Evaluación de la motilidad espermática a través del sistema C.A.S.A de semen caprino criopreservado bajo diferentes medios diluyentes

Abstract

El estudio evaluó la motilidad espermática y su efecto postdescongelación en semen caprino, en dos medios comerciales (Andromed® y TwoStep®) y diferentes protocolos de congelación (medio completo, con adicción del 10% de yema de huevo, semen centrifugado y sobrenadante seminal), se utilizaron machos de la raza alpina de la Universidad Francisco de Paula Santander Ocaña, el semen fue colectado con electroeyaculador, una vez los medios terminados y parte de los contenidos seminales enteros o centrifugados mezclados, se estabilizó por 2 horas, se envasó en pajillas de 0,5 cc y se congela en vapores de nitrógeno por 10 minutos, las pajillas se llevaron al laboratorio de Andrología de la Universidad del Zulia y por medio del sistema C.A.S.A.(Computer Assisted Sperm Análisis) se evaluaron los parámetros de motilidad como velocidad curvilínea (VCL), velocidad rectilínea (VSL), velocidad lineal (VAP), índice de linealidad (LIN), índice de rectitud (STR), índice de oscilación (ALH), Amplitud media del desplazamiento lateral de la cabeza del espermatozoide (BCF), los datos fueron analizados por medio del procedimiento GLM de SAS versión 9.0; los mejores índices de motilidad (VCL, ALH, BCF) fueron expresados enel tratamiento de contenido seminal centrifugado en medio Andromed®. (p≤0,001))La mejor progresividad espermática (VSL,LIN,STR)se presentó el tratamiento de Semen completo de caprino, criopreservado en medio comercial TwoStep®. ABSTRACT The study evaluated the effect sperm motility and sperm post-thawing in goats, two commercial means (Andromed ® and Two Step ®) and different freezing protocols (complete medium with 10% addition of the egg yolk, semen centrifuged supernatant and seminal ), we used males of the Alpine race of the University Francisco de Paula Santander Ocaña, semen was collected with electroejaculator once finished media and part of the whole and centrifuged seminal contents mixed, stabilized by two hours, packed in 0.5 cc straws and frozen in nitrogen vapor for 10 min, the straws were taken to the laboratory of Andrology at the University of Zulia and through CASA system (Computer Assisted Sperm Analysis) were evaluated motility parameters such as curvilinear velocity (VCL), straight line velocity (VSL), linear velocity (VAP), linearity index (LIN), straightness index (STR) Oscillation Index (ALH ) average amplitude of the lateral displacement of the sperm head (BCF), the data were analyzed by the GLM procedure of SAS version 9.0, the highest rates of motility (VCL, ALH, BCF) were expressed in the treatment of seminal content centrifugation Andromed ® medium. (p ≤ 0.001)) The best progressive sperm (VSL, LIN, STR) will present the full Semen treatment goats, cryopreserved at Two Step ® commercial medium.Keywords: semen, buck, Andromed, Two step.

Increase of the motility of buck semen with Andromed® extender and the addition of HTF

“…Likewise, it is a useful tool in conducting researches where evaluating the semen is necessary or for treatments such as those developed in this study. However, although this study only considered the mass and progressive motility variables, all the sperm cell variables that the CASA system includes must be taken into consideration, including curvilinear velocity, progressive velocity, linear velocity, linearity index, straightness index, oscillation index, and mean amplitude of lateral head displacement (Hernández-Corredor et al, 2013). These variables provide objective parameters for the evaluation of semen diluted in different extenders to facilitate the standardization of the morphometric characteristics of spermatozoa (Vicente-Fiel et al, 2013).…”

“…Diaz et al (2015) also evaluated sperm quality over time (0, 2, 4, 6, and 24 h) with different extenders and found that the best MM is recorded 4 to 24 h after the semen has been refrigerated at 5 °C. Because there is scarce information about the parametric evaluation of buck sperm using the CASA system (Hernández-Corredor et al, 2013), it would be advisable to highlight that this system represents an innovative technology that determines the values of the behavior of spermatozoa stored under fresh, refrigerated, or frozen conditions (Quintero-Moreno et al, 2011). Coupled with an adequate selection of an extender for the conservation of buck semen (Hernández-Corredor et al, 2013), this system is a practical alternative for the conservation of the germplasm that is used in species improvement programs.…”

Sperm Quality Parameters in Mexican Hairless Boars From Yucatan

“…Table 3 shows the results of the descriptive statistics for the motility parameters evaluated using the CASA system. A straightness index (STR) of 68.75% was identified, considered as a slow movement for this parameter according to Hernández et al (2013). Masenya et al (2011) in a comparative study on semen characteristics in Kolbroek (South African indigenous breed) and Large White pigs using the CASA system, obtained the following results for each breed, respectively: VCL (143.3μm/s and 129.1μm/s), VSL (40.9 μm/s and 32.8μm/s), VAP (90.6μm/s and 81.1μm/s), LIN (29.2% and 25.7%), STR (45.8% and 41.2%) and WOB (64.4% and 62.6%), being the VCL and the VAP higher than those obtained in the present work.…”

Bull Semen Obtained on Beef Farms by Electroejaculation: Sperm Quality in the First Two Hours of Storing with Different Extenders and Holding Temperatures

“…In this regard, some authors indicated that an increase in the viscosity of the extender due to the presence of larger molecules in its composition induces this type of curvilinear movement, which occurs naturally in cervical mucus [54]. The viscosity of cervical and oviductal mucus induced hyperactivation of spermatozoa [55,56], and the viscosity of the extender can similarly influence sperm motility [57,58]. This hyperactivity led to a change in motility, increasing VCL and ALH values and decreasing VSL [43,55].…”