Evaluating some biochemical parameters and their correlation withviral load in patients with Hepatitis B virus, Thi-Qar, Iraq

AlaaHameedFazaa,; Kredy, Husam M.; Atya, Ahmed K.

doi:10.36295/asro.2020.232121

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Liver enzymes additionally increase in all liver diseases other than HBV, so these don't seem to be specific for HBV. Anti HBsAg enzyme linked immunosorbent serologic assay shows a sensitivity in the range of 91-97% while PCR is considered to be 100% sensitive and specific for HBV infection 14 .…”

Section: Introductionmentioning

confidence: 99%

Correlation between Hepatic Enzymes and Viral Load of HBV in Hepatitis B patients

Ijaz¹,

Faraz²,

Atif³

et al. 2023

PJMHS

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Background: Hepatitis B virus is a causative agent of hepatitis B, and it is a severe global health issue. Aim: To investigate the increased level of aminotransferases and its association with viral load of HBV infected patients and to find the most sensitive and reliable technique for identification of HBV infection. Methods: This cross-sectional study included 198 patients of HBV. Blood samples were collected from these patients in serum tubes for detection of HBV by different techniques such as Immunochromatographic (ICT), Enzyme linked immunosorbent assay (ELISA), and whole blood was collected which was further processed for DNA extraction and real time PCR. Results: There were 64 female and 134 males in this study. All the patients were positive for HBV by the ICT method and also by ELISA method but for the RT-PCR out of these 198 patients 89(45%) were positive and 109 (55%) were negative. This study demonstrates no significant correlation between liver enzymes with viral load. The ICT test method is active and simple but not a precise and unique test also has no statistical correlation with viral load, but ELISA is correlated with viral load recognized by real time PCR. (P value=˂0.001, 95% Cl=0.40-0.69, r value =0.56). Conclusion: Our results concluded that the immunochromatographic technique is fast and easily available but not a precise and definitive test and has no statistical correlation with viral load, but ELISA is associated with viral load proved by RT-PCR. Real time polymerase chain reaction is the gold standard test for the detection of Hepatitis B virus infection. The practical implication of this study is that ICT technique is no longer a reliable technique for the diagnosis of HBV infection and the patients positive on ICT should be confirmed for HBV in PCR for both diagnostic and prognostic purposes. Keywords: Hepatitis B Virus, Liver enzymes, Immunochromatographic, Viral load, Enzyme linked immunosorbent assay

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Section: Introductionmentioning

confidence: 99%

Correlation between Hepatic Enzymes and Viral Load of HBV in Hepatitis B patients

Ijaz¹,

Faraz²,

Atif³

et al. 2023

PJMHS

View full text Add to dashboard Cite

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Evaluation of Prothrombin Time,International Normalized Ratio, and Activated Partial Thromboplastin Time in Hepatitis B Virus Patients, Sudan2020 -2021

Modawe,

Esra Awad Mohamed,

Ozaz Yagoup Mohamed

et al. 2024

AJPS

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Background: Hepatitis B virus is a growing problem worldwide leading to increasing incidences of life-threatening complications of which liver diseases play a major part of it. Materials and Methods: To evaluate these effects in Sudanese patients, a cross-sectional study was carried out in Wad Medani teaching hospital from October 2020 to February 2021 for assessing the effect of the disease on the coagulation profile. Patients were randomly selected according to inclusion and exclusion criteria. 2.5ml of blood samples were collected from each patient in tri-sodium citrate containers, and then semi-automated Coatron M4 was used to estimate Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) after calibrated and controlled setting according to the manufacturer’s instructions. SPSS (version 25) was used to analysed data. Results: A total of 100 hepatitis B virus patients were included in the study, with males representing (79%) and females were (21%), their age were between 18 to 60 years. The study results showed PT prolonged in (98%) of the study population and only (2%) were normal. regarding the APTT (99%) of them had prolonged results (more than the upper normal), and the remaining (1%) was normal. Also, the study results showed there were slight statistically insignificant differences in coagulation profile in hepatitis B virus patients according to gender, age group, family history, and duration of the disease. Conclusion: hepatitis B infection had a great effect on Coagulation profiles. Therefore, it is recommended on the basis of these results to request PT, INR, and APTT as routine works up for hepatitis B virus patients.

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Evaluating some biochemical parameters and their correlation withviral load in patients with Hepatitis B virus, Thi-Qar, Iraq

Cited by 2 publications

References 28 publications

Correlation between Hepatic Enzymes and Viral Load of HBV in Hepatitis B patients

Correlation between Hepatic Enzymes and Viral Load of HBV in Hepatitis B patients

Evaluation of Prothrombin Time,International Normalized Ratio, and Activated Partial Thromboplastin Time in Hepatitis B Virus Patients, Sudan2020 -2021

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