Evaluating the effect of pupil dilation on spectral-domain optical coherence tomography measurements and their quality score

Tanga, Lucia; Roberti, Gloria; Oddone, Francesco; Quaranta, Luciano; Ferrazza, Manuela; Berardo, Francesca; Manni, Gianluca; Centofanti, Marco

doi:10.1186/s12886-015-0168-y

Cited by 18 publications

(12 citation statements)

References 19 publications

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“…Before imaging, all subjects with ACHM were dilated using either a single drop of cyclomydril (cyclopentolate hydrochloride [0.2%] and phenylephrine hydrochloride [1%]) or a combination of tropicamide (1%) and phenylephrine hydrochloride (2.5%) for cycloplegia and pupillary dilation. Control subjects were imaged without dilation, as a previous study evaluated layer thickness measurements before and after dilation and found no change 30. The Bioptigen spectral domain OCT (Leica Microsystems, Wetzlar, Germany) was used to acquire transfoveal line and/or volume scans in both eyes of each subject.…”

Section: Methodsmentioning

confidence: 99%

Assessing the Interocular Symmetry of Foveal Outer Nuclear Layer Thickness in Achromatopsia

Mastey

Gaffney

Litts

et al. 2019

Trans. Vis. Sci. Tech.

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Purpose: We examine the interocular symmetry of foveal outer nuclear layer (ONL) thickness measurements in subjects with achromatopsia (ACHM). Methods: Images from 76 subjects with CNGA3-or CNGB3-associated ACHM and 42 control subjects were included in the study. Line or volume scans through the fovea of each eye were acquired using optical coherence tomography (OCT). Image quality was assessed for each image included in the analysis using a previously-described maximum tissue contrast index (mTCI) metric. Three foveal ONL thickness measurements were made by a single observer and interocular symmetry was assessed using the average of the three measurements for each eye. Results: Mean (6 standard deviation) foveal ONL thickness for subjects with ACHM was 79.7 6 18.3 lm (right eye) and 79.2 6 18.7 lm (left eye) compared to 112.9 6 15.2 (right eye) and 112.1 6 13.9 lm (left eye) for controls. Foveal ONL thickness did not differ between eyes for ACHM (P ¼ 0.636) or control subjects (P ¼ 0.434). No significant relationship between mTCI and observer repeatability was observed for either control (P ¼ 0.140) or ACHM (P ¼ 0.351) images. Conclusions: While foveal ONL thickness is reduced in ACHM compared to controls, the high interocular symmetry indicates that contralateral ONL measurements could be used as a negative control in early-phase monocular treatment trials. Translational Relevance: Foveal ONL thickness can be measured using OCT images over a wide range of image quality. The interocular symmetry of foveal ONL thickness in ACHM and control populations supports the use of the non-study eye as a control for clinical trial purposes.

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Section: Methodsmentioning

confidence: 99%

Assessing the Interocular Symmetry of Foveal Outer Nuclear Layer Thickness in Achromatopsia

Mastey

Gaffney

Litts

et al. 2019

Trans. Vis. Sci. Tech.

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“…However, dilation is not necessary to get good OMAG signal (unpublished data). Overall, the pupil size does not affect the signal strength of OCT signal 41 , 42 as well as OMAG flow signal (unpublished data).…”

Section: Discussionmentioning

confidence: 87%

Peripapillary Retinal Nerve Fiber Layer Vascular Microcirculation in Glaucoma Using Optical Coherence Tomography–Based Microangiography

Chen

Zhang

Bojikian

et al. 2016

Invest. Ophthalmol. Vis. Sci.

125

113

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PurposeTo investigate the vascular microcirculation changes in the retinal nerve fiber layer (RNFL) in normal, glaucoma suspect, and open-angle glaucoma (OAG) groups using optical coherence tomography–based microangiography (OMAG).MethodsOne eye from each subject was scanned with a Cirrus HD-OCT 5000–based OMAG prototype system montage scanning protocol centered at the optic nerve head (ONH). Blood flow signals were extracted using OMAG algorithm. Retinal nerve fiber layer vascular microcirculation was measured by calculating the blood flux index and vessel area density within a 1.2-mm width annulus centered at the ONH with exclusion of big retinal vessels. One-way ANOVA were performed to analyze the RNFL microcirculation among groups. Linear-regression models were constructed to analyze the correlation between RNFL microcirculation and clinical parameters. Discrimination capabilities of the flow metrics were assessed with the area under the receiver operating characteristic curve (AROC).ResultsTwenty normal, 26 glaucoma suspect, and 42 OAG subjects were enrolled. Eyes from OAG subjects and glaucoma suspects showed significantly lower blood flux index compared with normal eyes (P ≤ 0.0015). Retinal nerve fiber layer blood flow metrics showed significant correlations with visual field indices and structural changes in glaucomatous eyes (P ≤ 0.0123). Similar discrimination capability of blood flux index compared with RNFL thickness was found in both disease groups.ConclusionsPeripapillary RNFL vascular microcirculation measured as blood flux index by OMAG showed significant differences among OAG, glaucoma suspect, and normal controls and was significantly correlated with functional and structural defects. Retinal nerve fiber layer microcirculation measurement using OMAG may help physicians monitor glaucoma.

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“…Prior to imaging, all patients with ACHM were dilated using either cyclomydril or a combination of tropicamide (1%) and phenylephrine hydrochloride (2.5%) for cycloplegia and pupillary dilation respectively. Control patients were imaged without dilation, as a previous study evaluated layer thickness measurements pre- and post-dilation and found no change 32 . The Bioptigen spectral domain-OCT (Leica Microsystems, Wetzlar, Germany) was used to acquire line scans at the fovea in both eyes of each subject.…”

Section: Methodsmentioning

confidence: 99%

Interocular Symmetry and Repeatability of Foveal Outer Nuclear Layer Thickness in Congenital Achromatopsia

Mastey

Litts

Langlo

et al. 2018

Preprint

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Cone Photoreceptors 18 19Precise: (21/35 Submitted separately as "Highlights" file) We examined the intraobserver 20 repeatability and interocular symmetry of foveal outer nuclear layer (ONL) thickness 21 measurements in patients with congenital achromatopsia. . CC-BY-NC 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/367813 doi: bioRxiv preprint first posted online Jul. 12, 2018; Purpose: To examine the intraobserver repeatability of foveal outer nuclear layer (ONL) 25 thickness measurements and evaluate interocular symmetry for patients with achromatopsia 26 (ACHM) and controls. 27Design: Cross-sectional study. 28 Subjects: Sixty-four patients with CNGA3-or CNGB3-associated ACHM and 38 patients 29 with normal vision were recruited for analysis. 30Methods: Horizontal line scans through the fovea of each eye were acquired using 31 optical coherence tomography. Three foveal ONL thickness measurements were made by a 32 single observer using custom software to analyze repeatability. Interocular symmetry was 33 assessed using the average of the three measurements for each eye.

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Evaluating the effect of pupil dilation on spectral-domain optical coherence tomography measurements and their quality score

Cited by 18 publications

References 19 publications

Assessing the Interocular Symmetry of Foveal Outer Nuclear Layer Thickness in Achromatopsia

Assessing the Interocular Symmetry of Foveal Outer Nuclear Layer Thickness in Achromatopsia

Peripapillary Retinal Nerve Fiber Layer Vascular Microcirculation in Glaucoma Using Optical Coherence Tomography–Based Microangiography

Interocular Symmetry and Repeatability of Foveal Outer Nuclear Layer Thickness in Congenital Achromatopsia

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