Evaluating the Effects of Tetrachloro-1,4-benzoquinone, an Active Metabolite of Pentachlorophenol, on the Growth of Human Breast Cancer Cells

Ling, Binbing; Gao, Bosong; Yang, Jian

doi:10.1155/2016/8253726

Cited by 11 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Greater activity was observed at longer time (t > 5 h) for the immobilized polymer form as denoted by the arrow in Figure 3a . In Figure 3a , the observed increase in absorbance relates to the formation of various PCP metabolites such as DCHQ and TCBQ, as described elsewhere (Ling et al, 2016 ). The degradation pathway of chlorophenols and other quinones to intermediates/byproducts such as DCHQ and TCBQ with the use of SpC and other microorganisms have been reported (Arora and Bae, 2014 ; Lopez-Echartea et al, 2016 ).…”

Section: Resultssupporting

confidence: 64%

“…As mentioned previously, PCP is genotoxic and a potential carcinogen, where its toxicity is related to the formation of highly reactive metabolites (e.g., TCBQ and DCHQ) (Ling et al, 2016 ). The cytotoxicity assays of hydroquinones are well known in the literature (Li et al, 2015 ).…”

Section: Resultsmentioning

confidence: 96%

“…The cytotoxicity assays of hydroquinones are well known in the literature (Li et al, 2015 ). Herein, the apoptosis (caspase 3/7 level) and cytotoxicity (lactate dehydrogenase level) of DCHQ toward human breast cancer cells were measured with the Caspase-Glo® 3/7 Assay and the CytoTox96® Non-Radioactive Cytotoxicity assay from Promega Corporation (Madison, WI, USA), respectively, using published protocols (Ling et al, 2016 ). DCHQ rapidly forms a stable semi-quinone radical in aqueous solution that induces cell apoptosis and DNA fragmentation in the body.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

2018

Self Cite

View full text Add to dashboard Cite

Dual function polymer materials with immobilized Sphingobium Chorophenolicum (SpC) bacterium cells are reported herein that undergo tandem adsorption and biodegradation of phenolic compounds. The cross-linked polymer materials contain β-cyclodextrin (β-CD) with incremental hexamethylene diisocyanate (HDI) cross-linker at variable mole ratios (X = 1, 3, or 6), denoted as HDI-X systems. The adsorptive uptake properties of the insoluble HDI-X polymers (X = 3 and 6) with various phenolic compounds [pentachlorophenol (PCP), 2,4,6-trichlorophenol (TCP), and 2,4,6-trimethylphenol (TMP)] were studied using batch adsorption isotherms. The molecular selective phenol removal (SR) capacity of the HDI-3 and HDI-6 materials was evaluated by electrospray ionization mass spectrometry (ESI-MS). The results were compared against granular activated carbon (GAC) and native β-CD, where 1D/2D 1H NMR spectral characterization of the complexes formed between phenolic guests and a soluble polymer (HDI-1) in aqueous solution provide insight on the intermolecular interactions and the role of cross-linking effects. Immobilization of SpC onto HDI-3 was shown to form a composite polymer/bacterium material. The composite system displays synergistic removal effects due to tandem PCP adsorption and SpC biodegradation to yield by-products such as 2,6-dichloro-1,4-hydroquinone (DCHQ). Apoptosis and cytotoxicity of DCHQ were evaluated using three breast cancer cell lines.

show abstract

Section: Resultssupporting

confidence: 64%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The intracellular ROS level in human MM A-375 cells was measured using modified published protocol for the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay 35. Briefly, A-375 cells harvested from the T-75 flasks were plated on a black flat-bottom 96-well plate at 10,000 cells per well and incubated at 37°C for 24 hr before being treated with the water extract of S. nigrum and its serial diluted solutions (2-, 4-, 8-, and 16-fold) for another 24 hr.…”

Section: Methodsmentioning

confidence: 99%

Evaluating the cytotoxic effects of the water extracts of four anticancer herbs against human malignant melanoma cells

Ling¹,

Michel²,

Sakharkar³

et al. 2016

DDDT

Self Cite

View full text Add to dashboard Cite

Malignant melanoma (MM) is the most dangerous type of skin cancer, killing more than 1,100 people each year in Canada. Prognosis for late stage and recurrent MM is extremely poor due to insensitivity to chemotherapy drugs, and thus many patients seek complementary and alternative medicines. In this study, we examined four commonly used anticancer herbs in traditional Chinese medicine, Hedyotis diffusa, Scutellaria barbata, Lobelia chinensis, and Solanum nigrum, for their in vitro antitumor effects toward human MM cell line A-375. The crude water extract of S. nigrum (1 g of dry herb in 100 mL water) and its 2-fold dilution caused 52.8%±13.0% and 17.3%±2.7% cytotoxicity in A-375 cells, respectively (P<0.01). The crude water extract of H. diffusa caused 11.1%±12.4% cytotoxicity in A-375 cells with no statistical significance (P>0.05). Higher concentrated formulation might be needed for H. diffusa to exert its cytotoxic effect against A-375 cells. No cytotoxicity was observed in A-375 cells treated with crude water extract of S. barbata and L. chinensis. Further high performance liquid chromatography-tandem mass spectroscopy analysis of the herbal extracts implicated that S. nigrum and H. diffusa might have adopted the same bioactive components for their cytotoxic effects in spite of belonging to two different plant families. We also showed that the crude water extract of S. nigrum reduced intracellular reactive oxygen species generation in A-375 cells, which may lead to a cytostatic effect. Furthermore, synergistic effect was achieved when crude water extract of S. nigrum was coadministered with temozolomide, a chemotherapy drug for skin cancer.

show abstract

“…This type of triterpenoid saponins showed cytotoxicity against a variety of human cancer cell lines through cell cycle arresting, apoptosis inducing and microtubule disassembling, which demonstrate the potential anti-cancer properties of the saponins. [4][5][6][7][8] We have isolated thirteen triterpenoid saponins (1,(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) from the rhizomes of A. gigantifolia STAPF. and obtained four new triterpenoid saponins (2-5) by biotransformation of compound 1.…”

Section: Introductionmentioning

confidence: 99%

Triterpenoid Saponins from <i>Ardisia gigantifolia</i> and Mechanism on Inhibiting Proliferation of MDA-MB-231 Cells

Yan

Wang

et al. 2019

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Seventeen 13,28-epoxy triterpenoid saponins obtained from Ardisia gigantifolia STAPF. were evaluated their anti-proliferative activities on MCF-7 cells. The structure-activity relationship analysis indicated that CH 3 group at C-30, four saccharide units with L-rhamnose at R6 in the sugar units are crucial for the cytotoxic activity on MCF-7. Compounds 1, 2, 6, 7, 12, and 14 were selected to identify the anti-proliferative activity on the other three breast cancer cell lines (T47D, MDA-MB-231 and SK-BR-3). Compounds 2, 6, and 7 with good activity on MCF-7 also showed activity on T47D, MDA-MB-231, and SK-BR-3. Compounds 12 and 14 without cytotoxic activity on MCF-7 almost showed no activities on the other three cell lines. For the triple-negative breast cancer MDA-MB-231, Saponins 7 and 14 showed selective cytotoxic activity, 7 showed much more activity than 14, suggesting the six saccharide units in sugar units and CH 3 on C-30 were the key moieties for the anti-proliferative activities. Further molecular mechanism of saponin 7 was studied on inhibiting cell proliferation of MDA-MB-231 cells. Saponin 7 could enhance apoptosis, arrest cell cycles, decrease mitochondrial membrane potentials (MMPs), and considered the involvement of reactive oxygen species (ROS) may explain this conundrum.

show abstract

Evaluating the Effects of Tetrachloro-1,4-benzoquinone, an Active Metabolite of Pentachlorophenol, on the Growth of Human Breast Cancer Cells

Cited by 11 publications

References 38 publications

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

Evaluating the cytotoxic effects of the water extracts of four anticancer herbs against human malignant melanoma cells

Triterpenoid Saponins from <i>Ardisia gigantifolia</i> and Mechanism on Inhibiting Proliferation of MDA-MB-231 Cells

Contact Info

Product

Resources

About