Evaluating the efficacy of endolysins and membrane permeabilizers against Vibrio parahaemolyticus in marine conditions

Zermeño-Cervantes, Lina Angélica; Díaz, Sergio Francisco Martínez; Venancio-Landeros, Alberto Antony; Cardona-Félix, César S.

doi:10.1016/j.resmic.2023.104104

Cited by 5 publications

(3 citation statements)

References 60 publications

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“…Consequently, the differences in activity observed across different strains in our study and previous reports are primarily attributed to variances in susceptibility to permeabilization treatment rather than disparities in their cell wall composition, which remains relatively consistent among Gram-negative bacteria [58][59][60]. In this context, Zermeño-Cervantes [61] evaluated the efficacy of KZ144 and LysPA26 endolysins against V. parahaemolyticus in natural seawater. The results revealed that both endolysins displayed lytic activity in seawater, although KZ144 exhibited a reduction compared to the control condition.…”

Section: Discussionsupporting

confidence: 57%

“…In the case of shrimp larvae, the early application of phage mixture (at 6 h post-infection) was effective to avoid mortality (80% survival) [67]. The use of endolysins for the control of early shrimp disease (AHPND), caused by Vibrio, is still in the evaluation stage of in vitro trials on the different strains reported to be associated with this disease [61].…”

Section: Discussionmentioning

confidence: 99%

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Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Romero,

Blas-Chumacero,

Urzúa

et al. 2024

Microorganisms

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Vibrio species are naturally found in estuarine and marine ecosystems, but are also recognized as significant human enteropathogens, often linked to seafood-related illnesses. In aquaculture settings, Vibrio poses a substantial risk of infectious diseases, resulting in considerable stock losses and prompting the use of antimicrobials. However, this practice contributes to the proliferation of antimicrobial-resistant (AMR) bacteria and resistance genes. Our investigation aimed to explore the potential of biological agents such as bacteriophage CH20 and endolysin LysVPp1 in reducing Vibrio bacterial loads in both rotifer and fish larvae. LysVPp1’s lytic activity was assessed by measuring absorbance reduction against various pathogenic Vibrio strains. Phage CH20 exhibited a limited host range, affecting only Vibrio alginolyticus GV09, a highly pathogenic strain. Both CH20 and LysVPp1 were evaluated for their effectiveness in reducing Vibrio load in rotifers or fish larvae through short-setting bioassays. Our results demonstrated the significant lytic effect of endolysin LysVPp1 on strains of Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio splendidus. Furthermore, we have showcased the feasibility of reducing the load of pathogenic Vibrio in live feed and fish larvae by using a non-antibiotic-based approach, such as lytic phage and endolysin LysVPp1, thus contributing to the progress of a sustainable aquaculture from a One Health perspective.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Romero,

Blas-Chumacero,

Urzúa

et al. 2024

Microorganisms

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“…However, in the case of Gram-negative bacteria and mycobacterial spp., the outer membrane/envelope acts as a barrier for enzymes to access the peptidoglycan [14,15]. Hence, interventions such as outer membrane permeabilizers are required [16,17,18,19].…”

Section: Introductionmentioning

confidence: 99%

A novel LysinB from an F2 sub-cluster mycobacteriophageRitSun

Arora,

Nadar,

Bajpai

2024

Preprint

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With the growing antibiotic resistance in mycobacterial species posing a significant threat globally, there is an urgent need to find alternative solutions. Bacteriophage-derived endolysins aid in releasing phage progeny from the host bacteria by attacking the cell wall at the end of their life cycle. Endolysins are attractive antibacterial candidates due to their rapid lytic action, specificity and low risk of resistance development. In mycobacteria, owing to the complex, hydrophobic cell wall, mycobacteriophages usually synthesize two endolysins: LysinA, which hydrolyzes peptidoglycan; LysinB, which delinks mycolylarabinogalactan from peptidoglycan and releases mycolic acid. In this study, we conducted domain analysis and functional characterization of a recombinant LysinB from RitSun, an F2 sub-cluster mycobacteriophage. Several properties of RitSun LysinB are important as an antimycobacterial agent: its ability to lyse Mycobacterium smegmatis "from without", a specific activity of 1.36 U/mg, higher than the reported ones and its inhibitory effect on biofilm formation. Given the impervious nature of the mycobacterial cell envelope, native endolysins ability to damage cells on exogenous applications warrants further investigation. A molecular dissection of RitSun LysinB to identify its cell wall destabilizing sequence could be utilized to engineer other native lysins as fusion proteins and expand their activity profile.

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