EVALUACIÓN DE LA TOXICIDAD DEL CLORPIRIFÓS EN ALEVINOS DE CACHAMA BLANCA (Piaractus brachypomus)

Holguín-Céspedes, Gisella Karina; Ocampo, Laura Marcela Millán; Méndez, Eily Jisseth Mahecha; Rubio, Ángel Enrique Céspedes; Barragán, Iang Schroniltgen Rondón

doi:10.20937/rica.2019.35.04.04

Cited by 7 publications

(1 citation statement)

References 68 publications

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“…The animals were divided into two experimental groups: a treatment group ( n = 5) exposed to a sublethal concentration of CPF (0.011 μ g/L) [ 29 ] for 72 hours and a control group ( n = 3) without CPF exposure. To maintain CPF concentrations in the water, half of the water volume in each glass tank was replaced every 24 hours and the corresponding CPF amount was added.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of High Mobility Group Box 1a (HMGB1a) Gene in Red‐Bellied Pacu, Piaractus brachypomus

Carrillo-Godoy

Rondón‐Barragán

2023

Veterinary Medicine International

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High mobility group box 1 (HMGB1) is a chromosomal protein in the nucleus and a potent extracellular proinflammatory cytokine, widely described in mammals, nevertheless, with scarce reports in fish. In this study, full open reading frame of HMGB1a gene from Piaractus brachypomus is reported as well as its molecular characterization, including tissue gene expression. At predicted protein level, HMGB1a showed similarities with its orthologs in teleosts and higher vertebrates. The relative gene expression of HMGB1a mRNA was measured in several tissues including the brain, where a differential expression appeared in brain regions, i.e., higher expression in the cerebellum and telencephalon. In addition, in an assay of sublethal exposure to chlorpyrifos, upregulation of HMGB1a was detected in optic chiasm. Furthermore, in a traumatic brain injury model, upregulation of HMGB1a expression was evident 24 hours after lesion and remained higher up to 14 days. These findings suggest a role for HMGB1a in brain damage and its candidature as biomarker of brain injury; however, more studies are required to elucidate the functions of HMGB1a and its regulation in P. brachypomus.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of High Mobility Group Box 1a (HMGB1a) Gene in Red‐Bellied Pacu, Piaractus brachypomus

Carrillo-Godoy

Rondón‐Barragán

2023

Veterinary Medicine International

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Molecular Characterization of Neurogranin (NRGN) Gene from Red‑Bellied Pacu (Piaractus brachypomus)

Rueda-García,

Rondón-Barragán

2023

Mol Neurobiol

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Neurogranin (NRGN) is a small brain protein expressed in various telencephalic areas and plays an essential role in synaptic plasticity by regulating the availability of calmodulin (CaM). The study aims to characterize the neurogranin gene in Colombian native fish, red-bellied pacu, Piaractus brachypomus, its basal tissue expression and differential expression in brain injury and sublethal toxicity by organophosphates. NRGN gene contains an open reading frame of 183 nucleotides encoding for 60 amino acids. Bioinformatics analysis showed an IQ motif necessary in the interaction with CaM. NRGN mRNA was detected in tissues with higher expression in brain, gills, and head kidney. In brain regions, NRGN showed high expression in the telencephalon (TE) and olfactory bulb (OB). In the sublethal toxicity experiment, NRGN mRNA was upregulated in individuals under organophosphate exposure in the OB and optic chiasm (OC). In brain injury experiment, NRGN showed upregulation at 14 days in OC and at 24 h and 7 days in TE. These findings demonstrate the differential expression of NRGN under different experimental conditions which make it a candidate for a biomarker in the brain of P. brachypomus.

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Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus)

Ortiz-Muñoz

Lozano-Villegas

Barragán

2023

Aquaculture and Fisheries

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EVALUACIÓN DE LA TOXICIDAD DEL CLORPIRIFÓS EN ALEVINOS DE CACHAMA BLANCA (Piaractus brachypomus)

Abstract: Toxicity assessement of chlorpyrifos in red-bellied pacu fingerlings (Piaractus brachypomus

Cited by 7 publications

References 68 publications

Molecular Characterization of High Mobility Group Box 1a (HMGB1a) Gene in Red‐Bellied Pacu, Piaractus brachypomus

Molecular Characterization of High Mobility Group Box 1a (HMGB1a) Gene in Red‐Bellied Pacu, Piaractus brachypomus

Molecular Characterization of Neurogranin (NRGN) Gene from Red‑Bellied Pacu (Piaractus brachypomus)

Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu (Piaractus brachypomus)

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