications

(6 citation statements)

References 56 publications

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“…Related results were reported by Aly et al, (2008) who mentioned that B. Subtilis and L. acidophilus inhibited the growth of A. hydrophila in vitro. Bacillus amyloliquefaciens showed higher antimicrobial activity towards all isolated pathogenic bacteria and the highest inhibition zone was recorded in V. anguillarumas 40 mm, these result similar to those obtained by Zhang et al, (2022) and Pereira et al, (2019) who found that Bacillus spp had the ability to produce antimicrobial substances with diverse structural forms. The present investigation indicated that V. paraheamolyticus and V. anguillarum were more sensitive to probiotic bacterial strains while A. veronii and Ps.…”

Section: Antimicrobial Activity Of Probiotic Bacterial Strains Agains...supporting

confidence: 86%

Inhibitory action of probiotic bacteria for some isolated and identified pathogenic bacteria from naturally infected Nile tilapia (Oreochromis niloticusis)

EL-Makhzangy

Ghaly²,

Mahmoud³

et al. 2022

Bulletin of Faculty of Science, Zagazig University

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The high consumption of antibiotic in aquaculture for controlling fish disease lead to antibiotic resistance, so for improving this resistant against infectious disease it could be achieved by using of probiotics as an alternative strategy. Main objective of this study was to revealed the influence of some isolated probiotic bacteria as Bacillus spp and Lactobacillus spp on pathogenic bacteria which isolated from naturally infected Nile tilapia such as Aeromonas spp, Vibrio spp and Pseudomonas spp. Bioactivity of probiotic were measured by the diameter of inhibition zone on disc diffusion method, six probiotic were used for testing their ability to inhibited the growth of pathogenic bacteria, three of them were kindly provided and the other were isolated from pickles, fermentable milk and yogurt, respectively. The result indicates that Vibrio anguillrium recorded high prevalence pathogenic bacteria as 52% of isolates. The highest inhibition zone refers to Bacillus amyloliquefacien SW19 which range from (10-40 mm).

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Section: Antimicrobial Activity Of Probiotic Bacterial Strains Agains...supporting

confidence: 86%

Inhibitory action of probiotic bacteria for some isolated and identified pathogenic bacteria from naturally infected Nile tilapia (Oreochromis niloticusis)

EL-Makhzangy

Ghaly²,

Mahmoud³

et al. 2022

Bulletin of Faculty of Science, Zagazig University

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“…The ability of microorganisms to survive in a simulated gastrointestinal environment is an effective indicator for evaluating probiotics. To navigate the complex digestive tract, probiotics need to possess resilience against the low pH of the gastrointestinal environment, withstand destruction by gastric and intestinal fluids, and adapt to elevated bile concentrations for successful survival and colonization (Zhang et al, 2022). Guo et al (2016) reported that except for B. subtilis, which was isolated from the intestinal tract of grass carp and capable of tolerating strong acidic environments (pH 2.0), the growth of all other Bacillus species was inhibited to varying degrees at the same pH level.…”

Section: Discussionmentioning

confidence: 99%

“…The resilience of strains to simulated gastric and intestinal fluids was evaluated using a modified method (Zhang et al, 2022). To prepare the simulated gastric fluid, 16.4 mL of 10% HCl and 1 mg/ mL pepsin (Solarbio, China) were mixed into LB liquid medium, with the pH adjusted to 3, followed by sterilization through a 0.22 mm membrane filter.…”

Section: Simulated Gastrointestinal Tolerance Assaymentioning

confidence: 99%

Isolation and characterization of probiotic Lysinibacillus species from the gastrointestinal tract of large yellow croaker (Larimichthys crocea)

Yao,

Wang,

Lin

et al. 2024

Front. Mar. Sci.

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The objective of this study was to identify effective strategies for disease prevention and control that circumvent antimicrobial resistance (AMR) issues, thereby facilitating sustainable aquaculture practices. This research focused on isolating probiotic Bacillus strains from the intestinal tract of healthy large yellow croaker (Larimichthys crocea). Among the 11 strains tested, four demonstrated protease and lipase activities. Further testing identified one strain, Lysinibacillus sp. (LYD11), with strong inhibitory activity against Vibrio harveyi and Vibrio alginolyticus, two major pathogens of the large yellow croaker. The screened strains showed more than 45% co-aggregation with five pathogens and were capable of inhibiting pathogen colonization through competition, repulsion, and substitution adhesion of 20.56 to 48.45%, 40.37 to 75.62% and 55.51 to 85.30%, respectively. The hydrophobicity and self-aggregation rates of LYD11 were 82.73% and 49.47%, respectively, indicating a potent ability for adhesion and colonization within the fish intestine. LYD11 demonstrated robust growth under the NaCl 0.5–3.5% and pH range of 6–8 and was unaffected by the gastrointestinal environment. Safety evaluations at high concentrations in grouper, in both injected and fed groups, indicated no adverse effects, as evidenced by the absence of morbidity or mortality. Consequently, Lysinibacillus sp. LYD11 emerges as a potential probiotic candidate for the prevention and control of bacterial diseases in the large yellow croaker.

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“…Notably, the bacterial abundance of Bacillus increased signi cantly after the addition of B. velezensis FLU-1 in the feed. Bacillus is a genus of Gram-positive, rod-shaped bacteria that are commonly found in various environments, including soil, water, and the gastrointestinal tracts of humans and animals [23]. Some strains of Bacillus, such as Bacillus coagulans and Bacillus subtilis, have been studied for their potential health bene ts as probiotics [24].…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of Bacillus velezensis FLU-1 from the intestinal tract of largemouth bass and its use as a feed additive

Yang,

Wang,

Yun

et al. 2024

Preprint

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Probiotics have been widely used in aquaculture and may serve as a potential alternative to antibiotics. Host-derived probiotics are widely used in aquaculture because they are able to adapt to the host intestinal environment more easily than other probiotics. This study was conducted to evaluate the probiotic potential of the bacteria isolated from the gut of largemouth bass. The actions of dietary supplementation with B. velezensis FLU-1 were investigated in the largemouth bass with respect to growth, the morphology of the intestine, digestive and immune functions, and antioxidant capacity, as well as intestinal microbiota. The results revealed that B. velezensis FLU-1 exhibited promising probiotic traits, including extracellular enzyme production, ability to withstand acidic conditions, high bile salt concentration, and elevated temperatures. Furthermore, it demonstrated high hydrophobicity and auto-aggregation cability, alongside being free from antibiotic resistance and displaying a non-hemolytic nature. A diet with host–derived B. velezensis FLU-1 supplementation improved the growth performance of the fish. It also increased the length of the intestinal villi and tight junction gene expression levels, including claudin-2, occludin, and ZO-1. Host–derived B. velezensis FLU-1 supplementation enhanced the activities of protease, α-amylase, lipase, alkaline phosphatase, acid phosphatase, lysozyme, catalase, glutathione peroxidase, decreased the level of MDA, increased the level of the anti-inflammatory cytokine TGF-β, and decreased the level of the pro-inflammatory cytokine TNF-α. Furthermore, B. velezensis FLU-1 increased the levels of several probiotics, including Lactobacillus and Lactococcus, and bacteria that produce short-chain fatty acids, including Faecalibacterium, Bacteroides, and Clostridium. The results in vivo show that adding B. velezensis FLU-1 to the feed could reduce the mortality of largemouth bass after infection with A. hydrophila, as well as reduce the bacterial load in the spleen.The results indicated that further study is warranted concerning the use of B. velezensis FLU-1 combined with sodium gluconate as a diet supplement in other economically viable fish.

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Evaluating the probiotic potential and adhesion characteristics of Bacillus spp. isolated from the intestine of Rhynchocypris lagowskii Dybowski

Cited by 5 publications

References 56 publications

Inhibitory action of probiotic bacteria for some isolated and identified pathogenic bacteria from naturally infected Nile tilapia (Oreochromis niloticusis)

Inhibitory action of probiotic bacteria for some isolated and identified pathogenic bacteria from naturally infected Nile tilapia (Oreochromis niloticusis)

Isolation and characterization of probiotic Lysinibacillus species from the gastrointestinal tract of large yellow croaker (Larimichthys crocea)

Screening and identification of Bacillus velezensis FLU-1 from the intestinal tract of largemouth bass and its use as a feed additive

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