Evaluating the performance of fibronectin 1 (FN1), integrin α4β1 (ITGA4), syndecan-2 (SDC2), and glycoprotein CD44 as the potential biomarkers of oral squamous cell carcinoma (OSCC)

Yen, Ching Yu; Huang, Chun-Ming; Hou, Ming‐Feng; Yang, Yi; Chang, Chao Hsiang; Huang, Hurng Wern; Chen, Chung Ho; Chang, Hsueh-Wei

doi:10.3109/1354750x.2012.737025

Cited by 59 publications

(59 citation statements)

References 67 publications

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“…It has been demonstrated that the expression of FN1 proteins was closely related with the development of various malignant tumors of oral squamous cell carcinoma, nasopharyngeal carcinoma, ovarian cancer, renal cancer, and thyroid cancer. [15][16][17][18][19] However, the previous studies did not clarify the roles of FN1 in CRC behaviors. In this study, we identified FN1 as a potential oncogene in CRC based on analysis of public datasets and clinical subjects and investigated the effects of FN1 on the biological behavior of CRC cells in vitro.…”

Section: Discussionmentioning

confidence: 96%

Down‐regulation of FN1 inhibits colorectal carcinogenesis by suppressing proliferation, migration, and invasion

Cai

Liu

Zhang

et al. 2018

J of Cellular Biochemistry

157

122

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Fibronectin 1 (FN1) is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, metastasis, and implicated in various biochemical processes. However, its effects on the development and progression of human cancer, especially colorectal cancer (CRC), are unclear. To evaluate the relationship between the expression of FN1 and the histopathologic parameters of patients with CRC or the proliferation, migration, and invasion of colorectal cancer cell lines, we screened FN1 as a new candidate gene which promotes development of CRC, in an independent dataset (The Human Protein Atlas website). Here, we reported that FN1 was elevated in CRC tissues compared with normal colon tissues. Further, FN1 expression level was correlated with age, lymph vascular invasion, and survival rate. Knockdown of FN1 in two CRC cell lines, LOVO, and SW1116, significantly inhibited cell proliferation, migration and invasion, and induced cell apoptosis. Western blot analysis showed that down-regulation of FN1 significantly decreased the expression of Bcl-2, MMP-9, Twist, and increased the expression of Bax, Caspase-3, and E-cadherin in LOVO and SW1116 cells. Then, we found that the protein ITGA5 was identified as a binding partner of FN1 and ITGA5 overexpression reversed FN1-induced tumorigenesis of CRC in vitro. Taken together, FN1 suppressed apoptosis and promoted viability, invasion, and migration in CRC through interacting with ITGA5. FN1 may be a prognostic factor and potential target for CRC treatment.

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Section: Discussionmentioning

confidence: 96%

Down‐regulation of FN1 inhibits colorectal carcinogenesis by suppressing proliferation, migration, and invasion

Cai

Liu

Zhang

et al. 2018

J of Cellular Biochemistry

157

122

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“…cDNA was reacted with iQ SYBR Green Supermix (Bio‐Rad Laboratories, Hercules, California) for preforming Real‐Time RT‐PCR using a MyiQ real‐time machine (Bio‐Rad). Epithelial‐mesenchymal transition (EMT) signaling such as focal adhesion kinase ( FAK ), E‐cadherin ( E‐cad ), Slug , twist‐related protein 1 ( Twist ), vimentin ( Vim ), N‐cadherin ( N‐cad ), and GAPDH genes were studied by a touch‐down PCR program (50 cycles) 28 . The primer and PCR amplicon information 29–32 is provided in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Peng

Hsiao

Lan

et al. 2020

Environmental Toxicology

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Discovering drug candidates for the modulation of metastasis is of great importance in inhibiting oral cancer malignancy. Although most pomegranate extract applications aim at the antiproliferation of cancer cells, its antimetastatic effects remain unclear, especially for oral cancer cells. The aim of this study is to evaluate the change of two main metastasis characters, migration and invasion of oral cancer cells. Further, we want to explore the molecular mechanisms of action of pomegranate extract (POMx) at low cytotoxic concentration. We found that POMx ranged from 0 to 50 μg/mL showing low cytotoxicity to oral cancer cells. In the case of oral cancer HSC-3 and Ca9-22 cells, POMx inhibits wound healing migration, transwell migration, and matrix gel invasion.Mechanistically, POMx downregulates matrix metalloproteinase (MMP)-2 and MMP-9 Sheng-Yao Peng and Chien-Chou Hsiao contributed equally to this work.

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“…Thus, identifying reliable biomarkers to predict disease-specific survival (DSS) and the recurrence of OSCC is an emerging issue. To date, many studies have attempted to identify novel biomarkers for OSCC, such as fibronectin 1 (FN1) [22], integrin alpha4beta1 (ITGA4) [22], syndecan-2 (SDC2) [22], glycoprotein CD44 [22], AXL [23], matrix metalloproteinases (MMP) 1 and MMP10 [24]. However, many of these studies are limited to the gene expression or protein levels due to a small numbers of patients.…”

Section: Introductionmentioning

confidence: 99%

Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma

Sie

et al. 2016

Oncotarget

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The clinical significance and biological function of DEXD/H box helicase 60 (DDX60) in oral cancer remains unknown. Herein, we evaluated the association of DDX60 expression with tumorigenesis and the prognosis of oral squamous cell carcinoma (OSCC). DDX60 expression was examined by immunohistochemistry on tissue microarray slides of 494 OSCC patients, including 180 buccal mucosal SCC (BMSCC), 241 tongue SCC (TSCC), and 73 lip SCC (LSCC) patients. DDX60 expression was significantly increased in all three subsites of OSCC compared to its expression in tumor adjacent normal tissues. However, its association with tumorigenesis was specific to the oral cavity subsite after the stratification of betel quid chewing, smoking, and drinking. Among OSCC patients, higher levels of DDX60 expression were associated with the male gender, a well-differentiated tumor, advanced stage of disease, and a large tumor size with subsite specific features. LSCC patients with high DDX60 expression levels showed shorter disease-specific survival, particularly those with moderately or poorly differentiated tumors. Additionally, TSCC or OSCC patients with high DDX60 expression showed a poor disease-free survival (DFS), particularly those with moderately or poorly differentiated tumors. Therefore, DDX60 is a novel and unfavorable biomarker for tumorigenesis and prognosis of OSCC in a subsite-specific manner.

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Evaluating the performance of fibronectin 1 (FN1), integrin α4β1 (ITGA4), syndecan-2 (SDC2), and glycoprotein CD44 as the potential biomarkers of oral squamous cell carcinoma (OSCC)

Abstract: FN1 and ITGA4 are potential OSCC biomarkers for tongue/mouth floor and edentulous ridge.

Cited by 59 publications

References 67 publications

Down‐regulation of FN1 inhibits colorectal carcinogenesis by suppressing proliferation, migration, and invasion

Down‐regulation of FN1 inhibits colorectal carcinogenesis by suppressing proliferation, migration, and invasion

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma

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