Evaluating Revascularization and Flap Survival Using Vascular Endothelial Growth Factor in an Irradiated Rat Model

Abstract

This study demonstrates the potential of VEGF pDNA to improve revascularization and flap viability in previously irradiated tissue.

GM‐CSF ameliorates microvascular barrier integrity via pericyte‐derived Ang‐1 in wound healing

“…A mixture of siRNA/PEI/pluronic gel was prepared according to a previous described method . siRNA of 33.3 ng +5.3 µL of PEI was dissolved in 100 µL of 30% pluronic F‐127 gel (Sigma) to obtain a mixture of 100 µL siRNA/in vivo‐jetPEI (Polyplus‐transfection Inc., New York, NY) at N / P ratio of 8.…”

Free flap transplantation using an extracorporeal perfusion device: First three cases

“…Modifications to the technique, like direct recirculation of the venous blood and modifications of the perfusate are likely to improve outcome and reduce risks in the future. Experimentally, application of vascular endothelial growth factor or the use of blood substitute solutions have already been tested with promising results (Chang et al, 2009;Angelos et al, 2011).…”

Volumetric changes in transplanted vascularized fat flaps after ischemic or congestive stresses and their relationship to capillary density in a zucker fatty rat model

“…The first novelty was the rat type used, Zucker‐ fa / fa rats. Superficial epigastric fat flaps have been commonly used in animal studies . Regular weekly measurements of the volume of these flaps (not weight) were planned using a micro focus X‐ray CT system under general anesthesia.…”

“…Superficial epigastric fat flaps have been commonly used in animal studies. 4,[13][14][15][16] Regular weekly measurements of the volume of these flaps (not weight) were planned using a micro focus X-ray CT system under general anesthesia. The volume of these flaps is small in Wister and SD rats (which are frequently used in research), and are, thus, difficult to detect on images.…”

“…Superficial epigastric fat flaps based on the superficial inferior epigastric vessels were harvested through a midline skin incision from the epigastrium to the perineum. 4,[13][14][15][16] The epigastric fat pads were exposed and dissected free from the overlying skin and underlying abdominal wall musculature without damaging their vascular pedicles, which were easily identified. The vascular pedicles were isolated as they branched from the femoral vessels.…”