Evaluación de la viabilidad de embriones de cachama blanca (Piaractus brachypomus) conservados a -14 °C en diferentes estadios de desarrollo embrionario

Castillo-Losada, E; Cruz‐Casallas, Pablo E.; Medina‐Robles, Víctor M.

doi:10.4067/s0301-732x2016000100009

Cited by 3 publications

(2 citation statements)

References 26 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fertility rate was recorded 6 h after incubation at the blastula stage. For this purpose, three subsamples of 50 eggs per incubator were randomly taken with a glass rod and classified as viable (translucent) or nonviable (partially or totally white) embryos, as was previously established in our laboratory (Torres‐Tab ares et al 2012; Castillo‐Losada et al 2016). In parallel, fertility was verified by observations on a stereomicroscope (Nikon SMZ 800, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

et al. 2021

View full text Add to dashboard Cite

The Orinoco Striped Catfish Pseudoplatystoma orinocoense is a highly valued commercial‐capture species in Colombia. Although this species was considered endangered and is now in a recovery situation, there are still no cryopreservation studies for its milt. Therefore, the objective of this study was to evaluate the effects of different cryopreservation media and two packaging systems on the sperm motility and fertilization capacity of Striped Catfish. A first experiment was conducted to test the effects of two permeating cryoprotectants (10% dimethyl sulfoxide and 12% methanol) combined with glucose at two concentrations (5.5% and 10%) and with two membrane stabilizers (12% egg yolk and 5% whole milk powder) on the post‐thaw motility of milt samples that were packed into 0.5‐mL straws. The cryopreservation media with the best results in the first experiment were selected for a second experiment in which we evaluated 5‐mL macrotubes as a packaging system. In this case, the response variables were sperm motility and fertility rate. In both experiments, milt with sperm motility that exceeded 90% was diluted at a 1:6 (volume basis) ratio in each of the two cryopreservation media. The diluted samples were packed, equilibrated (for 10 min), then frozen in a nitrogen‐vapor dry shipper for 30 min, and finally stored in liquid nitrogen until they were evaluated. Each factor and its interactions showed a significant effect (P < 0.001) on post‐thaw sperm motility in experiment 1. The best protection during cryopreservation was obtained when 12% methanol supplemented with 5.5% glucose was used as a permeating cryoprotectant, yielding an average motility of 33.3 ± 5.1% (mean ± SD) and 64 ± 5.4% in the 0.5‐ and 5‐mL packaging systems, respectively. Similarly, the highest fertility rate (85%) was recorded under this treatment, with no significant difference (P > 0.05) from the fresh milt. Therefore, Striped Catfish milt can be cryopreserved in 5‐mL macrotubes by using 12% methanol and 5.5% glucose, without the addition of membrane stabilizers.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Ante la ausencia de estímulos ambientales finales en cautiverio para la ovulación y el desove en los peces, uno de los métodos para superar esta disfunción reproductiva es la hipofización (Atencio, 2001), siendo el extracto pituitario de carpa (EPC) uno de los inductores más utilizados (Yaron et al, 2009). Existen registros de reproducción de otros Characiformes con importancia comercial con EPC como bocachico (Atencio-García et al, 2013), cachama blanca (Díaz-Olarte et al, 2010;Castillo-Losada et al, 2016), cachama negra (Arias Acuña y Hernández Rangel, 2009), dorada (Atencio-García et al, 2017), charúa (Atencio-García et al, 2006;Montes y Salgado 2014), comelón (Atencio-García et al, 2001), picuda Salminus affinis (Weingartner y Zaniboni-Filho, 2010;Zanandrea et al, 2016), sábalo Prochilodus lineatus (Botta et al, 2010); lo que señala la efectividad del EPC al permitir la reproducción inducida de especies nativas migratorias que no desovan en cautiverio. Son escasos los reportes en la literatura evaluando la reproducción inducida en especies de la familia Curimatidae; por lo que el objetivo del presente estudio fue evaluar la reproducción inducida de vizcaína Curimata mivartii con EPC.…”

Section: Introductionunclassified

Reproducción en cautiverio de vizcaína Curimata mivartii con extracto pituitario de carpa

Montes-Petro¹,

Atencio-García²,

Estrada-Posada³

et al. 2019

Orinoquia

View full text Add to dashboard Cite

Con el objetivo de reproducir vizcaína Curimata mivartii, ejemplares de dos años de edad (n= 54), mantenidos en estanques en tierra (0,1 pez/m2) fueron inducidos con extracto pituitario de carpa (EPC) (Argent, USA) a razón de 6 mg/Kg de peso para las hembras en dos aplicaciones (10 % y 90% con 12 horas entre aplicación); mientras que los machos fueron inducidos con 4,8 mg/Kg EPC en dosis única. Antes de la inducción hormonal a cada hembra le fue realizada biopsia para verificar su estado de maduración gonadal. La muestra de ovocitos fue tratada con solución Serra (Chemí, Colombia) y después de tres minutos, con la ayuda de un estereoscopio de luz, (Leica, EZ4 W, Alemania) en objetivo de 4x, se determinó posición de la vesícula germinal (céntrico, migrando, periférico, sin núcleo). El análisis de la biopsia ovárica mostró que las hembras se encontraban en maduración final. Los machos inducidos se encontraron en fase de espermiación; los cuales a ligera presión abdominal liberaron líquido seminal. Se estimó el índice de ovulación, fecundidad (absoluta y relativa), diámetro de los ovocitos y volumen seminal. El porcentaje de fertilidad fue estimado a las cuatro horas post-eclosión (hpe) y el porcentaje de eclosión fue estimado a las 10 hpe. Las hembras ovularon entre 5 y 6 horas (28±1,0°C) e inmediatamente los huevos fueron obtenidos por extrusión, así como el semen. La fecundidad absoluta fue de 178331,6±28773,7 ovocitos/hembra y la relativa 137,7±19,6 g ovocitos/Kg de hembra. El diámetro de los ovocitos recién desovados fue de 687,2±42,8 μm. La fertilidad fue de 90,7±1,4% y la eclosión de 85,3±1,5%. Los resultados permiten concluir que EPC a dosificación de 6mg/Kg de peso es un buen inductor de la reproducción de vizcaína, con alto índice de ovulación y altos porcentajes de fertilidad y eclosión.

show abstract

CRYOPRESERVED SEMEN OF <i>Piaractus orinoquensis</i> (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

Medina-Robles,

Suárez-Martínez,

Baldisserotto

et al. 2023

Acta biol. Colomb.

View full text Add to dashboard Cite

Piaractus orinoquensis is a native species from the Colombian Orinoquia and several studies have contributed to the standardization of protocols for seminal cryopreservation of this species. The main goal of this study was to evaluate the sperm motility, duration of sperm motility (DM), pH, sperm membrane integrity (SMI) and fertility of semen cryopreserved for seven years and subjected to different post-thaw storage times (PST). Semen from five males (3.04 ± 0.3 kg) was diluted 1:4 (semen:diluent) with 10 % dimethylsulfoxide, 5.5 % glucose and 12 % egg yolk in 4 mL macrotubes and frozen in liquid nitrogen. Four PST were evaluated: 0, 15, 45 and 60 min. The sperm cells were activated with 1 % NaHCO3 (SB) and 0.9 % NaCl (SC) to determine sperm motility, DM, fertility rate, SMI and pH through PST. Significative motility reduction (p < .05) was observed through the PST and between the PST of 0 and 60 min for DM, both for treatments activated with SB and with SC. The SMI had a significant reduction (p < .05) after 60 min of post-thaw storage and the pH did not vary during PST. The fertility rate decreased significantly (p < .05) between time 0 and 60 min of PST. PST affects the seminal quality and fertility of P. orinoquensis, the best fertilization results were obtained by activating post-thawing sperm motility with 1 % SB. The long-term cryopreserved semen (seven years) of the species maintains its fertilization capacity with values like those obtained with fresh sperm.

show abstract

Evaluación de la viabilidad de embriones de cachama blanca (Piaractus brachypomus) conservados a -14 °C en diferentes estadios de desarrollo embrionario

Cited by 3 publications

References 26 publications

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

Reproducción en cautiverio de vizcaína Curimata mivartii con extracto pituitario de carpa

CRYOPRESERVED SEMEN OF <i>Piaractus orinoquensis</i> (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

Contact Info

Product

Resources

About