Evaluación pre-analítica de dos métodos de extracción de ADN para la amplificación del gen de la pneumolisina (PLY) de Streptococcus pneumoniae, en muestras de hemocultivo

Abstract

Background: Streptococcus pneumoniae is a common etiologic agent of invasive respiratory infections among children under (Rev Méd Chile 2004; 132: 533-8).

New Developments in PCR-Based Diagnostics for Bacterial Pathogens Causing Gastrointestinal Infections—A Narrative Mini-Review on Challenges in the Tropics

“…In case of diarrhea due to enteroinvasive bacteria like Campylobacter spp., Salmonella spp., or Shigella spp./enteroinvasive E. coli , not only stool samples but also incubated blood culture material may be considered for PCR-based analysis for diagnostic purposes or in tropical study settings [ 50 ]. In scientific literature, it is a rarely addressed topic that nucleic acid extraction from incubated blood culture materials with various standard procedures including column-based and automatic approaches leads to sample inhibition, making the resulting eluates unsuitable for PCR analysis [ 50 , 51 , 52 ]. It is possible to circumvent this problem by centrifugation-based protocols [ 50 , 51 , 52 ].…”

“…In scientific literature, it is a rarely addressed topic that nucleic acid extraction from incubated blood culture materials with various standard procedures including column-based and automatic approaches leads to sample inhibition, making the resulting eluates unsuitable for PCR analysis [ 50 , 51 , 52 ]. It is possible to circumvent this problem by centrifugation-based protocols [ 50 , 51 , 52 ]. However, those approaches are pretty time-consuming and laborious, so they are hardly suitable for routine diagnostic use in times of MALDI-TOF-MS (matrix-assisted laser-desorption ionization time-of-flight mass spectrometry) application for species identification from incubated blood culture materials [ 53 , 54 , 55 ].…”