Evaluating the performance of gene-based tests of genetic association when testing for association between methylation and change in triglyceride levels at GAW20

Woude, Jason Vander; Huisman, Jordan; Berg, Lucas Vander; Veenstra, Jenna; Bos, Abbey; Kalsbeek, Anya; Koster, Karissa; Ryder, Nathan; Tintle, Nathan L.

doi:10.1186/s12919-018-0124-y

Cited by 2 publications

(1 citation statement)

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“…In contrast, candidate gene-based association studies use genetic variation in genes known or suspected to play a functional role in a phenotypic trait of interest [ 15 , 17 , 22 ]. This approach has appealing features, including reducing the substantial multiple testing penalties required for GWAS and directly connecting statistical testing with functional biological units [ 23 , 24 ]. Several studies have successfully identified associations between SNPs in candidate genes and phenotypic traits in plants such as sugarcane [ 25 ], rubber tree [ 26 ], wheat [ 27 ], and ryegrass [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

A Large-Scale Candidate-Gene Association Mapping for Drought Tolerance and Agronomic Traits in Sugarcane

Wirojsirasak

Songsri

Jongrungklang

et al. 2023

IJMS

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Dissection of the genetic loci controlling drought tolerance traits with a complex genetic inheritance is important for drought-tolerant sugarcane improvement. In this study, we conducted a large-scale candidate gene association study of 649 candidate genes in a sugarcane diversity panel to identify genetic variants underlying agronomic traits and drought tolerance indices evaluated in plant cane and ratoon cane under water-stressed (WS) and non-stressed (NS) environments. We identified 197 significant marker-trait associations (MTAs) in 141 candidate genes associated with 18 evaluated traits with the Bonferroni correction threshold (α = 0.05). Out of the total, 95 MTAs in 78 candidate genes and 62 MTAs in 58 candidate genes were detected under NS and WS conditions, respectively. Most MTAs were found only in specific water regimes and crop seasons. These MTAs explained 7.93–30.52% of phenotypic variation. Association mapping results revealed that 34, 59, and 104 MTAs involved physiological and molecular adaptation, phytohormone metabolism, and drought-inducible genes. They identified 19 pleiotropic genes associated with more than one trait and many genes related to drought tolerance indices. The genetic and genomic resources identified in this study will enable the combining of yield-related traits and sugar-related traits with agronomic value to optimize the yield of sugarcane cultivars grown under drought-stressed and non-stressed environments.

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Section: Introductionmentioning

confidence: 99%

A Large-Scale Candidate-Gene Association Mapping for Drought Tolerance and Agronomic Traits in Sugarcane

Wirojsirasak

Songsri

Jongrungklang

et al. 2023

IJMS

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Application of novel and existing methods to identify genes with evidence of epigenetic association: results from GAW20

et al. 2018

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BackgroundThe rise in popularity and accessibility of DNA methylation data to evaluate epigenetic associations with disease has led to numerous methodological questions. As part of GAW20, our working group of 8 research groups focused on gene searching methods.ResultsAlthough the methods were varied, we identified 3 main themes within our group. First, many groups tackled the question of how best to use pedigree information in downstream analyses, finding that (a) the use of kinship matrices is common practice, (b) ascertainment corrections may be necessary, and (c) pedigree information may be useful for identifying parent-of-origin effects. Second, many groups also considered multimarker versus single-marker tests. Multimarker tests had modestly improved power versus single-marker methods on simulated data, and on real data identified additional associations that were not identified with single-marker methods, including identification of a gene with a strong biological interpretation. Finally, some of the groups explored methods to combine single-nucleotide polymorphism (SNP) and DNA methylation into a single association analysis.ConclusionsA causal inference method showed promise at discovering new mechanisms of SNP activity; gene-based methods of summarizing SNP and DNA methylation data also showed promise. Even though numerous questions still remain in the analysis of DNA methylation data, our discussions at GAW20 suggest some emerging best practices.

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Evaluating the performance of gene-based tests of genetic association when testing for association between methylation and change in triglyceride levels at GAW20

Cited by 2 publications

References 4 publications

A Large-Scale Candidate-Gene Association Mapping for Drought Tolerance and Agronomic Traits in Sugarcane

A Large-Scale Candidate-Gene Association Mapping for Drought Tolerance and Agronomic Traits in Sugarcane

Application of novel and existing methods to identify genes with evidence of epigenetic association: results from GAW20

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