Evaluación in vitro de la actividad anti Trypanosoma cruzi de aceites esenciales de diez plantas medicinales

Rojas, Juan Sebastián; Solís, Hilda; Palacios, Olga

doi:10.15381/anales.v71i3.89

Cited by 12 publications

(5 citation statements)

References 27 publications

(29 reference statements)

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“…Pharmacological studies have demonstrated several properties of the essential oil of Cymbopogon citratus (EOCc), such as: antifungal [11,12]; antibacterial [13,14]; anxiolytic, sedative, anticonvulsant [15][16][17], anti-Trypanosoma cruzi [18]; hypotension associated with bradycardia in non-anesthetized normotensive rats and vasodilator in rat mesenteric artery [19]; also, the main constituent of the EOCc, citral, showed significant anti-inflammatory effect due to the inhibition of nitric oxide production through the suppression of NFkB activity and, on lipopolysaccharide activated cells induced hypoexpression of COX-2 (Cyclooxygenase 2) and TNF-α (Tumor necrosis factor-α) [18]. On the other hand, main phytochemical components found in EOCc were neral, citral or geranial, camphene, nonan-4-ol, 6-metil-hept-5-en-2-one, and citronelal.…”

Section: Introductionmentioning

confidence: 99%

The Essential Oil of Cymbopogon citratus Stapt and Carvacrol: An Approach of the Antitumor Effect on 7,12-Dimethylbenz-[α]-anthracene (DMBA)-Induced Breast Cancer in Female Rats

et al. 2020

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C. citratus essential oil and carvacrol have shown an antitumor effect on breast tumor cell lines; the main objective of this research was to evaluate the antitumor effect of the essential oil of Cymbopogon citratus (EOCc) and carvacrol on 7,12-dimethylbenz [a] anthracene (DMBA)-induced breast cancer in female rats. Cancer was induced by a single administration of DMBA at dose of 80 mg/kg body weight (BW). A total of 54 female Holtzman rats were randomly assigned into 9 groups (n = 6). Group I: PS (Physiological saline); Group II: DMBA; Groups III, IV, and V: DMBA + EOCc at doses of 50, 100 and 200 mg/kg/day BW, respectively; Groups VI, VII, and VIII: DMBA + carvacrol at doses of 50, 100 and 200 mg/kg/day BW, respectively; and group IX: DMBA + EOCc + carvacrol at doses of 100 mg/kg/day BW. The treatment lasted 14 weeks. As results, EOCc showed a reduction in tumors as well as necrosis and mitosis. Animals treated with carvacrol did not show necrosis, mitosis, or infiltration. Carvacrol at dose of 100 mg/kg/day BW revealed a significant decrease in the cumulative tumor volume down to 0.11 ± 0.05 cm3 compared to 0.38 ± 0.04 cm3 of the DMBA group (p < 0.01). It is concluded that EOCc and carvacrol had an antitumor effect on DMBA-induced breast cancer in female rats.

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Section: Introductionmentioning

confidence: 99%

The Essential Oil of Cymbopogon citratus Stapt and Carvacrol: An Approach of the Antitumor Effect on 7,12-Dimethylbenz-[α]-anthracene (DMBA)-Induced Breast Cancer in Female Rats

et al. 2020

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“…Of these, EOs from Cymbopogon citratus and Aloysia triphylla were the most active against epimastigote forms, inhibiting T. cruzi growth with IC 50 values of 63.09 and 96.49 μ g/mL, respectively. There was no significant variation in the concentration of nitric oxide, and no cytotoxicity was evident [57].…”

Section: Introductionmentioning

confidence: 99%

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Luna

Scotti

et al. 2019

Oxidative Medicine and Cellular Longevity

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The termneglected diseasesrefers to a group of infections caused by various classes of pathogens, including protozoa, viruses, bacteria, and helminths, most often affecting impoverished populations without adequate sanitation living in close contact with infectious vectors and domestic animals. The fact that these diseases were historically not considered priorities for pharmaceutical companies made the available treatments options obsolete, precarious, outdated, and in some cases nonexistent. The use of plants for medicinal, religious, and cosmetic purposes has a history dating back to the emergence of humanity. One of the principal fractions of chemical substances found in plants are essential oils (EOs). EOs consist of a mixture of volatile and hydrophobic secondary metabolites with marked odors, composed primarily of terpenes and phenylpropanoids. They have great commercial value and were widely used in traditional medicine, by phytotherapy practitioners, and in public health services for the treatment of several conditions, including neglected diseases. In addition to the recognized cytoprotective and antioxidative activities of many of these compounds, larvicidal, insecticidal, and antiparasitic activities have been associated with the induction of oxidative stress in parasites, increasing levels of nitric oxide in the infected host, reducing parasite resistance to reactive oxygen species, and increasing lipid peroxidation, ultimately leading to serious damage to cell membranes. The hydrophobicity of these compounds also allows them to cross the membranes of parasites as well as the blood-brain barrier, collaborating in combat at the second stage of several of these infections. Based on these considerations, the aim of this review was to present an update of the potential of EOs, their fractions, and their chemical constituents, against some neglected diseases, including American and African trypanosomiasis, leishmaniasis, and arboviruses, specially dengue.

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“…Se ha demostrado también que previo a la alteración cardiaca, los ratones infectados presentan infiltración inflamatoria renal que causa daño renal agudo debido a una lesión por isquemia/reperfusión provocado por citocinas proinflamatorias y altas concentraciones de óxido nítrico que, si bien lo protege de la invasión del parásito, causa daño de la integridad y viabilidad celular renal (25) . Existe la posibilidad que esto esté relacionado con la significativa pérdida de peso corporal que se observó en los grupos (figura 4) y que fue mayor en los grupos tratados con aceite esencial de cedrón en dosis de 250 mg/kg, pero al observarse similar efecto en el grupo no infectado y tratado con la misma dosis de cedrón, se podría especular que el aceite de cedrón podría cumplir algún papel en la toxicidad, lo cual podría estar relacionada con la alta dosis utilizada, ya que en el ensayo con células murinas no mostró citotoxicidad (14) , por lo que sería necesario realizar estudios de toxicidad aguda y crónica para establecer la seguridad de su potencial uso como agente anti-Trypanosoma cruzi.…”

Section: Discussionunclassified

“…Ante esta situación, es urgente la búsqueda de nuevos agentes terapéuticos donde las plantas medicinales son una opción viable; no obstante, la inmensa mayoría de estudios de la actividad tripanocida con derivados de plantas, se han realizado solamente por medio de ensayos in vitro. En un estudio recientemente publicado, hemos demostrado que el aceite esencial de A. triphylla inhibió el crecimiento in vitro de epimastigotes de T. cruzi con una moderada CI 50 de 96,5 μg/mL (14) . Esto nos motivó a continuar el estudio, por lo que nos propusimos evaluar in vivo el efecto anti-Trypanosoma cruzi del aceite esencial de A. triphylla en un modelo de ratón.…”

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Efecto del aceite esencial de Aloysia triphylla britton (cedrón) sobre el Trypanosoma cruzi en ratones

Rojas

Palacios

Ronceros

2014

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Determinar la actividad anti-Trypanosoma cruzi in vivo del aceite esencial de Aloysia triphylla en ratones. Materiales y Métodos. Los animales fueron asignados aleatoriamente a los siguientes grupos (n = 15 por grupo): infectados y no tratados (G1), infectados y tratados con benznidazol 100 mg/kg (G2), infectados y tratados con aceite esencial de Aloysia triphylla 100 mg/kg (G3), infectados y tratados con aceite esencial de Aloysia triphylla 250 mg/kg (G4); no infectados y no tratados (G5), y no infectados y tratados con 250 mg/kg de Aloysia triphyla (G6). La infección con T. cruzi se realizó con 10 4 tripomastigotes sanguíneos y el tratamiento empezó en el octavo día postinfección (dpi) hasta el 28 dpi. La parasitemia se determinó con microscopía óptica cada dos días en 5 µL de sangre extraída de la cola. En el 14, 21 y 28 dpi se obtuvo sangre de la cola para el ensayo de creatina kinasa-MB (CK-MB), alanina aminotransferasa y creatinina; después, los animales fueron sacrificados y se extrajo el corazón para el estudio histopatológico. Resultados. El aceite esencial de cedrón produjo una reducción significativa de 85,4% del pico de parasitemia con la dosis de 250 mg/kg; también produjo reducción del número de amastigotes e infiltrados inflamatorios en el corazón. El nivel plasmático de CK-MB también disminuyó en el 28 dpi por efecto de dicho tratamiento. Conclusiones. En condiciones experimentales, el aceite esencial de Aloysia triphylla tiene efecto anti-Trypanosoma cruzi in vivo en ratones. Palabras clave: Trypanosoma cruzi; Cedrón; Aceites esenciales; Experimentación animal (fuente: DeCS BIREME). THE EFFECT OF THE ESSENTIAL OIL FROM Aloysia triphylla BRITTON (LEMON VERBENA) ON Trypanosoma cruzi IN MICE ABSTRACTObjectives. To determine the in-vivo anti-Trypanosoma cruzi activity of the essential oil from Aloysia triphylla in mice. Materials and methods. The mice (n = 15) in the study were randomly assigned to the following groups: infected and untreated (G1), infected and treated with benznidazole 100 mg/kg (G2), infected and treated with of Aloysia triphylla essential oil 100 mg/kg (G3), infected and treated with of Aloysia triphylla essential oil 250 mg/kg (G4), uninfected and untreated (G5), and uninfected and treated with 250 mg/kg of Aloysia triphyla (G6). The infection was conducted using 104 blood T. cruzi trypomastigotes and the treatment began on the 8th day post-infection (dpi) through 28 dpi. The parasitemia was determined through optical microscopy every 2 days using 5 µL of blood sampled from the tail. In dpi 14, 21 and 28, the blood sample was obtained from the queue for testing creatine kinase-MB, alanine aminotransferase and creatinine, then animals were euthanized and the heart was removed for histopathological study. Results. The essential oil of lemon verbena produced a significant reduction of the parasitemia, 85.4% with the peak at a the dose of 250 mg/ kg. It was also observed a reduction in the number of amastigotes and inflammatory infiltrates in the heart. The creatin...

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Evaluación in vitro de la actividad anti Trypanosoma cruzi de aceites esenciales de diez plantas medicinales

Cited by 12 publications

References 27 publications

The Essential Oil of Cymbopogon citratus Stapt and Carvacrol: An Approach of the Antitumor Effect on 7,12-Dimethylbenz-[α]-anthracene (DMBA)-Induced Breast Cancer in Female Rats

The Essential Oil of Cymbopogon citratus Stapt and Carvacrol: An Approach of the Antitumor Effect on 7,12-Dimethylbenz-[α]-anthracene (DMBA)-Induced Breast Cancer in Female Rats

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Efecto del aceite esencial de Aloysia triphylla britton (cedrón) sobre el Trypanosoma cruzi en ratones

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