Evaluating the potential of chitosan/poly(vinyl alcohol) membranes as alternative carrier material for proliferation of Vero cells

Abstract

Chitosan/poly(vinyl alcohol) (CS/PVA) blend membranes were prepared using the casting method and their physiochemical properties were analyzed using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and X-ray diffraction (XRD). FTIR and XRD demonstrated possible hydrogen bonds between CS and PVA. The addition of PVA to CS resulted in surface roughness as analyzed by SEM. The CS/PVA blend membrane exhibited high tensile properties (81.62%) and reduced water-holding capacity (53.8%) compared to a pure CS membrane (control). Cell viability and proliferation were assessed via an MTT assay with Vero cell culture. Associated with improved physicochemical properties, the CS/PVA blend membrane promotes cell proliferation of Vero cells with high specific growth rate (0.582 day -1 ). The results demonstrate that the blending of CS and PVA could significantly alter the surface rugosity, water-holding capacity and improve the mechanical and biological properties of the membrane. Interestingly, this concept can be extended for different anchorage-dependent cell lines, as an alternative carrier material.

Antimicrobial and Wound Healing Properties of FeO Fabricated Chitosan/PVA Nanocomposite Sponge

“…Another polymer, PVA, also exhibited the two major crystalline peaks at 2θ angle 11.6 • and 19.7 • , and the obtained crystal peaks were consistent with earlier reports [48]. The CS blended PVA exhibited the two crystalline peaks at 8.87 • and 19.83 • , this indicates the conformational transition within the two polymers [49]. Moreover, CS/PVA-PD-FeO NPs (0.01%) composites showed all the crystalline peaks and plane of PD-FeO NPs at 30 4B).…”

“…Another polymer, PVA, also exhibited the two major crystalline peaks at 2θ angle 11.6° and 19.7°, and the obtained crystal peaks were consistent with earlier reports [ 48 ]. The CS blended PVA exhibited the two crystalline peaks at 8.87° and 19.83°, this indicates the conformational transition within the two polymers [ 49 ]. Moreover, CS/PVA-PD-FeO NPs (0.01%) composites showed all the crystalline peaks and plane of PD-FeO NPs at 30.09° (220), 35.50° (311), 43.09° (400), 53.55° (422), 57.20° (511), 62.75° (440), and 74.41° (533), while the peak intensity was lowered without any crystalline phase change of PD-FeO NPs ( Figure 4 B).…”

Comparative evaluation of four triterpenoid glycoside saponins of bacoside A in alleviating sub-cellular oxidative stress of N2a neuroblastoma cells

“…Cells were supplemented with 10 μl of different bacoside viz . bacoside A3, bacopaside II, jujubogenin isomer of bacopasaponin C, bacopasaponin C and bacoside A in the concentration range of (0–1.0 mg/ml each, with 0.2 increment); and supplemented in humidified CO 2 incubator at 37°C for 3 h. After the treatment, cell viability was analysed using MTT assay reported previously with modification. Briefly, 10‐μl MTT (5 mg/ml) added in each well with cells and bacoside A, and incubated for 3 h at 37°C in humidified CO 2 incubator.…”

Effect of carbon sources on physicochemical properties of bacterial cellulose produced from Gluconacetobacter xylinus MTCC 7795

“…Results showed variations in specific growth rate and doubling time of G. xylinus MTCC 7795 with different carbon sources (Table 1). Specific growth rate of bacteria was calculated as reported previously (27,28) using Eq. 1.…”

“…It is also related to doubling time (t d ) of an organism by Eq. (2) (27,28). Doubling time of bacteria was calculated by Eq.…”