Europium Nanoparticle-Based High Performing Immunoassay for the Screening of Treponemal Antibodies

Talha, Sheikh M.; Hytönen, Jukka; Westhorpe, Adam; Kumar, Sushil; Khanna, Navin; Pettersson, Kim

doi:10.1371/journal.pone.0084050

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…4 ). Indeed, since acidified condition prevents the growth of ornamentation and spines of foraminifera 28 , 31 , 32 , the lack of ornamentation in smaller Albian species 4 , 8 might reflect adaptation to the acidified oceanic condition. No major extinction of calcareous nannoplankton has been reported during OAE1b.…”

Section: Resultsmentioning

confidence: 99%

Marine Os isotopic evidence for multiple volcanic episodes during Cretaceous Oceanic Anoxic Event 1b

Matsumoto

Kuroda

Coccioni

et al. 2020

Sci Rep

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The Aptian-Albian boundary is marked by one of the major oceanic perturbations during the Cretaceous, called Oceanic Anoxic Event (OAE) 1b. Extensive volcanic episodes at the Southern Kerguelen Plateau has been suggested as the trigger of OAE1b, but compelling evidence remains lacking. Here, we reconstructed the temporal variations of marine Os isotopic ratios across the Aptian-Albian boundary in the Tethyan and Pacific pelagic sedimentary records to elucidate the causal links between OAE1b, the biotic turnover, and volcanic episodes. Our new Os isotopic records show two negative spikes that correlate with a period of planktonic foraminiferal turnover across the Aptian-Albian boundary during OAE1b and suggest multiple submarine volcanic events. By comparing our Os isotopic profile with carbon isotopic compositions of carbonate, CaCO 3 content, and the relative abundances of agglutinated foraminifera, we conclude that ocean acidification caused by the massive release of co 2 through extensive volcanic episodes could have promoted the major planktonic foraminiferal turnover during OAE1b. The mid-Cretaceous (Barremian-Turonian) is punctuated by repeated Oceanic Anoxic Events (OAEs), which represent intervals of global and episodic burial of organic-rich sediments on the seafloor under oxygen-deficient bottom waters. Several organic-rich sedimentary deposits are recorded in the uppermost Aptian to the Lower Albian, mainly in the Tethys and Atlantic Oceans 1-5 (Fig. 1). In particular, four prominent black shale horizons (113/Jacob, Kilian, Urbino/Paquier, and Leenhardt equivalent levels) have been identified as the sedimentary expression of OAE1b 4. OAE1b is characterized by (1) an exceptionally long duration (~ 3.8 Myr) with intermittent occurrences of oxygen-depleted bottom water conditions, (2) a global carbon-cycle perturbation, and (3) a major marine biotic turnover 4. In particular, planktonic foraminifera experienced one of the most significant turnovers in their evolutionary history, where large, heavily calcified planktonic foraminifera of the Aptian were replaced by small, weakly calcified taxa characteristic of the Albian 4,6-8. Since 40 Ar-39 Ar ages of the Southern Kerguelen Plateau basalt (Fig. 1) (109.2-119.0 Ma) 9 roughly correspond to the duration of OAE1b (~ 110.5-114.5 Ma), volcanic episodes associated with the break-up of Gondwana have been suggested as the trigger of OAE1b 1,3,5. However, the issue remains debated because of the large chronological uncertainties of the 40 Ar-39 Ar ages of the basaltic rocks and the ages of the sedimentary sequences. Marine osmium (Os) isotopic records (187 Os/ 188 Os) reflect the balance between the continental Os flux (187 Os/ 188 Os ≈ 1.0-1.5) and mantle/hydrothermal and extraterrestrial Os fluxes (187 Os/ 188 Os ≈ 0.12-0.13) to the global ocean 10. Thus, 187 Os/ 188 Os values of palaeo-seawater preserved in sedimentary rocks represent a robust proxy to constrain the timing of massive input of unradiogenic Os through submarine hydrothermal or volcanic eruption. ...

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Section: Resultsmentioning

confidence: 99%

Marine Os isotopic evidence for multiple volcanic episodes during Cretaceous Oceanic Anoxic Event 1b

Matsumoto

Kuroda

Coccioni

et al. 2020

Sci Rep

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“…Lanthanide-doped polymer particles, silica nanoparticles, and fluoride nanoparticles with long luminescence lifetimes are also in development. − However, they also need high-energy UV light for excitation. Specifically, for lanthanide-doped fluoride nanoparticles, although they can be excited by near-infrared (NIR) light through upconversion for bioapplications, NIR excitation of these particles still requires high-power lasers and costly laser drivers. , These fluoride nanoparticles also suffer from low brightness or low QYs at 0.1–5%, which necessitates the need for more sensitive but expensive CCD cameras. , …”

Section: Current Time-resolved Luminescence Nanoprobes and Their Limi...mentioning

confidence: 99%

Review of Mn-Doped Semiconductor Nanocrystals for Time-Resolved Luminescence Biosensing/Imaging

Sreenan

Lee

Wan

et al. 2022

ACS Appl. Nano Mater.

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Colloidal semiconductor nanocrystals (NCs) have been developed for decades and are widely applied in biosensing/imaging. However, their biosensing/imaging applications are mainly based on luminescence-intensity measurement, which suffers from autofluorescence in complex biological samples and thus limits the biosensing/imaging sensitivities. It is expected for these NCs to be further developed to gain luminescence features that can overcome sample autofluorescence. On the other hand, time-resolved luminescence measurement utilizing long-lived-luminescence probes is an efficient technique to eliminate short-lived autofluorescence of samples while recording time-resolved luminescence of the probes for signal measurement after pulsed excitation from a light source. Despite time-resolved measurement being very sensitive, the optical limitations of many of the current long-lived-luminescence probes cause time-resolved measurement to be generally performed in laboratories with bulky and costly instruments. In order to apply highly sensitive time-resolved measurement for in-field or point-of-care (POC) testing, it is essential to develop probes possessing high brightness, low-energy (visible-light) excitation, and long lifetimes of up to milliseconds. Such desired optical features can significantly simplify the design criteria of time-resolved measurement instruments and facilitate the development of low-cost, compact, sensitive instruments for in-field or POC testing. Mn-doped NCs have recently been in rapid development and provide a strategy to solve the challenges faced by both colloidal semiconductor NCs and time-resolved luminescence measurement. In this review, we outline the major achievements in the development of Mn-doped binary and multinary NCs, with emphasis on their synthesis approaches and luminescence mechanisms. Specifically, we demonstrate how researchers approached these obstacles to achieve the aforementioned desired optical properties on the basis of the progressive understanding of Mn emission mechanisms. Afterward, we review representative applications of Mn-doped NCs in time-resolved luminescence biosensing/imaging and present the potential of Mn-doped NCs in advancing time-resolved luminescence biosensing/imaging for in-field or POC testing.

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“…Such short lifetimes of transition-metal-complexes require high-speed electronics for signal recording and thus increase the instrument complexity and cost [ 3 ]. Besides luminescent metal-complex probes, lanthanide-doped polymer particles, with both silica nanoparticles and fluoride nanoparticles, with long fluorescence lifetimes are also in development [ 8 , 9 , 10 , 11 ]. Lanthanide-doped polymer particles with silica nanoparticles still need high energy for excitation as lanthanide complexes.…”

Section: Introductionmentioning

confidence: 99%

Optical Properties of Mn-Doped CuGa(In)S-ZnS Nanocrystals (NCs): Effects of Host NC and Mn Concentration

2022

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Time-gated fluorescence measurement (TGFM) using long-life fluorescence probes is a highly sensitive fluorescence-measurement technology due to the inherently high signal-to-background ratio. Although many probes for TGFM such as luminescent-metal-complex probes and lanthanide-doped nanoparticles are in development, they generally need sophisticated/expensive instruments for biosensing/imaging applications. Probes possessing high brightness, low-energy (visible light) excitation, and long lifetimes up to milliseconds of luminescence, are highly desired in order to simplify the optical and electronic design of time-gated instruments (e.g., adopting non-UV-grade optics or low-speed electronics), lower the instrument complexity and cost, and facilitate broader applications of TGFM. In this work, we developed Mn-doped CuGa(In)S-ZnS nanocrystals (NCs) using simple and standard synthetic steps to achieve all the desired optical features in order to investigate how the optical properties (fluorescence/absorption spectra, brightness, and lifetimes) of the Mn-doped NCs are affected by different host NCs and Mn concentrations in host NCs. With optimal synthetic conditions, a library of Mn-doped NCs was achieved that possessed high brightness (up to 47% quantum yield), low-energy excitation (by 405 nm visible light), and long lifetimes (up to 3.67 ms). Additionally, the time-domain fluorescence characteristics of optimal Mn-doped NCs were measured under pulsed 405 nm laser excitation and bandpass-filter-based emission collection. The measurement results indicate the feasibility of these optimal Mn-doped NCs in TGFM-based biosensing/imaging.

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Europium Nanoparticle-Based High Performing Immunoassay for the Screening of Treponemal Antibodies

Cited by 5 publications

References 24 publications

Marine Os isotopic evidence for multiple volcanic episodes during Cretaceous Oceanic Anoxic Event 1b

Marine Os isotopic evidence for multiple volcanic episodes during Cretaceous Oceanic Anoxic Event 1b

Review of Mn-Doped Semiconductor Nanocrystals for Time-Resolved Luminescence Biosensing/Imaging

Optical Properties of Mn-Doped CuGa(In)S-ZnS Nanocrystals (NCs): Effects of Host NC and Mn Concentration

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