Evaluasi Tiga Prosedur Penyiapan Sampel Daging untuk Deteksi Penyakit Mulut dan Kuku dengan Uji RT-PCR

Abstract

Foot and Mouth Disease is strategic contagious animal disease and still excotic in Indonesia. The increase of meat demands resulting the importation of meat products from FMD infected country. To anticipate the emergence of this disease, a quick and accurate diagnostic test is required. RT-PCR is a diagnostic test which is recommended by World Animal Health Organization (OIE). However, there is lack of information on the meat preparation procedures for the detection of the disease by RT-PCR. The aim of this study was to evaluate three meat prepration procedures for the detection of FMD by RT-PCR. Meat samples were immersed in synthetic positive control with concentration of 1:1,000 and homogenized by using mortar and pestle, micropestle and syringe. Processed sampels were extracted and tested with RT-PCR. Results indicated that meat samples homogenized by mortar and pestle showing positive results while samples homogenized with micropestle and syringe were negative. This suggests that homogenizaton with mortar and pestle can be used to prepare meat samples for the detection of FMD by using RT-PCR.

Avian Influenza-H5N1 dan Newcastle Diseases pada Ayam Petelur

“…Uji Hemaglutinasi (HA) dilakukan untuk mendeteksi keberadaan virus AI dan ND. Hemaglutinasi merupakan proses penggumpalan sel darah merah yang terlihat seperti butir-butir pasir (Nuradji et al, 2008). Virus AI dan ND merupakan virus yang memiliki protein Hemaglutinin (HA) pada amplopnya.…”