Evaluate effects of the dilution medium and holding time on various motility parameters of delta smelt semen

Rahman, Md. Moshiur; Aghbolaghi, Marzieh Asadi; Hung, Tien‐Chieh

doi:10.1016/j.theriogenology.2022.11.029

Cited by 1 publication

(1 citation statement)

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“…The experimental crosses were made by manually expressing and combining gametes from the fish that were selected for each cross (Ellison et al 2023). For the pooled crosses, milt from three males was admixed in an extender vial (Rahman et al 2023) and then added into a container with premixed eggs from three females (Figure 1A). For the partial-factorial crosses, eggs from each of three females were apportioned among three containers and admixed with eggs from two other dams prior to the fertilization step; each container then received milt from a single male (Figure 1B).…”

Section: Experimental Crossesmentioning

confidence: 99%

Assessing captive spawning strategies for supplementation production of Delta Smelt

LaCava,

Donohue,

Badger

et al. 2023

Trans Am Fish Soc

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To support the declining wild population of Delta Smelt, a conservation hatchery has expanded its mission from maintaining a backup population as insurance against extinction to also producing fish for release into the wild. The substantially higher production demands require a balance between producing large numbers of fish while adhering to conservation genetic principles that maximize retention of effective population size (Ne) and thus overall diversity. We performed spawning experiments at the hatchery to evaluate the genetic consequences of two spawning strategies: 1) a pooled strategy where we fertilized premixed eggs from three dams with premixed milt from three sires and 2) a partial‐factorial strategy where eggs from three dams were mixed and then apportioned among three containers; each container then received milt from one sire. We used genetic parentage analysis of larval offspring to determine the reproductive success of spawners in ten replicate crosses of each strategy. Contributions of parents to offspring were more even in partial‐factorial crosses and consequently resulted in higher Ne (average Ne = 5.50 ± 0.38; expected Ne = 6.0), suggesting its potential for maintaining genetic diversity over time. In contrast, our pooled spawning experiment produced lower and more variable Ne values (average Ne = 3.86 ± 1.30), demonstrating that this more efficient method of production entails high costs in terms of long‐term genetic management. Treating our experiments as hypothetical pools of fish for release, we combined Ne values for pooled or partial‐factorial crosses to calculate the effective size of a release population (NeR). Unequal family sizes reduced the NeR for our pooled experiment to half of the expected value, whereas the partial‐factorial experiment NeR was 88% of the expected value. We discuss the benefits and risks of each method and how these can be considered when designing a spawning strategy for Delta Smelt supplementation.

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Section: Experimental Crossesmentioning

confidence: 99%