Evaluating Sheath Blight Resistance in Rice Using Detached Tiller and Field Screening Method

Chaudhary, B. D.

doi:10.3126/jnarc.v1i0.15717

Cited by 4 publications

(3 citation statements)

References 18 publications

(26 reference statements)

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“…We predicted, and confirmed that the US japonica lines CCDR, CL53, Cypress, Blue Bonnet, CL111 carry the Os WAK91 T allele associated with known susceptibility 4, 31, 32–38 . In contrast, the indica lines (IR29, IR64, Kasalath, Jasmin, TeQing, Nagina 22, Pokkali and Nonabokra) were confirmed to carry the resistant C allele (Figure-1G), and exhibit some level of known SB resistance phenotype 35, 39–41 . One exception is 93-11, from which the indica reference genome was sequenced 42 , and which is known to be moderately susceptible 39, 43 .…”

Section: Discussionmentioning

confidence: 95%

Loss of premature stop codon in theWall-Associated Kinase 91(OsWAK91) gene confers sheath blight disease resistance in rice

Al-Bader

Meier

Geniza

et al. 2019

Preprint

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23The genetic arms race between pathogen and host plant is a tug of war that has been ongoing for 24 millennia. The "battles" are those of disruption, restoration of signaling and information 25 transmission on a subcellular level. One such battle occurs between rice an important crop that 26 feeds 50% of the world population and the sheath blight disease (SB) caused by the fungus 27 Rhizoctonia solani. It results in 10 30% global yield loss annually and can reach 50% under 28 severe outbreak. Many Receptor like kinases (RLKs) are recruited as soldiers in these battles. 29 Wall Associated Receptor Kinases (WAKs) a subfamily of receptor-like kinases have been 30 shown to play a role in fungal defense. Here we show that rice gene OsWAK91, present in the 31 major SB resistance QTL region on Chromosome 9 is a key component in defense against rice 32 sheath blight. An SNP mutation C/T separates susceptible variety, Cocodrie (CCDR) from the 33 resistant line MCR010277 (MCR). The resistant allele C results in the stop codon loss that 34 results in 68 amino acids longer C terminus carrying longer protein kinase domain and 35 phosphorylation sites. Our genotype and phenotype analysis of the top 20 individuals of the 36 double haploid SB population shows a strong correlation with the SNP. The susceptible allele 37 appears as a recent introduction found in the japonica subspecies reference genome and a 38 majority of the tropical and temperate japonica lines sequenced by the 3000 rice genome project. 39 Multiple US commercial varieties with japonica background carry the susceptible allele and are 40 known for SB susceptibility. This discovery opens the possibility of introducing resistance 41 alleles into high yielding commercial varieties to reduce yield losses incurred by the disease.42 43 103 point. Expression of known genes from each line, at each time point, was compared in a pairwise 104 fashion against the untreated, Day-0 sample for differential gene expression analysis. For CCDR 105 there were 105, 367 and 377 transcripts from 79, 281 and 320 genes at the Day-0 to Day-1, Day-106 0 to Day-3, and Day-0 to Day-5 comparisons, respectively. Whereas, in the MCR line, there 107 were 148 and 597 transcripts from 119 and 443 genes in the Day-0 to Day-3, and Day-0 to Day-5 108 comparisons, while there were no statistically significant differences in expression observed at 109 Day 0 to 1 (Figure-1B). In the Day-0 to Day-3 comparison, 17 common genes were up-regulated 110 in both the CCDR and MCR lines, and one common gene was down-regulated. Similarly, in the 111 6Day 0 to 5 comparison, 63 common genes in the CCDR and MCR lines were up-regulated, while 112 8 genes were down-regulated ( Figure 1B). In the susceptible CCDR line, there was a larger 113 number of differentially-expressed genes at Day-1, and increasing numberson and Day-3, unlike 114

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Section: Discussionmentioning

confidence: 95%

Loss of premature stop codon in theWall-Associated Kinase 91(OsWAK91) gene confers sheath blight disease resistance in rice

Al-Bader

Meier

Geniza

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In contrast, the indica lines IR29, IR64, Jasmin, TeQing, Pokkali, and Nonabokra, and AUS lines Kasalath and Nagina 22, were confirmed to carry the resistant C allele ( Figure 3 A). These indica and AUS lines exhibit some known SB resistance phenotypes [ 53 , 54 , 59 , 63 , 64 ]. One exception is the rice line 93-11 with the indica reference genome [ 65 ], which is known to be moderately susceptible [ 53 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

Loss of a Premature Stop Codon in the Rice Wall-Associated Kinase 91 (WAK91) Gene Is a Candidate for Improving Leaf Sheath Blight Disease Resistance

Al-Bader,

Meier,

Geniza

et al. 2023

Genes

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Leaf sheath blight disease (SB) of rice caused by the soil-borne fungus Rhizoctonia solani results in 10–30% global yield loss annually and can reach 50% under severe outbreaks. Many disease resistance genes and receptor-like kinases (RLKs) are recruited early on by the host plant to respond to pathogens. Wall-associated receptor kinases (WAKs), a subfamily of receptor-like kinases, have been shown to play a role in fungal defense. The rice gene WAK91 (OsWAK91), co-located in the major SB resistance QTL region on chromosome 9, was identified by us as a candidate in defense against rice sheath blight. An SNP mutation T/C in the WAK91 gene was identified in the susceptible rice variety Cocodrie (CCDR) and the resistant line MCR010277 (MCR). The consequence of the resistant allele C is a stop codon loss, resulting in an open reading frame with extra 62 amino acid carrying a longer protein kinase domain and additional phosphorylation sites. Our genotype and phenotype analysis of the parents CCDR and MCR and the top 20 individuals of the double haploid SB population strongly correlate with the SNP. The susceptible allele T is present in the japonica subspecies and most tropical and temperate japonica lines. Multiple US commercial rice varieties with a japonica background carry the susceptible allele and are known for SB susceptibility. This discovery opens the possibility of introducing resistance alleles into high-yielding commercial varieties to reduce yield losses incurred by the sheath blight disease.

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“…Moreover, Praveen et al (2018) in their investigation, performed screening on 57 germplasms of rice through artificial inoculation under field conditions and found most of the germplasms were highly susceptible, except Orgoja, which was resistant and Gopal Ghosh that was moderately resistant. Despite screening thousands of rice germplasms, only a few rice cultivars offer resistance to ShB that have been reported, viz., Teqing (Pinson et al, 2005), Jasmine 85 (Liu et al, 2009), Tetep (Channamallikarjuna et al, 2010, Pecos (Sharma et al, 2009), Sabitri (Chaudhary, 2016).…”

Section: Screening Of Rice F 3 Population Against Sheath Blight Disea...mentioning

confidence: 99%

Phenotypic Screening of F3 Rice (Oryza sativa L.) Population Resistance Associated with Sheath Blight Disease

Timsina¹,

Thera²,

Naveenkumar³

2022

IJBSM

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The experiment was conducted during July–December, 2019 in the Agricultural Research Farm Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India. One hundred and twenty-two F3 rice populations from the cross made between IC277332 (susceptible parent)×IC277275 (moderately resistant parent) were evaluated against sheath blight disease under field conditions. The area under disease progress curve (AUDPC) values categorized rice population into four groups viz., moderately resistant (24), moderately susceptible (38), susceptible (40), and highly susceptible (20). A moderate resistance level to the disease was identified during the study in 24 lines (19.6%) with a mean Percentage Disease Index (PDI) of 12.22%−36.6%. Furthermore, 38 individuals showed moderate susceptibility with AUDPC values (1012−1446 day-1). The maximum PDI and AUDPC value were 76.11 and 2325.56, and the minimum PDI and AUDPC values were 22.78 and 622.22, respectively. The principal component (PCA) biplot analysis showed 71.62% and 12.05% variation, respectively. Unweighted Pair Group Method of Arithmetic Means (UPGMA) cluster analysis grouped the 122 individuals into two major clusters, A and B and sub-clusters. These findings indicated that no rice line resistant to sheath blight had been identified. However, few population lines exhibited moderate resistance, which can be utilized as donor lines to generate sheath blight-resistant rice cultivars. These findings will provide a solid basis for our future breeding and screening activities at the institution.

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Evaluating Sheath Blight Resistance in Rice Using Detached Tiller and Field Screening Method

Cited by 4 publications

References 18 publications

Loss of premature stop codon in theWall-Associated Kinase 91(OsWAK91) gene confers sheath blight disease resistance in rice

Loss of premature stop codon in theWall-Associated Kinase 91(OsWAK91) gene confers sheath blight disease resistance in rice

Loss of a Premature Stop Codon in the Rice Wall-Associated Kinase 91 (WAK91) Gene Is a Candidate for Improving Leaf Sheath Blight Disease Resistance

Phenotypic Screening of F3 Rice (Oryza sativa L.) Population Resistance Associated with Sheath Blight Disease

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