Evaluación de Dos Crioprotectores y Tres Curvas de Congelación Programable en la Criopreservación de Semen de Brycon henni (Pisces: Characidae)

Betancur, Giovanni Restrepo; Páez, Juan David Montoya; Chacón, Lucy Arboleda

doi:10.15381/rivep.v28i3.13349

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…(2018) 22% in P. microlepis . Restrepo‐Betancur et al., (2017) 25.9 ± 19.2% in Brycon henni and Atencio‐García et al., (2014) 36.9 ± 9.1% in S. cuspicaudus , percentages similar to those obtained in this study for C. jordani with 2% and 6% EG, but lower in concentrations greater than 10%, 14%, and 16%, with 41.3 ± 1.5, 53.5 ± 1.0 and 40.3 ± 2.1 motility percentages, respectively.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico

et al. 2020

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Genus Chirostoma belongs to Atherinopsidae family and it is an endemic species from the Mesa Central in Mexico. Abundance of its species have decreased and some ones have been placed on the threatened species list, because of overfishing, urbanization, industrialization, destruction, habitat fragmentation, pollution and exotic species introduction. Chirostoma jordani (Woolman, 1894) is a freshwater fish with biological, ecological, cultural, and commercial importance. It has a broad distribution in Lerma drainage, Durango and Mexico City. In this last locality, their populations, although small, still persist in Xochimilco Lake; it is necessary to implement biotechnologies for their conservation, because of these causes and their basic biology. The aim was to standardize a sperm cryopreservation protocol in C. jordani, to determine extender solution, cryoprotective agent type and concentration, equilibrium time, freezing and thawed rate to be applied in assisted reproduction and conservation of genus Chirostoma. Chirostoma jordani adult males were collected in Atlangatepec Dam, Tlaxcala State, Mexico, to fresh seminal evaluation and cryopreservation protocol standardization. Four cryoprotectants effect was evaluated: dimethylsulfoxide (DMSO), methanol (MeOH), ethylene glycol (EG), and glycerol (GL) at five concentrations: 2%, 6%, 10%, 14% and 16% v/v. Higher and lower DMSO and MeOH 10% and EG 14%, decreased post‐thaw motility percentage. Both DMSO and MeOH 10% and EG 14% had the highest post‐thaw motility percentages, 48.8 ± 1.5%, 54.5 ± 1.0% and 53.5 ± 1.0%, at 15, 10, and 5 min equilibrium times, respectively, thawed at 40°C. Chirostoma jordani sperm can be cryopreserved with both DMSO and MeOH 10%, and EG 14%. These ones can be used for assisted reproduction. GL was not efficient, since it presented a post‐thaw motility percentage very low.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico

et al. 2020

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“…Esto podría deberse, en las cualidades propias de cada reproductor y el rol del crioprotector. Siendo el DMSO uno de los mejores crioprotectores por su efectividad en la crioconservación, cuando se trabaja con semen de especies icticas, en su mayoría tropicales (Atencio et al, 2013;Restrepo-Betancur et al, 2017), lo cual se ve reflejado en lo reportado por Varela et al (2012)., quien reporta una tasa de eclosión del 47,3% ±2,5, cuando utilizó 10% de DMSO, en comparación con 37,4% ± 4,5 de eclosión cuando fue con 5% de DMSO, sin embargo, la tasa de eclosión fue inversamente proporcional, a medida que aumentaba la concentración de DMSO, lo cual evidencia el nivel tóxico de este crioprotector intracelular, en mayores proporciones. Esto es un inconveniente por cuanto se requiere obtener mayores tasas de eclosión, pero sin efectos tóxicos secundarios que permitan llegar a niveles comerciales.…”

Section: Colossoma Macropomum "Gamitana"unclassified

EFFECT OF TWO CRYOPRESERVATION TIMES AND TWO CRYOPROTECTANTS, ON THE VIABILITY OF Colossoma macropomum “GAMITANA” SEMEN

Calizaya

2023

rebiol

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The present research work was developed, with the purpose of evaluating the effect of two cryopreservation times and two cryoprotectants, on the viability of Colossoma macropomum semen, "gamitana". and it was developed in the facilities of the “Fernando Alcántara Bocanegra” Research Center, of the Peruvian Amazon Research Institute-IIAP. A sex ratio of 1:2 (Female: Male) was used, collecting semen in a 0.4 M solution of sucrose. For the cryopreservation process, the semen was subjected to two freezing times (6 minutes and 9 minutes) and two levels of cryoprotectants (Level 1:10% DMSO at 0.1 M and level 2:43% dextrose at 0.3 M). It was fertilized with 25 μL of thawed semen, using a factorial design of 22, which were subjected to an ANOVA, at 95% confidence. Fresh semen evaluation shows that motility time presented a significant difference p=0.021. However, in the post-thaw semen evaluation, motility time and motility percentage did not present a significant difference (p > 0.05) for each breeder, but a reduction in the average times was evidenced, compared to fresh semen. For fertilization percentage, only freezing time presented a significant difference (p=0.000001), for male 1 and male 2 no significant differences were found between treatments. For hatching percentage, according to freezing time and percentage of cryoprotectant, for male 1, no significant differences were found p > 0.05 and for male 2, significant differences were found (p=0.043), for cryoprotectant percentage. In relation to larvae malformation percentage, freezing time and type of cryoprotectant, did not show significant differences (p > 0.05), for breeders, however, they were higher in relation to fresh semen treatment. Therefore, it is evident that there would be an unknown factor of male, which did not allow replicating the values obtained, which is manifested in the synergy of variables studied, for this species.

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Cryopreserved Semen Motility of South American Neotropical Fish: A Meta-Analysis

Lassen

Canozzi

Costa

et al. 2021

Reviews in Fisheries Science & Aquaculture

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Evaluación de Dos Crioprotectores y Tres Curvas de Congelación Programable en la Criopreservación de Semen de Brycon henni (Pisces: Characidae)

Cited by 3 publications

References 4 publications

Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico

Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico

EFFECT OF TWO CRYOPRESERVATION TIMES AND TWO CRYOPROTECTANTS, ON THE VIABILITY OF Colossoma macropomum “GAMITANA” SEMEN

Cryopreserved Semen Motility of South American Neotropical Fish: A Meta-Analysis

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