Evaluate IL-10 level and gene polymorphism in β-Thalassemia patients with related to microbial infection and disease outcome

Abstract

Thalassemia is a genetic blood disorder inherited from the parent with unusual production of hemoglobin, infectious complications, immune abnormalities and iron overload with related to organ damage that consider a chief causes of morbidity and mortality. So this study aimed to evaluate the important causative agents and the role of IL-10 gene polymorphism in progressive disease. A Case-control study was performed to 46 Beta-thalassemia patients attended to inherited blood center in AL-Zahraa teaching hospital with group of 30 healthy individual as control. Blood sample was collected from all patients and control. IL-10 level were measured by enzyme-linked immunosorbent assay (ELISA). Singule nucleotide polymorphism detected by ARMS-PCR technique. The result shown that 46(46% ) patients with thalassemia , the male higher than female with frequency (65%), according to infection 32(70%) were TTI with HCV a most common pathogen 19(41 %).This study explain that serum level of IL-10 is significantly higher among patients with thalassemia (98±27.53)pg/ml compared to healthy control (10.67±1.49) pg\ml and was higher in HCV infected patients (121.56 ±32.60)pg/ml than non-infected with HCV record ( 73.61± 18.29) pg/ml.