Evaluating the Genetic Role of Circadian Clock Genes in Parkinson’s Disease

Xiang, Yaqin; Huang, Juanjuan; Wang, Yige; Huang, XiuRong; Zeng, Qian; Liu, Lizhi; Zhao, Yuwen; Pan, Hongxu; Xu, Qian; Liu, Zhenhua; Sun, Qiying; Wang, Junling; Tan, Jieqiong; Shen, Lu; Jiang, Hong; Yan, Xinxiang; Li, Jinchen; Tang, Beisha; Guo, Jifeng

doi:10.1007/s12035-023-03243-9

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“…11 Recent studies have demonstrated that PER2 plays a pivotal role in several contexts and paradigms, such as inflammatory conditions in the intestine and eyes, cholesterol metabolism, Parkinson's disease, infertility, and early senescence. [12][13][14][15][16][17] However, the specific molecular mechanisms by which anomalous expression of PER2 interferes with tissue repair have remained unclear. In this study, we investigated the role of PER2 in tissue repair in vivo by depleting PER2 and analyzing the skin's response to injury using a well-established skin injury model.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have demonstrated that PER2 plays a pivotal role in several contexts and paradigms, such as inflammatory conditions in the intestine and eyes, cholesterol metabolism, Parkinson's disease, infertility, and early senescence 12–17 . However, the specific molecular mechanisms by which anomalous expression of PER2 interferes with tissue repair have remained unclear.…”

Section: Introductionmentioning

confidence: 99%

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Clock gene Per2 modulates epidermal tissue repair in vivo

Yujra,

Silveira,

Ribeiro

et al. 2024

J of Cellular Biochemistry

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Wound healing can be influenced by genes that control the circadian cycle, including Per2 and BMAL1, which coordinate the functions of several organs, including the skin. The aim of the study was to evaluate the role of PER2 during experimental skin wound healing. Two groups (control and Per2‐KO), consisting of 14 male mice each, were anesthetized by inhalation, and two 6 mm wounds were created on their dorsal skin using a punch biopsy. A silicone ring was sutured around the wound perimeter to restrict contraction. The wound healing process was clinically measured daily (closure index) until complete wound repair. On Day 6, histomorphometric analysis was performed using the length and thickness of the epithelial migration tongue, in addition to counting vessels underlying the lesion by immunofluorescence assay and maturation of collagen fibers through picrosirius staining. Bromodeoxyuridine (BrdU) incorporation and quantification were performed using the subcutaneous injection technique 2 h before euthanasia and through immunohistochemical analysis of the proliferative index. In addition, the qualitative analysis of myofibroblasts and periostin distribution in connective tissue was performed by immunofluorescence. Statistically significant differences were observed in the healing time between the experimental groups (means: 15.5 days for control mice and 13.5 days for Per2‐KO; p = 0.001). The accelerated healing observed in the Per2‐KO group (p < 0.05) was accompanied by statistical differences in wound diameter and length of the migrating epithelial tongue (p = 0.01) compared to the control group. Regarding BrdU immunoreactivity, higher expression was observed in the intact epithelium of Per2‐KO animals (p = 0.01), and this difference compared to control was also present, to a lesser extent, at the wound site (p = 0.03). Immunofluorescence in the connective tissue underlying the wound showed a higher angiogenic potential in the Per2‐KO group in the intact tissue area and the wound region (p < 0.01), where increased expression of myofibroblasts was also observed. Qualitative analysis revealed the distribution of periostin protein and collagen fibers in the connective tissue underlying the wound, with greater organization and maturation during the analyzed period. Our research showed that the absence of the Per2 gene positively impacts the healing time of the skin in vivo. This acceleration depends on the increase of epithelial proliferative and angiogenic capacity of cells carrying the Per2 deletion.

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