Evaluating novel fungal secretomes for efficient saccharification and fermentation of composite sugars derived from hydrolysate and molasses into ethanol

Brar, Kamalpreet Kaur; Agrawal, Dhruv; Chadha, Bhupinder Singh; Lee, Hung

doi:10.1016/j.biortech.2018.11.004

Cited by 32 publications

(9 citation statements)

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“…From different genome projects, it is obvious that filamentous fungi in general contain a great variety of plant biomass-degrading enzymes in their genomes [8,[46][47][48][49]. Furthermore, proteomic studies show that many of these enzymes are secreted when the fungi grow on lignocellulosic substrates [38,[50][51][52][53]. The secreted part of proteins is called the secretome but the secretome obviously changes according to the circumstances under which the fungi grow such as cultivation conditions and availability of nutrients.…”

Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

“…The application of plant biomass for production of various bioproducts in biorefineries using the biochemical route, involves pretreatment of the biomass to open the recalcitrant material and make it accessible for subsequent enzymatic hydrolysis to produce fermentative sugars for further processing [59,60]. The complete hydrolysis of lignocellulosic biomass requires an efficient cocktail of enzymes, which industrially most often are produced by filamentous fungi [38,52]. Still, none of those fungi can naturally secrete efficient cocktails containing all the necessary enzymes with highest activities in an optimal ratio.…”

Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

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Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Lübeck

2022

Microorganisms

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Filamentous fungi are a large and diverse taxonomically group of microorganisms found in all habitats worldwide. They grow as a network of cells called hyphae. Since filamentous fungi live in very diverse habitats, they produce different enzymes to degrade material for their living, for example hydrolytic enzymes to degrade various kinds of biomasses. Moreover, they produce defense proteins (antimicrobial peptides) and proteins for attaching surfaces (hydrophobins). Many of them are easy to cultivate in different known setups (submerged fermentation and solid-state fermentation) and their secretion of proteins and enzymes are often much larger than what is seen from yeast and bacteria. Therefore, filamentous fungi are in many industries the preferred production hosts of different proteins and enzymes. Edible fungi have traditionally been used as food, such as mushrooms or in fermented foods. New trends are to use edible fungi to produce myco-protein enriched foods. This review gives an overview of the different kinds of proteins, enzymes, and peptides produced by the most well-known fungi used as cell factories for different purposes and applications. Moreover, we describe some of the challenges that are important to consider when filamentous fungi are optimized as efficient cell factories.

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Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Lübeck

2022

Microorganisms

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“…A prolonged burn cycle and greater energy extraction from the air/fuel charge in the cylinder are two bene ts of greater timing advance, both of which increase the torque and/or horsepower gures at the ywheel [9]. This would potentially permit smaller throttle openings and reduced fuel delivery during a speci c driving cycle, with the result being improved fuel economy, under the controlled circumstances [22] [23].…”

Section: Introductionmentioning

confidence: 99%

Effect on Performance, Combustion and Emission Characteristics of Spark Ignition Engine Powered by Premium Level Gasohol- Paraffin Blends With Spark Advancement & Spark Retardment

Kumbhar,

Khot

2024

Preprint

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Internal combustion engine emissions from burning fossil fuels, particularly those from petrol engines, are a major factor in change in the climate and the generation of greenhouse gases. For a long time, ethanol has been noticed as a viable alternative to gasoline that might also help to lower pollution levels. In the current experimentation investigations, the engine performance, combustion, and emission characteristics were assessed for the various ethanol-premium gasohol blends with partial addition of alkane’s such as n-pentane, hexane etc. With addition of hexane and n-pentane in premium gasoline and ethanol blends, carbon monoxide (CO) emission decreased by 18% than premium gasohol. The lowest emission were observed for premium gasoline with 40% ethanol and 10% n-pentane. It was observed that addition of hexane and n-pentane hydrocarbons found to be decreased by 15% as compared to premium gasool. Furthermore, with addition of ethanol blending, cylinder pressure decreased. Both unburned hydrocarbon (HC) and Nitrogen oxides (NOx) increased in spark advancement from 21 to 15° bTDC and became minimum for spark timing 21° bTDC. Again, it increased from spark retardment from 21° to 30° bTDC. This was because there was incomplete combustion takes place. But engine emissions decreased with partial addition of alkanes with premium gasoline and 20% ethanol with spark advancement as well as retardment. The brake thermal efficiency of PG + 10% P were found maximum. The oxides of nitrogen (NOx) were found minimum by 25% with addition of ethanol and paraffin in premium gasoline.

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“…The lignocellulosic ethanol production process is divided into three stages: pre-treatment, enzymatic hydrolysis, and fermentation. Pre-treatment of biomass is a critical step in the breakdown of the lignincarbohydrate complex network structures, which increases lignocellulose porosity and cellulose accessibility to enzymes in hydrolysis (Singh and Bishnoi 2012;Brar et al 2019;Pandey et al 2022). The alkaline pre-treatment essentially removes the lignin component from lignocellulosic biomass without affecting the other components, reduces the degree of crystallinity in biomass, and breaks the lignincarbohydrate bonds (Agbor et al 2011; Salehian and Karimi 2013).…”

Section: Introductionmentioning

confidence: 99%

Enhanced ethanol production using hydrophobic resin detoxified Pine forest litter hydrolysate and integrated fermentation process development supplementing molasses

Pandey,

Negi

2023

Environ Sci Pollut Res

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High ethanol demand to make up the targets of the future gradual replacement of gasoline it is essential to look for hybrid technologies using rst generation and second-generation biofuel feedstock's. The current study used a two-step sequential pre-treatment ( rst dilute alkali, then dilute acid) of Pine (Pinus roxburghii) forest litter (PFL), for bioethanol production. Further, the sacchari cation of pre-treated PFL was optimized through response surface method using Box-Behnken Design, wherein 0.558 g/g of reducing sugar was under optimized conditions: 12.5% (w/v) of biomass loading, 10 FPU of enzyme, 0.15% (w/v) of Tween-80 in 48 h. During fermentation using Saccharomyces cerevisiae NCIM 3288 strain, the ethanol titre was 22.51 g/L, which was enhanced to 27.38 g/L after detoxi cation of PFL hydrolysate with hydrophobic resin (XAD-4). Furthermore, the hydrolysate was supplemented with molasses (total initial sugar: 100 g/L), wherein 46.02 ± 2.08 g/L ethanol was produced with 0.482 g/g yield and 1.92 g/l/h productivity. These results, showed that BOLT-ON Technology involving integration of molasses, increases the initial sugar availability leading to enhanced ethanol production, therefore can be compatible in bio-re neries to produce high titer ethanol.

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Evaluating novel fungal secretomes for efficient saccharification and fermentation of composite sugars derived from hydrolysate and molasses into ethanol

Cited by 32 publications

References 32 publications

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Effect on Performance, Combustion and Emission Characteristics of Spark Ignition Engine Powered by Premium Level Gasohol- Paraffin Blends With Spark Advancement & Spark Retardment

Enhanced ethanol production using hydrophobic resin detoxified Pine forest litter hydrolysate and integrated fermentation process development supplementing molasses

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Evaluating novel fungal secretomes for efficient saccharification and fermentation of composite sugars derived from hydrolysate and molasses into ethanol

Cited by 32 publications

References 32 publications

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Effect on Performance, Combustion and Emission Characteristics of Spark Ignition Engine Powered by Premium Level Gasohol- Paraffin Blends With Spark Advancement &amp; Spark Retardment

Enhanced ethanol production using hydrophobic resin detoxified Pine forest litter hydrolysate and integrated fermentation process development supplementing molasses

Contact Info

Product

Resources

About

Effect on Performance, Combustion and Emission Characteristics of Spark Ignition Engine Powered by Premium Level Gasohol- Paraffin Blends With Spark Advancement & Spark Retardment