Europium (III) chelate nanoparticle-based lateral flow immunoassay strips for rapid and quantitative detection of cystatin C in serum

Bian, Lun; Xiong, Yufeng; Zhao, Hui; Guo, Haipeng; Li, Zhaoyue; Yao, Ke; Zhang, Zhigao; Liu, Tiancai; Wu, Yixuan; Lin, Guanfeng

doi:10.1016/j.jchromb.2022.123133

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…Another important conjugation protocol of europium nanoparticles is described by Bian et al (2022) . Europium-chelate nanoparticles (EU-CNs) (1 mg) were resuspended in 500 µl solution consisting of 25 mmol/L MES (pH 6.1) and 1.25 mmol/L EDC and 10 mmol/L sulfo-NHS with shaking for 30 min.…”

Section: Conjugation Protocolsmentioning

confidence: 99%

“…The pellets were resuspended in 400 µl 25 mmol/L PBS buffer (pH 7) and the antibody solution (25 μg of antiCys C labeling McAb or RIgG dissolved in 100 μl of binding buffer) was added and mixed for 2 h. The sites left free after the conjugation process were blocked by the addition of 500 μl BSA solution (5%). The conjugate was resuspended in 100 μl of labeling antibody storage buffer (25 mmol/L Tris, 0.9% NaCl (w/v), 1% sucrose (w/v), 1% Trehalose (w/v), 5% BSA (w/v), 0.05% Proclin-300 (v/v) and 0.05% TWEEN-20 (v/v), pH 7.2) ( Bian et al, 2022 ).…”

Section: Conjugation Protocolsmentioning

confidence: 99%

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Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Mirica

Stan²,

Chelcea³

et al. 2022

Front. Bioeng. Biotechnol.

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LFIA is one of the most successful analytical methods for various target molecules detection. As a recent example, LFIA tests have played an important role in mitigating the effects of the global pandemic with SARS-COV-2, due to their ability to rapidly detect infected individuals and stop further spreading of the virus. For this reason, researchers around the world have done tremendous efforts to improve their sensibility and specificity. The development of LFIA has many sensitive steps, but some of the most important ones are choosing the proper labeling probes, the functionalization method and the conjugation process. There are a series of labeling probes described in the specialized literature, such as gold nanoparticles (GNP), latex particles (LP), magnetic nanoparticles (MNP), quantum dots (QDs) and more recently carbon, silica and europium nanoparticles. The current review aims to present some of the most recent and promising methods for the functionalization of the labeling probes and the conjugation with biomolecules, such as antibodies and antigens. The last chapter is dedicated to a selection of conjugation protocols, applicable to various types of nanoparticles (GNPs, QDs, magnetic nanoparticles, carbon nanoparticles, silica and europium nanoparticles).

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Section: Conjugation Protocolsmentioning

confidence: 99%

Section: Conjugation Protocolsmentioning

confidence: 99%