Evaluating the performance of different multicolumn setups for chromatographic separation of proteins on hydrophobic interaction chromatography media by a numerical study

Unified design of chromatographic separation processes

“…Principles of the process options are described extensively in the literature. The interested reader is referred to (Guiochon et al, 2006) for details of batch chromatography, to (Sainio and Kaspereit, 2009;Kaspereit and Sainio, 2011) for details of steady state recycling chromatography, to (Rajendran et al, 2009) for details of counter-current chromatography, and to (Bochenek et al, 2013;Thiele et al, 2001) for details of crosscurrent schemes.…”

“…It is applied widely for the separation of petrochemicals (Ruthven and Ching, 1989), sugars (Bubnik et al, 2004), and fine chemicals, particularly enantiomers of chiral compounds (Rajendran et al, 2009). As to the cross-current concepts, the annular chromatography is not gained an industrial breakthrough due to rather complex implementation and/or negligible performance benefit compared to the batch process (Guiochon et al, 2006), whereas the CMC concept is applied, for example, in ion exchange (Huckman et al, 2001;Virolainen et al, 2014) and multistep solvent gradient (Antos 2009;Bochenek et al, 2013) separations.…”

Protein separation in carousel multicolumn setup. Performance analysis and experimental validation

“…In our previous study, we have theoretically analyzed separation of multicomponent protein mixtures in carousel multicolumn setup (CMS) under solvent gradient conditions, and compared its efficiency to other continuous systems such as MCSGP and SMB [21]. In CMS systems, the columns are assembled in parallel, which is aimed to increase throughput of the separation process.…”

“…Nevertheless, it causes undesirable effect of internal solvent mixing [21]. The columns in each zone of such systems are equilibrated with the eluent adequate to the function fulfilled by the zone, i.e., protein binding, separation, resin regeneration, etc.…”

“…After the switch in the position of inlet ports, the columns along with the contained liquid are transported into subsequent zones to realize other stages of the process, which requires eluent exchange. This causes internal distortions of gradient shape, which may be detrimental for the separation efficiency [21].…”