Evaluating the comparative efficacy of Bioactive glass containing Toothpaste, Amine fluoride-containing toothpaste and CPP-ACPF on remineralization of Artificial Incipient Enamel Lesion

Abstract

Objectives: The purpose of this in-vitro study was to evaluate the comparative efficacy of Bioactive glass containing toothpaste (BioMin) and Amine fluoride-containing toothpaste (Amflor), and CPP-ACPF (GC Tooth mousse plus) on remineralization of artificial incipient enamel lesion and evaluated using Vickers micro-hardness test. Materials and Methods: A total of fifty freshly extracted premolar teeth were collected and decoronated at 1mm below the CEJ. An adhesive tape of size 4x4 mm is placed on the labial surface of each specimen. Following this, the specimens were mounted in acrylic resin moulds such that only the adhesive tape was exposed, and on the removal of the tape, a window of 4x4 mm is created. Then the specimens were randomly divided into five groups, among which specimens of four groups were subjected to demineralization for four days at 370c using an incubator. Group A (sound enamel), Group B (BioMin), Group C (CPP-ACPF), Group D (Amflor), Group E (demineralized lesion). The specimens of groups B,C,D were subjected to pH cycling for 10 days. Following whichmicro-hardness,all the specimens were evaluated using Vickers Micro-hardnessTest. Statistical analysis: The analysis was done using One Way ANOVA and inter-group comparison was done using a post hoc test. Results: Results showed statistically significant differences (p<0.001) between groups B,C,D, and group E. Among the interventional groups even though there was no significant difference, group B( BioMin) showed higher micro-hardness values when compared to group C (CPP-ACPF) and group D(Amf). Conclusion: Under the limitation of this study, it can be concluded that Bioactive glass-based toothpaste (BioMin) is a novel technology that can be used for the remineralization of early enamel caries.