Evaluating the Potential of Culms from Sugarcane and Energy Cane Varieties Grown in Argentina for Second-Generation Ethanol Production

Kane, Aissata Ousmane; Pellergini, Vanessa O. Arnoldi; Santo, Melissa C. Espirito; Ngom, B.D.; García, José María Ros; Acevedo, Alberto; Erazzú, Luis Ernesto; Polikarpov, Igor

doi:10.1007/s12649-021-01528-5

Cited by 14 publications

(8 citation statements)

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“…al. 2021;Kane et al 2021). Interestingly, NA 78-724 and NA 56 − 30, the two Argentinean hybrids genetically developed under the same sugarcane breeding program did not share a same cluster in any of the matrices analysed.…”

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confidence: 90%

ddRADseq-mediated detection of genetic variants in sugarcane

et al. 2022

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Sugarcane (Saccharum sp.), a world-wide known feedstock for producing sugar, bioethanol, and energy, has an extremely complex genome due to its highly polyploid and aneuploid nature. A double digestion restriction site-associated DNA sequencing protocol (ddRADseq) was tested in four commercial sugarcane hybrids and one high-bre biotype for the detection and potential application of single nucleotide polymorphisms (SNPs) in genetic breeding. This genotyping approach compared two Illumina sequencing platforms and different ltering schemes, with and without a reference genome. A greater number of reads were obtained with Illumina platform Novaseq6000 than with Nextseq500, being this consistent with a greater number of SNPs determined with high accuracy. Additionally, more SNPs were found using a denovo pipeline compared to de refmap pipeline of the Stacks software. Longer read size, paired-end reads and 4M reads per individual represent the most e cient combination in recovering SNPs. The optimal combination of lter parameters varied depending on the different matrices created based on the different platforms. In both matrices, the highest number of SNPs localized in the longest chromosome 1, whereas the fewest landed in the shortest chromosomes 5 and 8. Multivariate comparisons of the SNPs matrices showed closer relationships among commercial hybrids than with the high-bre biotype. Functional analysis of the obtained SNPs, performed with the Variant Effect Predictor, demonstrated the appearance of variants throughout the sugarcane genome. The pipeline applied in this study can be exploited to identify useful molecular markers that can be used in sugarcane breeding programs to reduce selection cycles.

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ddRADseq-mediated detection of genetic variants in sugarcane

et al. 2022

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“…During alkaline pretreatment, alkali is expected to cleave hydrolysable linkages in lignin and glycosidic bonds of polysaccharides, causing a degree of structure. In addition, alkaline saponification of acetyl and uronic ester bonds also improves the polysaccharides' enzymatic accessibility polysaccharides, allowing effective delignification, as found in our previous works (99,188). NaOH penetrates the interface between lignin and polysaccharides, cleaves ester bonds between them, and promotes lignin solubilization (189).…”

Section: Declaration Of Competing Interestmentioning

confidence: 72%

“…In the latter case, initial crystallinity of the untreated sample was quite high and practically remained the same after applied pretreatments (Table 3). After the two-step pretreatments CrIs of all the samples reached 52%-54% level, which are characteristic of well-pretreated and hydrolytically accessible lignocellulosic samples (Brar et al., 2020;Kane et al, 2022).…”

Section: 5x-ray Diffraction Analysismentioning

confidence: 94%

“…Besides, maximum lignin and hemicellulose removal can occur by adding individual features of the different pretreatments. Currently, other configurations of combined pretreatments such as acid/alkaline (99,100), ozonolysis / liquid hot water (101), alkaline/enzyme (102), etc., have been implemented, and excellent yields of fermentable sugar were obtained after enzymatic hydrolysis.…”

Section: Combined Pretreatmentmentioning

confidence: 99%

“…3) clearly indicate the compact structure of leaves is lost because of the applied pretreatments and the cellulose bundles separate creating much bigger interstitial water-accessible spaces, which in our T2 analyses manifest themselves as apparent "lumen" volumes. Separation of cellulose bundles and an increase in their accessibility to enzymes typically result in higher enzymatic hydrolysis yields (Kane et al., 2022;Weiss et al, 2017;, which indicates higher potential susceptibility of SCL_EC3116 samples to the enzymatic degradation.…”

Section: Low-field Nuclear Magnetic Resonancementioning

confidence: 99%

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