Evaluating the Content of Active Principles from Wild Hypericum perforatum L. in Various Harvesting Seasons

Ionescu, Elena; Iordache, Tanta‐Verona; Ţebrencu, Carmen Elena; Cretu, Ruxandra; Florea, Ana Mihaela; Radu, Anita Laura; Zaharia, Anamaria; Sârbu, Andrei

doi:10.37358/rc.18.7.6439

Cited by 4 publications

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“…Based on biological functions, the 749 genes were divided into 11 categories: photosynthesis/energy (25), primary metabolism (85), secondary metabolism (62), cell morphogenesis (60), bio-signaling (93), hormone biosynthesis (23), polynucleotide biosynthesis (33), transcription factor (112), translation (58), transport (104) and stress response (94) (Figure 4D). Based on the pathways for the control of Hyp biosynthesis, plant growth and stress response [10,20], finally, 150 DEGs (90 UR and 60 DR) were dug out as master genes involved in photosynthesis (12), carbohydrate metabolism (13), fatty acid metabolism (15), cytochrome P450 (21), morphophysiological traits (15), heat shock protein (15), coldresponsive protein (3) and transcription factors (56). The differential expression levels of the 150 genes based on Reads Per kb per Million (RPKM) values are shown in a heat map (Figure 5 and Tables S4-S10).…”

Section: Biological Category Of Degsmentioning

confidence: 99%

“…Currently, Hyp is mainly extracted from H. perforatum [ 8 ]. In order to improve the Hyp production, many approaches that have been investigated include dissecting the Hyp biosynthetic pathway [ 9 , 10 , 11 ], screening germplasm resources [ 12 , 13 , 14 ], evaluating harvest stages [ 8 , 12 , 15 ], finding out the optimization of environmental factors such as nutrients [ 16 ], drought stress [ 17 ], UV-B radiation [ 18 , 19 , 20 ], light [ 21 ] and temperature [ 12 , 20 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Chen

et al. 2021

Molecules

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Hypericin (Hyp), well-known as an antidepressant, is mainly extracted from Hypericum perforatum. Although Hyp accumulation and biomass are greater at lower compared to higher temperature, the regulation mechanism has not been reported. Here, the physiological characteristics and transcriptome of H. perforatum grown at 15 and 22 °C were determined and analyzed by HPLC and de novo sequencing. The results showed that the stomatal density and opening percentages were 1.1- and 1.4-fold more, and the Hyp content was 4.5-fold greater at 15 °C compared to 22 °C. A total of 1584 differentially expressed genes (DEGs) were observed at 15 versus 22 °C, with 749 characterized genes, 421 upregulated (UR) and 328 downregulated (DR). Based on biological functions, 150 genes were associated with Hyp biosynthesis, plant growth and the stress response, including photosynthesis, carbohydrate metabolism, fatty acids metabolism, cytochrome P450 (CYPs), morpho-physiological traits, heat shock proteins (HSPs), cold-responsive proteins (CRPs) and transcription factors (TFs). The differential expression levels of the master genes were confirmed by qRT-PCR and almost consistent with their Reads Per kb per Million (RPKM) values. This physiological and transcriptomic analyses provided insight into the regulation mechanisms of low temperature enhancing Hyp biosynthesis in H. perforatum.

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Section: Biological Category Of Degsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Chen

et al. 2021

Molecules

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“…Determination of extractible substances ES, of flavones (as rutoside), of polyphenolcarboxylic acids (as caffeic acid) and of naphtodianthrones as hypericin were performed according to protocols described in a previous paper [15] and calculated using equations [1][2][3] and [4], respectively. (1) where ES %= soluble substance percent, m1 and m2 (g) the weight of resulted residue and that of the crude plant product, respectively (2) where Ap= sample absorbance (a.u.…”

Section: Quantitative Determination Of Active Substancesmentioning

confidence: 99%

Development of Single-step Protocols for the Separation of Naphtodianthrones from St. John�s Wort Extracts

Ionescu¹,

Iordache²,

Ţebrencu³

et al. 2018

Rev. Chim.

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“…Another important factor to be studied is the influence of season on the yield and composition of the obtained products. This factor has been studied in other plants, such as seomcho [41], hypericum [42], asparagus [43] and oregano [44], as well as in olive [45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Influence of Drying Temperature and Harvesting Season on Phenolic Content and Antioxidant and Antiproliferative Activities of Olive (Olea europaea) Leaf Extracts

Losada-Echeberría

Naranjo

Malouche

et al. 2022

IJMS

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Interest in plant compounds has increased, given recent evidence regarding their role in human health due to their pleiotropic effects. For example, plant bioactive compounds present in food products, including polyphenols, are associated with preventive effects in various diseases, such as cancer or inflammation. Breast and colorectal cancers are among the most commonly diagnosed cancers globally. Although appreciable advances have been made in treatments, new therapeutic approaches are still needed. Thus, in this study, up to 28 olive leaf extracts were obtained during different seasons and using different drying temperatures. The influence of these conditions on total polyphenolic content (measured using Folin–Ciocalteu assays), antioxidant activity (using Trolox Equivalent Antioxidant Capacity and Ferric Reducing Ability of Plasma assays) and antiproliferative capacity (using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT assays) was tested in breast and colorectal cancer cells. Increased phenolic composition and antioxidant and antiproliferative capacity are noted in the extracts obtained from leaves harvested in autumn, followed by summer, spring and winter. Regarding drying conditions, although there is not a general trend, conditions using the highest temperatures lead to the optimal phenolic content and antioxidant and antiproliferative activities in most cases. These results confirm previously published studies and provide evidence in support of the influence of both harvesting and drying conditions on the biological activity of olive leaf extracts.

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Evaluating the Content of Active Principles from Wild Hypericum perforatum L. in Various Harvesting Seasons

Cited by 4 publications

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Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Development of Single-step Protocols for the Separation of Naphtodianthrones from St. John�s Wort Extracts

Influence of Drying Temperature and Harvesting Season on Phenolic Content and Antioxidant and Antiproliferative Activities of Olive (Olea europaea) Leaf Extracts

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