EUS-guided in vivo microdialysis of the pancreas: a novel technique with potential diagnostic and therapeutic application

Kitano, Masayuki; Sakamoto, Hiroki; Das, Kshaunish; Komaki, Takamitsu; Kudo, Masatoshi

doi:10.1016/j.gie.2009.05.040

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…Moreover, patients suffering from severe AP could be monitored by MD early on provided that MD catheters could be placed in the pancreas using a minimally invasive technique. The placement of specially developed MD probes in the pancreas using transgastric echoendoscopic insertion could be a potential method [16]. The clinical usefulness of MD was recently demonstrated in a study of patients with pancreaticojejunal anastomosis, since the retrieval and analysis of intraperitoneal exudate by MD allowed the early detection of fistula formation [17].…”

Section: Discussionmentioning

confidence: 99%

Microdialysis in Early Detection of Temporary Pancreatic Ischemia in a Porcine Model

et al. 2012

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Background: Ischemic injury to the pancreas occurs in various clinical conditions. A method for online monitoring of pathophysiological events in pancreatic parenchyma is missing. Aims: To assess the timing of microdialysis (MD) technique response on temporary changes in pancreatic perfusion, and to evaluate the relationship between MD data and systemic markers of anaerobic metabolism and inflammation. Methods: In anaesthetized normoventilated pigs, MD probes were placed in right (control) and left (ischemic) pancreatic lobes, respectively. Following the clamping of the vessels, ischemia was verified by tissue oxygen tension (P_tiO₂) measurements. Results: P_tiO₂ decreased within 20 min after the clamping of the vessels, already returning to baseline levels at the first sampling point after the removal of the clamp. MD lactate levels increased, whereas pyruvate and glucose levels decreased at 20 min after the induction of ischemia. These trends continued until the end of ischemia and returned to baseline following reperfusion. Serum lactate, amylase and tumor necrosis factor-alpha levels decreased throughout the protocol time. Conclusion: MD data were in concordance with changes in P_tiO₂, which is indicative of local anaerobic metabolism. MD allowed the detection of pathophysiological processes within the ischemic pancreas at a stage when no elevations of systemic markers of ischemia or inflammation were observed.

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Section: Discussionmentioning

confidence: 99%

Microdialysis in Early Detection of Temporary Pancreatic Ischemia in a Porcine Model

et al. 2012

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“…Brain human [68] brain, blood mouse [31] brain mouse [69] brain (cortex, hippocampus, striatum) rat [12] brain (cortical, subcortical) rhesus monkey [70] Breast human, mouse [71] skin human [72] skin human, ex vivo [73] subcutaneous fat, muscle human [20] skin, soft tissue human [74] muscle human [75] bone, adipose tissue human [19] skeletal muscle, adipose tissue human [76] lung human [77] lung rat [78] kidney, lung, liver rat [79] liver rat [80] heart rabbit [81] pancreas rat [82] peritoneum human [49] heart pig [83] pancreas dog [84] synovial fluid rabbit [85] cerebrospinal fluid rat [33] [23,24]. Tumor drug exposure, a marker linked to clinical outcome, may be reduced dramatically, due to diffusion barriers in solid tumors [25].…”

Section: Organ Species Referencesmentioning

confidence: 99%

Microdialysis Techniques In Pharmacokinetic and Biomarker Studies Past, Present and Future Directions A Review

Erdő¹

2015

Clin Exp Pharmacol

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Microdialysis (MD) techniques were first applied in the early 1960s. The fields of their application comprised probe implantation into the central nervous system (CNS) at the beginning, and then expanded to almost every organ summarized in this article. After its early experimental applications MD became an important tool in the human pharmacokinetic/pharmacodynamic (PK/PD) studies as well. This monitoring technique is capable for investigation of local unbound concentrations of both endogenous and exogenous compounds in the interstitial fluid. The review shows examples for the role of MD in pharmacodynamic studies and for its use in tissue distribution and drug-transporter interaction studies. Determination of test substances in the dialysate samples needs sensitive bioanalytical methods. The main analytical techniques coupled with MD are summarized under the subtitle "Target molecules". New trend in the application of MD is the determination of large molecular entities in the extracellular fluid of target tissues. This approach greatly helps in the discovery of new pathophysiological pathways and identification of new therapeutic intervention strategies for several disorders. Finally, the article gives an overview on the complementary techniques (positron emission tomography, magnetic resonance spectroscopy and open flow microperfusion), and presents their advantages and limitations versus to in vivo MD. In summary, MD techniques have a wide variety of the fields of application. There are several new approaches using this methodology. The relatively low price and the importance of the information gained on the pharmacologically active form of the test articles at the site of interest guarantees a significant position of this technique in the preclinical and clinical research.

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“…However, this is more invasive and presents a difficult challenge for the clinician thus requiring intensive training. A recent approach has been to evaluate the insertion of the microdialysis probe in pancreatic tissue guided by an endoscopic ultrasound device (131), which has the potential to be used in other tissues. In addition, the microdialysis probe has a fragile tip, which can be broken during the probe implantation.…”

Section: Intestinal Microdialysismentioning

confidence: 99%

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Priya¹,

Córcoles²

2012

Sepsis - An Ongoing and Significant Challenge

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EUS-guided in vivo microdialysis of the pancreas: a novel technique with potential diagnostic and therapeutic application

Cited by 8 publications

References 17 publications

Microdialysis in Early Detection of Temporary Pancreatic Ischemia in a Porcine Model

Microdialysis in Early Detection of Temporary Pancreatic Ischemia in a Porcine Model

Microdialysis Techniques In Pharmacokinetic and Biomarker Studies Past, Present and Future Directions A Review

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

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