Evaluating the immunogenicity of chemically-synthesised peptides derived from foot-and-mouth disease VP1, VP2 and VP3 proteins as vaccine candidates

Avendaño, Catalina; Celis-Giraldo, Carmen Teresa; Ordóñez, Diego; Díaz-Arévalo, Diana; Rodríguez-Habibe, Ibett; Oviedo, Jairo; Curtidor, Hernando; García-Castiblanco, Sebastián; Martínez-Panqueva, Fredy; Camargo-Castañeda, Andrea M.; Reyes, César; Bohórquez, Michel David; Vanegas, Magnolia; Cantor, Daniela; Patarroyo, Manuel Elkín; Patarroyo, Manuel A.

doi:10.1016/j.vaccine.2020.04.006

Cited by 7 publications

(4 citation statements)

References 49 publications

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“…For insertion, the structural protein VP1 of the FMDV and the outer membrane protein 25 of Brucella were selected. The FMDV structural protein VP1 is an important antigen and is used for the development of recombinant vaccines [ 29 , 30 ]. Brucella OMP25 protein is a protective antigen for brucellosis and is used in combination with other bacterial proteins to develop anti-brucellosis vaccines [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Chervyakova¹,

Tailakova²,

Kozhabergenov³

et al. 2021

Microorganisms

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Capripoxviruses with a host range limited to ruminants have the great potential to be used as vaccine vectors. The aim of this work was to evaluate attenuated sheep pox virus (SPPV) vaccine strain NISKHI as a vector expressing several genes. Open reading frames SPPV020 (ribonucleotide kinase) and SPPV066 (thymidine kinase) were selected as sites for the insertion of foreign genes. Two integration plasmids with expression cassette were designed and constructed. Recombinant SPPVs expressing an enhanced green fluorescent protein (EGFP) (rSPPV(RRΔ)EGFP and rSPPV(TKΔ)EGFP), Foot-and-mouth disease virus capsid protein (VP1), and Brucella spp. outer membrane protein 25 (OMP25) (rSPPV(RRΔ)VP1A-(TKΔ)OMP25) were generated under the transient dominant selection method. The insertion of foreign genes into the SPPV020 and SPPV066 open reading frames did not influence the replication of the recombinant viruses in the cells. Successful foreign gene expression in vitro was assessed by luminescent microscopy (EGFP) and Western blot (VP1 and OMP25). Our results have shown that foreign genes were expressed by rSPPV both in permissive (lamb testicles) and non-permissive (bovine kidney, saiga kidney, porcine kidney) cells. Mice immunized with rSPPV(RRΔ)VP1A-(TKΔ)OMP25 elicited specific antibodies to both SPPV and foreign genes VP1 and OMP25. Thus, SPPV NISKHI may be used as a potential safe immunogenic viral vector for the development of polyvalent vaccines.

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Section: Discussionmentioning

confidence: 99%

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Chervyakova¹,

Tailakova²,

Kozhabergenov³

et al. 2021

Microorganisms

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“…P1 polypeptide expression, however, created a modest quantity of empty virus capsids in vaccines and baculoviruses that require the necessary 3C P1 processing. The expression of virus protein in vector and DNA replication vaccines The development of recombinant replicating Vector Viral Immunogens is an approach for generating the vast range of immune responses that occur during viral infection in an effective way [31].…”

Section: Viral Protein and Peptide Vaccinesmentioning

confidence: 99%

Foot and mouth disease virus: A review

Jumaa¹,

Mohsin²,

Abdulmjeed³

et al. 2021

Magna Sci. Adv. Biol. Pharm.

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As seen by prior tragic outbreaks in many places throughout the world, the foot and mouth disease virus, or "FMDV," is one of the most critical challenges in animal health. In this review, the major features of FMDV, as well as aspects of its interactions with cells and hosts, were discussed. On the other hand, present and upcoming FMD treatment approaches. The first vertebrate virus found was the foot-and-mouth disease virus (FMDV). A capsid protein and the viral genome (+ve sense single strand RNA) make up FMDV. The icosahedral symmetry of the viral structure is made up of structural proteins (VP1, VP2, VP3, and VP4) as well as non-structural proteins (L, 1A, 1B, 1C, 1D, 2A, 2B, 2C, 3A, 3B, 3C, and 3D). The viral replication takes place in the cytoplasm of the cell. Because FMDV has a short incubation period, it spreads quickly. Direct contact is the most often used method of FMDV transmission. The occurrence of direct contact via aerosol and mechanical transmission (fomites, feed, and water). The immunological response is stimulated by the infection with FMD. However, due to virus antigenic diversity, the immune response does not always protect against FMD (antigenic shift). FMDV is divided into seven serotypes based on antigenic variation. O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1 are the serotypes in question. O is the most frequent serotype.

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“…3 Additional variations, called topotypes, also emerge due to the rapid mutation of their short single-stranded RNA genomes. 4 The wide antigenic diversity among these multiple serotypes and their variants makes disease control extremely challenging. Therefore, a rapid on-site diagnosis capable of real-time tracking for the detection of FMDV is needed to prevent the further spread of the disease.…”

mentioning

confidence: 99%

“…FMD virus (FMDV) exists in 7 serotypes: O, A, Asia 1, C, South African territories 1 (SAT1), SAT2, and SAT3 . Additional variations, called topotypes, also emerge due to the rapid mutation of their short single-stranded RNA genomes . The wide antigenic diversity among these multiple serotypes and their variants makes disease control extremely challenging.…”

mentioning

confidence: 99%

On-Site Remote Monitoring System with NIR Signal-Based Detection of Infectious Disease Virus in Opaque Salivary Samples

Kim

Ryoo

Park³

et al. 2023

ACS Sens.

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Infectious disease viruses, such as foot-and-mouth disease virus (FMDV), are highly contagious viruses that cause significant socioeconomic damage upon spreading. Developing an on-site diagnostic tool for early clinical detection and real-time surveillance of FMDV outbreaks is essential to prevent the further spread of the disease. However, early diagnosis of FMDV is still challenging due to the limited sensitivity and time-consuming manual result entry of commercial on-site tests for salivary samples. Here, we report a near-infrared (NIR) signal nanoprobe-based highly accurate detection and remote monitoring system toward FMDVs, which automates the analysis and reporting of diagnosis data. The NIR signal lateral flow immunoassay (LFA) was assembled with a nanoprobe with a stable emission intensity at 800 nm, minimizing the interference signal of opaque salivary samples. We investigated the clinical applicability of the NIR signal LFA at biosafety level 3 (BSL-3) laboratories using 147 opaque salivary samples. The NIR signal LFA achieved a 32-fold lower limit of detection (LOD) than a commercial LFA in detecting live FMDVs, including all isolates occurring in the Republic of Korea during 2010–2017. Our results showed that the NIR signal LFA successfully discriminated the FMDV-positive clinical salivary samples from healthy controls with a sensitivity of 96.9%, specificity of 100.0%, and AUC (area under the receiver operator characteristic curve) value of 0.999. Finally, we substantiated the real-time collection of diagnostic results using a customized portable NIR reader at nine different laboratories of government-certified quarantine institutions for foot-and-mouth disease (FMD).

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Evaluating the immunogenicity of chemically-synthesised peptides derived from foot-and-mouth disease VP1, VP2 and VP3 proteins as vaccine candidates

Cited by 7 publications

References 49 publications

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Foot and mouth disease virus: A review

On-Site Remote Monitoring System with NIR Signal-Based Detection of Infectious Disease Virus in Opaque Salivary Samples

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