Evaluating genetic relationships in seed impatiens, <i>Impatiens walleriana</i>, using AFLP profiling

Carr, Jason; Korban, Schuyler S.

doi:10.1111/j.1439-0523.2004.01018.x

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…Fluorescent methods present several advantages over AFLP in which radioactively labeled primers are used (Carr and Korban, 2004). It increases scoring accuracy by eliminating uncertainty in fragment sizing and intensity (De Riek et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Identification of Fluorescent AFLP and SSR Markers for Differentiation and Analysis of New Guinea Impatiens

Parks¹,

Moyer²,

Lyerly³

2006

jashs

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ADDITIONAL INDEX WORDS. molecular differentiation, microsatellite, Impatiens hawkeri, fl oral crop, DNA fi ngerprint ABSTRACT. Fluorescent amplifi ed fragment length polymorphism (F-AFLP) and microsatellites (SSRs) were used to evaluate new guinea impatiens (Impatiens hawkeri W. Bull) cultivars. Ninety-fi ve quality-selected polymorphic fragments from 10 F-AFLP+3 primer combinations were used to evaluate 100 cultivars representing a variety of colors, forms, and breeding programs. Jaccard similarities and unweighted pair-group method of the arithmetic average (UP-GMA) clustering formed a dendrogram with three cultivar groups, to a large extent clustering the cultivars by breeder with a high cophenetic correlation coeffi cient. A small insert genomic library was created and 442 kb of new guinea impatiens sequence was screened for repetitive motifs, resulting in 14 microsatellite markers. A subset of 46 cultivars representing fi ve commercial breeding companies and 11 cultivar series was selected for microsatellite analysis. Seven loci were polymorphic, with two to six alleles per locus. Although both methods were equally effective in distinguishing the cultivars from one another, the topologies of the dendrograms for the two methods were different. The topology of the AFLP dendrogram refl ected possible relationships based on cultivar series and breeding company, while the SSR dendrogram did not. The objectives of this research were to develop and validate both F-AFLP and SSR methodologies for new guinea impatiens, identify markers that can be reliably used for fi ngerprinting, and create a database for future cultivar comparisons.

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Section: Discussionmentioning

confidence: 99%

“…Recently, an advancement of AFLP methodology known as fl uorescent AFLP has been used in seed impatiens [Impatiens walleriana Hook. f. (Carr and Korban, 2004)] and orchid [Dendrobium Ag. (Xiang and Hong, 2003)].…”

mentioning

confidence: 99%

Identification of Fluorescent AFLP and SSR Markers for Differentiation and Analysis of New Guinea Impatiens

Parks¹,

Moyer²,

Lyerly³

2006

jashs

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“…It is also difficult to compare results from samples run on different agarose gels, which increases scoring error. The fluorescence-labelled AFLP technology and automated electrophoresis and band readings provide stable profiles and are more time-saving than radioactively-labelled and silver-dyeing methods [13]. The method utilized in this study was efficient in separating large numbers of cultivars and varieties of R. glutinosa.…”

mentioning

confidence: 99%

Genetic Relationships amongRehmannia glutinosaCultivars and Varieties

Li²,

Song³

et al. 2008

Planta Med

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Many cultivars of Rehmannia glutinosa are grown in China for medicinal uses, but detailed agronomic and morphological descriptions are available for only a few. Knowledge of genetic relationships among most of the cultivars is also scanty and poorly documented. Here, cultivars, varieties and some sexually produced seeds of R. GLUTINOSA were raised in the field and studied for morphological diversity including shape, color, edges of leaves, color of anther, cornal and root, as well as yield of the medicinal part of the roots. Random amplified polymorphism DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to determine genetic relationships and ribosome DNA internal transcribed spacer (ITS) sequences were used for analyzing sequence variations and phylogenetic history. The 118 and 1019 polymorphic markers produced by 10 RAPD and 8 AFLP primers discriminated cultivars and varieties satisfactorily. Sixty-eight accessions were clustered in three main groups at 0.69 similarity levels by unweighted pair-group method arithmetic average (UPGMA) cluster analysis using RAPD in combination with AFLP markers. The average polymorphism information content (PIC) and Shannon index were 0.438 and 2.19 in RAPD and 0.476 and 26.68 in AFLP primers, respectively. This indicates that AFLP markers would be more efficient than RAPD for screening large numbers of R. GLUTINOSA accessions. The analysis of ITS sequences indicated that ITS1 - 5.8S-ITS2 of R. GLUTINOSA was informative in its 611 - 614-bp-long sequence and had 106 variable sites. Phylogenetic trees generated based on ITS sequences as well as the dendrogram obtained from two molecular markers identified four accessions: BY3, BY5, BY6 and Wildness6, with great genetic divergence.

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Implication of ITS phylogeny for biogeographic analysis, and comparative study of morphological and molecular interspecies diversity in Indian Impatiens

et al. 2018

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Evaluating genetic relationships in seed impatiens, Impatiens walleriana, using AFLP profiling

Cited by 5 publications

References 34 publications

Identification of Fluorescent AFLP and SSR Markers for Differentiation and Analysis of New Guinea Impatiens

Identification of Fluorescent AFLP and SSR Markers for Differentiation and Analysis of New Guinea Impatiens

Genetic Relationships amongRehmannia glutinosaCultivars and Varieties

Implication of ITS phylogeny for biogeographic analysis, and comparative study of morphological and molecular interspecies diversity in Indian Impatiens

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