Evaluating glucose and mannose profiles in Candida species using quantum dots conjugated with Cramoll lectin as fluorescent nanoprobes

Oliveira, Weslley Felix de; Cabrera, Mariana Paola; Santos, Natália R.M.; Napoleão, Thiago Henrique; Paiva, Patrícia Maria Guedes; Neves, Rejane Pereira; Silva, Márcia V.; Santos, Beate S.; Coelho, Luana Cassandra Breitenbach Barroso; Filho, Paulo E. Cabral; Fontes, Adriana; Correia, Maria T.S.

doi:10.1016/j.micres.2019.126330

Cited by 16 publications

(6 citation statements)

References 40 publications

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“…Although the conjugate had a decrease in the fluorescence intensity, it continued highly fluorescent, as shown in the inset of figure 4(B). This optical behavior was similar to the conjugates prepared from MSA-CdTe QDs and Con A, Cramoll, or BmoLL lectins, using the adsorption strategy [23,54,55].…”

Section: μMsupporting

confidence: 62%

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Lima

Oliveira

Silva

et al. 2022

Methods Appl. Fluoresc.

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Quantum dots (QDs) have stood out as nanotools for glycobiology due to their photostability and ability to be combined with lectins. Mannose-binding lectin (MBL) is involved in the innate immune system and plays important roles in the activation of the complement cascade, opsonization, and elimination of apoptotic and microbial cells. Herein, adsorption and covalent coupling strategies were evaluated to conjugate QDs to a recombinant human MBL (rhMBL). The most efficient nanoprobe was selected by evaluating the conjugate ability to label Candida albicans yeasts by flow cytometry. The QDs-rhMBL conjugate obtained by adsorption at pH 6.0 was the most efficient, labeling ca. 100% of cells with the highest median fluorescence intensity. The conjugation was also supported by Fourier transform infrared spectroscopy, zeta potential, and size analyses. C. albicans labeling was calcium-dependent; 12% and <1% of cells were labeled in buffers without calcium and containing EDTA, respectively. The conjugate promoted specific labeling (based on cluster effect) since, after inhibition with mannan, there was a reduction of 80% in cell labeling, which did not occur with methyl-α-D-mannopyranoside monosaccharide. Conjugates maintained colloidal stability, bright fluorescence, and biological activity for at least 8 months. Therefore, QDs-rhMBL conjugates are promising nanotools to elucidate the roles of MBL in biological processes.

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Section: μMsupporting

confidence: 62%

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Lima

Oliveira

Silva

et al. 2022

Methods Appl. Fluoresc.

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“…Some labelling techniques report the recognition of carbohydrates in fungi, bacteria, or cancer cells using traditional methods. It has also been found that labelling is improved using coupling agents such as EDC or sulfo-NHS [51][52][53], although the physicochemical characteristics of the lectin-QD complex have not been fully described. To date, no studies have reported the coupling of a lectin with QD using a microfluidic platform.…”

Section: Discussionmentioning

confidence: 99%

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

et al. 2020

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Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The average particle size (380 nm) and the average zeta potential (−18.51 mV) were determined. The cytotoxicity of the TBL-IE2 and TBL-IE2-QD was assayed on HT-29 colon cancer cells, showing no differences between them (p ≤ 0.05), where the LC50 values were 1.0 × 10−3 and 1.7 × 10−3 mg/mL, respectively. The microfluidic technique allowed control of the coupling between the QD and the protein, substantially improving the labelling process, providing a rapid and efficient method that enabled the traceability of lectins. Future studies will focus on the potential use of the QD-labelled lectin to recognize tumor tissues.

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“…and the environment. The cell wall is involved in biofilm formation and interactions with the host cells [ 156 ]. Hence, any changes in the polysaccharide structural composition of the cell wall will directly affect the pathogen-associated molecular patterns [ 157 ].…”

Section: Biomedical Applications Of Qdsmentioning

confidence: 99%

Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots

et al. 2020

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Quantum dots (QDs) are fluorescent nanocrystals with superb photo-physical properties. Applications of QDs have been exponentially increased during the past decade. They can be employed in several disciplines, including biological, optical, biomedical, engineering, and energy applications. This review highlights the structural composition and distinctive features of QDs, such as resistance to photo-bleaching, wide range of excitations, and size-dependent light emission features. Physical and chemical preparation of QDs have prominent downsides, including high costs, regeneration of hazardous byproducts, and use of external noxious chemicals for capping and stabilization purposes. To eliminate the demerits of these methods, an emphasis on the latest progress of microbial synthesis of QDs by bacteria, yeast, and fungi is introduced. Some of the biomedical applications of QDs are overviewed as well, such as tumor and microRNA detection, drug delivery, photodynamic therapy, and microbial labeling. Challenges facing the microbial fabrication of QDs are discussed with the future prospects to fully maximize the yield of QDs by elucidating the key enzymes intermediating the nucleation and growth of QDs. Exploration of the distribution and mode of action of QDs is required to promote their biomedical applications.

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Evaluating glucose and mannose profiles in Candida species using quantum dots conjugated with Cramoll lectin as fluorescent nanoprobes

Cited by 16 publications

References 40 publications

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots

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