EV71: An emerging infectious disease vaccine target in the Far East?

Xu, Juan; Qian, Yuan; Wang, Shixia; Serrano, Jill M. Grimes; Wei, Li; Huang, Zhibin; Lu, Shan

doi:10.1016/j.vaccine.2010.03.003

Cited by 117 publications

(78 citation statements)

References 64 publications

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“…In this study, African green monkey kidney cells (Vero) were used to study the mechanism of virus because of their relatively high sensitivity of EV71 5. The VLPs of HEV71 virus were expressed and purified through constructing a recombinant baculovirus that coexpresses HEV71 P1 and 3CD proteins by a Bac‐to‐Bac Baculovirus Expression System (Figure S1, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

“…Experimental results verified that HEV71 enters permissive cells via a receptor‐mediated endocytosis. Two receptors for HEV71 have been identified: human P‐selectin glycoprotein ligand‐1 (PSGL‐1) and human scavenger receptor class B member 2 (SCARB2) 3, 5. During this process, the plasma membrane invaginates to form vesicle, which is pivotal for wrapping the virus.…”

Section: Introductionmentioning

confidence: 99%

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The Process of Wrapping Virus Revealed by a Force Tracing Technique and Simulations

Pan

Zhang

et al. 2017

Advanced Science

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Viral entry into the host cell is the first step of virus infection; however, its dynamic process via endocytosis remains largely elusive. Here, the force tracing technique and single particle simulation are combined to investigate the invagination of single human enterovirus 71 (HEV71, a positive single‐stranded RNA virus that is associated with hand, foot, and mouth disease) via cell membranes during its host cell entry. The experimental results reveal that the HEV71 invaginates in membrane vesicles at a force of 58 ± 16 pN, a duration time of 278 ± 68 ms. The simulation further shows that the virus can reach a partially wrapped state very fast, then the upper surface of the virus is covered by the membrane traveling over a long period of time. Combining the experiment with the simulation, the mechanism of membrane wrapping of virus is uncovered, which provides new insights into how the cell is operated to initiate the endocytosis of virus.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Process of Wrapping Virus Revealed by a Force Tracing Technique and Simulations

Pan

Zhang

et al. 2017

Advanced Science

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“…13 The preclinical study showed that NT induced by inactivated EV71 vaccine can protect mice against lethal challenge of EV71 virus. 14,15 Two studies in Taiwan had demonstrated that passive immunization was able to protect against lethal EV71 challenge in new born mice. 1,16 Those results showed that humoral immunity played a key role in protecting against EV71-caused disease.…”

Section: Discussionmentioning

confidence: 99%

Tolerability and immunogenicity of an inactivated enterovirus 71 vaccine in Chinese healthy adults and children

Meng

et al. 2012

Human Vaccines & Immunotherapeutics

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“…The only means of preventing and controlling the spread of EV71 during outbreaks is by public health surveillance. Currently, there is no vaccine available for this viral infection 21 . Several studies have shown that infants of preschool age are at the highest risk of severe EV71 infection 13 , and the transmission of this virus is lower in other age groups 14 .…”

Section: Abstract: Enterovirus 71; Neutralizing Antibodies; Immune Stmentioning

confidence: 99%

“…Several studies have shown that infants of preschool age are at the highest risk of severe EV71 infection 13 , and the transmission of this virus is lower in other age groups 14 . The highly pathogenic potential of EV71 -in addition to its easily transmittable nature -are the causes of a very common pediatric disease 21 . These facts together suggest that public health measures should focus on the population most at risk.…”

Section: Abstract: Enterovirus 71; Neutralizing Antibodies; Immune Stmentioning

confidence: 99%

Evaluation of enterovirus 71 immune status in São Paulo state, Brazil

Luchs

Cilli

Russo

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYAntibodies to Enterovirus 71 (EV71) were evaluated in São Paulo State during [1999][2000][2001][2002][2003][2004][2005]. The titer of neutralizing antibodies against EV71 was determined by microneutralization assay, and a titer of ≥ 1:8 was defined as indicative of protected immunity. Neutralizing antibodies to EV71 were observed in 12.4% (55/442) of sera samples, a low protective rate, suggesting that EV71 infection is uncommon in this region, but that there is a relatively high susceptibility to EV71 related diseases, which is worrying considering the recent Asian outbreaks. Also, a significant location-specific difference in seropositivity was observed. Neutralizing antibodies to EV71 were observed in 8.7% (21/241) of São Paulo metropolitan area sera samples, and 16.9% (34/201) of the sera samples from other municipalities. A high number of Brazilian residents live in country and coastal areas without adequate access to piped water or sanitation. This situation may contribute to the EV71 dissemination in these zones. The analysis of environmental samples could possibly make a valuable contribution to studies on the epidemiology of EV71.

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EV71: An emerging infectious disease vaccine target in the Far East?

Cited by 117 publications

References 64 publications

The Process of Wrapping Virus Revealed by a Force Tracing Technique and Simulations

The Process of Wrapping Virus Revealed by a Force Tracing Technique and Simulations

Tolerability and immunogenicity of an inactivated enterovirus 71 vaccine in Chinese healthy adults and children

Evaluation of enterovirus 71 immune status in São Paulo state, Brazil

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