Evaluating cathepsin activity and SIRT1 cleavage in experimental osteoarthritis

Abstract

Conclusions: Bovine SF from joints of healthy calves contains active aggrecanases that are capable of cleaving both adult human and immature bovine aggrecan in the IGD and in the CS2 region. Human SF from knees of adult injured patients, has the same ability to cleave in the CS2 region of both immature bovine and adult human aggrecan, but may have less activity against the bovine aggrecan IGD site. The cleavage seen in the IGD and the CS2 region of bovine aggrecan after incubation with JC is a delayed process that may involve additional time for diffusive transport of aggrecanases out of the JC, as well as time needed for induction or activation of the aggrecanases. EVALUATING CATHEPSIN ACTIVITY AND SIRT1 CLEAVAGE IN EXPERIMENTAL OSTEOARTHRITIS