Evaluating the feasibility of five candidate DNA Barcoding Loci for Philippine Lasianthus Jack (Lasiantheae: Rubiaceae)

Abstract

Introduction:The pantropical genus Lasianthus Jack is identified for high phenotypic plasticity making traditional taxonomic identification difficult. Having some members with important medicinal properties, a precise complimentary identification through DNA barcoding is needed for species delineation.Materials and Methods:In this study, 12 samples representing six Philippine Lasianthus species were used to determine the most efficient barcoding loci among the cpDNA markers (matK, rbcL, rps16, and trnT-F) and nrDNA (ITS) based on the criteria of universality, discriminatory power, and resolution of species.Results:The results revealed that ITS has the recommended primer universality, greatest interspecific divergences, and average resolution of species. Among the cpDNA markers, matK and rbcL are recommended but with minimal resolution of species. While trnT-F showed moderate interspecific variations and resolution of Lasianthus species, rps16 has the lowest interspecific divergence and resolution of species.Conclusion:Consequently, ITS is the potential ideal DNA barcode for Lasianthus species.SUMMARY ITS, matK, and rps16 markers have the excellent amplification and sequence qualityITS marker has the highest interspecific divergence with the maximum values, followed by matK, rbcL, trnT-F, and rps16, respectivelyAll markers except rps16 yielded average resolution to Lasianthus speciesITS marker is the most ideal locus in terms of excellent universality, high interspecific discriminatory ability, and average species resolution. Abbreviations used: ITS: Internal Transcribe Spacer, matK: maturase K, rbcL: ribulose-1,5-biphospahte-carboxylase, rps16: ribosomal protein 16 small subunit gene.

Decoding Evolution of Rubioideae: Plastomes Reveal Sweet Secrets of Codon Usage, Diagnostides, and Superbarcoding

“…The organellar genomes of Rubiaceae species have never been used as superbarcodes, and scientists have mainly worked with nuclear DNA fragments such as internal transcribed spacers (ITS) and single genes or regions of plastome: matK, trnH-psbA, rbcL, rps16, ndhF, petD, and trnT-trnF [60][61][62][63]. Normally, superbarcodes should be diverse enough to distinguish interspecies differences, so the mitochondrial genome is very rarely used in plant species delimitation.…”

Five triterpenoids from the stems of Lasianthus bidoupensis V.S. Dang & Naiki (Rubiaceae)

“…1 Các cây cùng chi Lasianthus đã được sử dụng trong Y học cổ truyền điều trị bệnh ù tai, viêm khớp, dùng để hạ sốt, an thần, cầm máu, dùng làm thuốc giải độc gan, lợi tiểu. 2,3 Các nghiên cứu cũng cho thấy chi Lasianthus có hoạt tính chống oxi hóa, chống khối u, chống viêm, chống ung thư buồng trứng. [4][5][6] Các hợp chất thuộc khung anthraquinone, iridoid, terpenoid, steroid, phenolic cũng đã được tìm thấy có trong chi Lasianthus.…”

“…epi-betulinic acid (2) và 3-epi-3-O-acetyl betulinic acid(3)] và hai triterpen khung oleanane [3epi-oleanolic acid (4) và vergatic acid(5)]. Ba hợp chất 1, 2 và 3 được thử nghiệm hoạt tính gây độc tế bào MCF-7 trong đó hợp chất 3 cho hoạt tính mạnh nhất với IC 50 là 13,88±0,11 mg/mL.…”