Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats

Tanideh, Nader; Bahrani, Maryam; Khoshnood-Mansoorkhani, Mohammad Javad; Mehrabani, Davood; Firoozi, Donya; Koohi-Hosseinabadi, Omid; Iraji, Aida

doi:10.17795/acr-42856

Cited by 11 publications

(16 citation statements)

References 36 publications

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“…16,17 To induce experimental UC, different methods such as administration of trinitrobenzene sulphonic acid (TNBS), 17 dextran sulfate sodium, and acetic acid have been used. 18 Induction of colitis with acetic acid is one of the standard experimental models for creation of UC, which is similar in many histopathological features to UC in humans including infiltration of neutrophils, acute inflammation, injuries in mucosal and submucosal layers, and necrosis in colon tissue. 19 Given that nutrition and diet can affect the patients with inflammatory bowel disease, 20 the use of natural compounds for the prevention and treatment of inflammatory bowel disease shows an increasing trend.…”

Section: Discussionmentioning

confidence: 99%

The Healing Effect of Aloe Vera Gel on Acetic Acid-Induced Ulcerative Colitis in Rat

Hassanshahi

Masoumi

Mehrabani

et al. 2020

Middle East J Dig Dis

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BACKGROUND The use of herbal and synthetic compounds can be effective in improving the areas and repair of tissues that have been affected during the processes like what happens in ulcerative colitis (UC) as a common inflammatory disorder. According to the beneficial effects of aloe vera, in this study, we aimed to assess the therapeutic effects of oral aloe vera gel on acetic acid-induced colitis in rats by histopathological and molecular analysis of Bax, and BCL-2 genes expression (using RT-PCR technique) in colon tissue samples. METHODS This experimental study comprised 32 adult male Sprague Dawley rats weighting 220 ± 20 g that were randomly divided into four groups as follows. The control group (healthy rats), colitis group in which UC was induced by transrectal administration of 3% acetic acid with no treatment, oral form of sulfasalazine group in which UC was induced by transrectal administration of 3% acetic acid, then was treated by oral administration of sulfasalazine 500 mg/kg body weight, and the fourth group which received oral form of aloe vera gel (200 mg / kg) for 21 days, respectively after induction of UC. Then, the therapeutic effects of treatment groups were compared with the control group and the colitis group with no treatment, by the assessment of histopathological and molecular changes in the colon tissues of rats on the 7th, 14th, and 21st days. Finally, the collected data were analyzed using statistical tests. RESULTS Histologically, aloe vera gel treatment could reduce and heal colon tissue damages in induced colitis. Also, this gel reduced apoptosis in rat’s colon with acetic acid-induced colitis, which showed in significantly decreased in Bax mRNA expression and significantly increased BCL-2 mRNA expression compared with the colitis group with no treatment. CONCLUSION Aloe vera gel has a significant effect on the treatment of UC in rat because of the beneficial effect that was found from aloe vera such as decreasing the severity of colitis as evidenced by histopathological findings, and with respect to apoptosis and gene expression that were related to wound healing process, and suppression of the elevation of Bax mRNA with the upregulation of Bcl-2, which can be considered effective in the treatment of UC.

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Section: Discussionmentioning

confidence: 99%

The Healing Effect of Aloe Vera Gel on Acetic Acid-Induced Ulcerative Colitis in Rat

Hassanshahi

Masoumi

Mehrabani

et al. 2020

Middle East J Dig Dis

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“…A solution of 2,4,6-trinitrobenzenesulfonic acid (TNBS, 150 mg/kg) was dissolved in 50% (V/V) ethanol to break the intestinal barrier and was slowly injected into the colon while the control group received only 50% ethanol. Animals were hanged in the air vertically for 40 seconds and then returned to their cages [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

The Antioxidant and Anti-Inflammatory Effects of Quercus brantii Extract on TNBS-Induced Ulcerative Colitis in Rats

Naini

Mehrvarzi

Zargari-Samadnejadi

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objectives. Ulcerative colitis is a common subtype of persistent inflammatory bowel disease with high morbidity consequences. Despite unknown definite pathogenesis, multiple anti-inflammatory medications are used for its treatment. Traditionally, Quercus brantii (QB), mostly available in the Middle East, has been used for gastrointestinal disorders. Other beneficial effects associated with QB include reduction of oxidative stress, inflammations, homeostatic instability, and improvement in clinical conditions. Materials and Methods. This experimental study was designed to assess the possible therapeutic effects of QB on UC and compare its effects with those of sulfasalazine. Of the 70 Wistar rats clustered in seven groups, ten received only alcohols and sixty were confirmed to be suffering from trinitrobenzene sulfonic acid- (TNBS-) induced colitis. Four groups received different dosages of QB extract via oral and rectal routes, one received sulfasalazine, and the other remaining two groups received nothing. The effects of QB were evaluated by assessing macroscopic and histologic scoring, measuring inflammatory mediators, and determining oxidative stress markers. Results. Comparing to the untreated TNBS-induced control groups, QB-treated groups showed a dose- and route-dependent improvement comparable with sulfasalazine. Treating rats with QB reduced the microscopic and macroscopic damage, decreased TNF-α, IL-6, NO, MPO activity, and MDA content, increased superoxide dismutase (SOD) activity, and reduced body weight loss. Conclusions. Our data recommended the anti-inflammatory and antioxidant effects of QB extract in a dose-dependent manner.

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“…The concentration of malondialdehyde was assayed by the method previously described by Ohkawa et al (1979) 39 using a commercial kit (ZellBio, Germany) 25,40 …”

Section: Methodsmentioning

confidence: 99%

Novel strategy of cartilage repairing via application of P. atlantica with stem cells and collagen

et al. 2021

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Osteoarthritis (OA) is an inflammatory joint condition, still lacking effective treatments. Some factors consider as the main causes of OA, including biochemical, mechanical, and genetic factors. The growth of studies confirmed that modern medicine in combination with folk medicine regarding the arrival of reliable, efficient, and safe therapeutic products against OA. In the present study, the effects of various single and combinatorial treatments of knee articular cartilage, including stem cells, collagen, and P. atlantica hydroalcoholic leaves extract were investigated in a rat‐induced OA model. On week 12 after OA confirmation, histopathology and radiography assessments were evaluated and the serum and synovial fluid levels of TAC, TNF‐α, PEG2, MPO, MMP3, MMP13, and MDA were also measured. Combination therapy of OA‐induced rats with hydroalcoholic extract of P. atlantic leaves, stem cells, and collagen considerably increased the efficacy of treatment as evidenced by increasing the TAC and lowering TNF‐α, MPO, MMP3, and MMP13 compared to control group and even groups received single therapy. This is in agreement with a high amount of total phenolic compounds and antioxidant capacities of the hydroalcoholic extract of P. atlantic leaves. It is concluded that multifunctional agents targeting the pathophysiology of OA has exhibited significant therapeutic effects against OA.

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Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats

Cited by 11 publications

References 36 publications

The Healing Effect of Aloe Vera Gel on Acetic Acid-Induced Ulcerative Colitis in Rat

The Healing Effect of Aloe Vera Gel on Acetic Acid-Induced Ulcerative Colitis in Rat

The Antioxidant and Anti-Inflammatory Effects of Quercus brantii Extract on TNBS-Induced Ulcerative Colitis in Rats

Novel strategy of cartilage repairing via application of P. atlantica with stem cells and collagen

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