Evaluating the impact of suramin additive on CHO cells producing Fc-fusion protein

Lim, Jin-Hyuk; Cha, Hyun-Myoung; Han, Hyejin; Kim, Dong-Il

doi:10.1007/s10529-019-02728-9

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…These substances are mainly divided into the following two categories: (1) carboxylic acids, of which sodium butyrate (NaB) is the most typical representative. Overall, 5 mmol/L NaB can increase the specific production rate of antibodies in low-yielding cell lines and increase the specific production rate of antibodies in high-yielding cell lines by nearly two-fold (Lim et al, 2019); and…”

Section: Other Ingredientsmentioning

confidence: 94%

“…ROS-mediated caspase activation degrades autophagic machinery and induces apoptosis. Suramin can inhibit cell apoptosis and positively affect the yield and quality of fusion protein and has no effect on the aggregation of fusion protein, and the sialic acid content increases by 1.18 times (Lim et al, 2019). The increase in sialization is not due to the increase in nucleotide sugar content but to the inhibition of sialase activity.…”

Section: Cell Apoptosis and Autophagymentioning

confidence: 96%

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Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

Liu

Fan

Lin

et al. 2021

Front. Bioeng. Biotechnol.

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At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.

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Section: Other Ingredientsmentioning

confidence: 94%

Section: Cell Apoptosis and Autophagymentioning

confidence: 96%