Evaluating the antibacterial effect of cobalt nanoparticles against multi-drug resistant pathogens

Abstract

This study aimed to estimate the effect of cobalt nanoparticles (Co NPs) with different concentrations against multidrug-resistant (MDR) pathogenic bacteria. Three isolates of (gram-positive), . (gram-negative), and (gram-negative) bacteria were extracted from various clinical examples utilizing routine methods on bacteriological culture media. The antibacterial sensitivity of commercial antibiotics such as Ciprofloxacin, Cefotaxime, Gentamycin, and Amoxicillin was broken down on a Muller Hinton agar plate and evaluated using the disk diffusion method. The study results demonstrated the antibacterial effect of the Co NPs against the bacterial isolates with three different concentrations utilized in the study. The results indicated that the Co NPs showed the highest antibacterial activity when utilizing 100 μg/ml against followed by and with zones of inhibition measured as 22.2±0.1 mm, 20.3±0.15 mm, and 15.8±0.1 mm; respectively. Co NPs at a 100 μg/mL concentration showed higher inhibition zones than several common antibiotics except for Ciprofloxacin, which demonstrated better antibacterial activity against the bacterial isolates employed in this study. Scanning Electron Microscope (SEM)and X-Ray diffraction (XRD)studies confirmed that Cobalt nanoparticles (Co NPs) were synthesized from cobalt sulphate solution with a size ranging from 40 nm to 60 nm. The nanoparticles showed a crystalline structure with a round shape and smooth surface. The antibacterial resistance of Co NPs against three common bacteria such as , , and was assessed in this study. The optimum concentration of the Co NPs was identified as 100 μg/ml, which could provide a similar or higher antibacterial effect.

TiO2/ZIF-67 nanocomposites synthesized by the microwave-assisted solvothermal method: a correlation between the synthesis conditions and antimicrobial properties

“…27 In addition, it has low toxicity, as demonstrated by L. Qian et al 14 It is important to highlight that several mechanisms of antimicrobial action have been associated with the release of Co 2+ ions, such as the change in the correction functions of plasma membrane proteins and accumulation in the cell wall, leading to a decrease in its permeability. 10,48 Concerning composites, Qian et al synthesized new ZIF-67 and cellulose-based composites with outstanding mechanical properties and antibacterial activity against E. coli. 14 Recently, Tao et al obtained a composite consisting of osteogenic growth peptide (OGP) loaded zeolitic imidazolate framework-67 (ZIF-67) and coated it on titanium dioxide nanotubes (TNTs) (TNT-ZIF-67@OGP) with strong antibacterial activity against E. coli, S. aureus, Streptococcus mutans (S. mutans), and methicillin-resistant Staphylococcus aureus (MRSA).…”

Study of the antibacterial effects of the starch-based zinc oxide nanoparticles on methicillin resistance <i>Staphylococcus aureus</i> isolates from different clinical specimens of patients from Basrah, Iraq

“…These results were consistent with the previous studies in which higher concentrations of ZnO-NPs showed stronger antimicrobial effects [8] , [25] , [26] . However, some studies showed that the inhibition zone of nanoparticles starts to shrink beyond an optimal concentration [28] , [29] . One of the possible reasons may be due to the accumulation of nanoparticles in high concentrations and the inability to penetrate into bacterial cells [28] , [29] .…”

“…However, some studies showed that the inhibition zone of nanoparticles starts to shrink beyond an optimal concentration [28] , [29] . One of the possible reasons may be due to the accumulation of nanoparticles in high concentrations and the inability to penetrate into bacterial cells [28] , [29] .…”

Bioengineered Bacterial Flagella-Templated in Situ Green Synthesis of Polycrystalline Co3O4 Nanowires for Gram-Negative Antibacterial Applications

“…Cobalt ion has mild toxicity for a healthy individual, but a cobalt complex has been used as an anticancer agent . Meanwhile, recent studies reveal that cobalt nanoparticles , and cobalt-related materials have antibacterial properties. However, the traditional method for the synthesis of cobalt nanowires required high temperatures and many reaction steps. − Therefore, developing a simple technique to synthesize the cobalt-related nanowires at room temperature is essential to produce an efficient antibacterial material.…”

“…Cobalt ion has mild toxicity for a healthy individual, 14 but a cobalt complex has been used as an anticancer agent. 15 Meanwhile, recent studies reveal that cobalt nanoparticles 16,17 and cobalt-related materials 18 have antibacterial properties. However, the traditional method for the synthesis of cobalt nanowires required high temperatures and many reaction steps.…”