Evaluación de las técnicas de detección del VPH en los programas de cribado para cáncer de cuello uterino

Abstract

Objective: Detection of high-risk human papillomavirus types (HPV) infection is an important tool in the screening of cervical cancer and triage of cytological abnormalities. The different techniques for detection of this cancer need to be contrasted and validated for use in population screening. Material and Methods: Cervical cell samples were collected from 166 women attending a dermatology clinic in Oviedo (Spain). We evaluated the performance of three different assays for VPH detection. The methods utilized were 1) In-house PCR-EIA using L1 consensus primers MY09/ MY11, 2) A PCR-reverse line blot hybridization (PCR-LBH) that uses L1 consensus PGMY primers. 3) Hybrid Capture 2. All assays were performed blinded. The kappa statistic was used to test for global agreement between assay pairs. Results: HPV DNA was detected in 24,7%, 25,3% and 29,5% of the women, respective to the assay. The overall agreement between the in-house PCR, PCR-LBH and HC2 was (73.5%) with all kappa values between assay pairs exceeding 0.56 (p<0.001). Conclusion: The three HPV assays were equally accurate in estimating high-risk HPV prevalence and HPV-related lesions. The method for HPV detection must be decided depending on the goals of the search (screening, follow-up or molecular studies).

High-risk human papillomavirus infection and associated factors in the anal canal of HIV-positive patients in Medellín, 2017–2018

“…The diagnosis exams employed in the different analyzed studies were based on PCR, a high-sensitivity and specificity test for detection of the virus 34 , but the commercial kits used vary on their ability to identify the viral genotypes. This can lead to minimal differences in prevalence, especially regarding multiple types infections 28 , 29 .…”

Decrease in Sperm Quality due to Infection of Human Papilloma Virus and Chlamydia trachomatis

“…Different techniques have been used in the diagnosis of HVP: in situ hybridization, dot blot, hybrid capture, real-time PCR, ELISA peptides, fused E6/E7 [21,22,23]; these methods have allowed a more accurate and timely diagnosis. The description of existing diagnostic methods is not discussed here, but it is important to mention that each has its own advantages and disadvantages -new technologies have become more accurate in diagnosis.…”

Métodos utilizados en la identificación del virus de papiloma humano

“…En la actualidad la citología cervical es la herramienta más usada para el cribado del cáncer cervical 4 . No obstante, presenta un porcentaje de falsos negativos que, dependiendo del laboratorio, puede alcanzar 20-30% de los frotis examinados 5 .…”

“…Tales observaciones en el diagnóstico ASCUS y las implicaciones clínicas de la infección con VPH de alto riesgo oncogénico (especialmente el tipo 16) ha determinado que investigadores consideren y sugieran la detección directa del VPH en estos casos mediante pruebas moleculares, tomando en cuenta que tal identificación puede ser importante en la estratificación del riesgo de progresión hacia lesiones más graves, para un monitoreo y seguimiento apropiado de pacientes ASCUS. También se ha propuesto introducir la determinación viral con procedimientos de automuestreo como técnica de cribado primario en países subdesarrollados, donde los programas de citología son deficitarios 1,13 .…”