“…However, the presence of nutrients such as protein and lipids may reduce the yeast's performance (Watanabe, Honda, Kashiwamura, Sasano, & Watanabe, 2009a). Defatted rice bran (DRB) has lower concentrations of fat (<2%) and higher protein (18%), total carbohydrates (60%), and starch (21%) contents (Watanabe et al, 2019). Thus, the hydrolysis of DRB proteins is an option to increase the bioethanol yield considering that several studies (Johnston & McAloon, 2014;Lei, Zheng, Wang, Zhao, & Zhao, 2013;Watanabe et al, 2009b;Yao, Lee, Wang, Moura, & Johnson, 2012) demonstrated that the addition of a protease resulted in an increase in the final bioethanol concentration.…”