Europium (III) chelate microparticle-based lateral flow immunoassay strips for rapid and quantitative detection of antibody to hepatitis B core antigen

Liang, Rong-Liang; Deng, Qiaoting; Chen, Zhenhua; Xu, Xuping; Zhou, Jianwei; Liang, Junyu; Dong, Zhi-Ning; Liu, Tiancai; Wu, Yixuan

doi:10.1038/s41598-017-14427-4

Cited by 28 publications

(16 citation statements)

References 30 publications

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“…NGS is used to sequence genomes, DNAs and RNAs, and can be used to differentiate host genetic information from viral sequences. This, therefore, provides a powerful tool to diagnose viruses, and would be particularly useful in scenarios where patients present with a disease and the causative-pathogen is unidentified (Barzon et al, Hu et al (2017), Liang et al (2017), Wiriyachaiporn et al (2017), Kumar et al (2018), Rong et al (2019) 2013). While molecular, immuno-and cell-culture methods can be used to identifying unknown pathogens in an investigational "process of elimination" approach, NGS offers a more timeeffective method to achieve the same output (Adams et al, 2009).…”

Section: Future Directionsmentioning

confidence: 99%

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Cassedy

Parle‐McDermott

O’Kennedy

2021

Front. Mol. Biosci.

118

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Viruses are ubiquitous in the environment. While many impart no deleterious effects on their hosts, several are major pathogens. This risk of pathogenicity, alongside the fact that many viruses can rapidly mutate highlights the need for suitable, rapid diagnostic measures. This review provides a critical analysis of widely used methods and examines their advantages and limitations. Currently, nucleic-acid detection and immunoassay methods are among the most popular means for quickly identifying viral infection directly from source. Nucleic acid-based detection generally offers high sensitivity, but can be time-consuming, costly, and require trained staff. The use of isothermal-based amplification systems for detection could aid in the reduction of results turnaround and equipment-associated costs, making them appealing for point-of-use applications, or when high volume/fast turnaround testing is required. Alternatively, immunoassays offer robustness and reduced costs. Furthermore, some immunoassay formats, such as those using lateral-flow technology, can generate results very rapidly. However, immunoassays typically cannot achieve comparable sensitivity to nucleic acid-based detection methods. Alongside these methods, the application of next-generation sequencing can provide highly specific results. In addition, the ability to sequence large numbers of viral genomes would provide researchers with enhanced information and assist in tracing infections.

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Section: Future Directionsmentioning

confidence: 99%

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Cassedy

Parle‐McDermott

O’Kennedy

2021

Front. Mol. Biosci.

118

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“…In addition to avian influenza, , Eu 3+ chelate-embedded polymeric nanoparticles have been employed for the detection of E. coli, hepatitis B, and procalcitonin, a host marker for bacterial infections. Several commercial platforms based on these particles have been developed as well, including the Sofia Influenza A+B tests (Quidel, Inc.) − and the aQcare Chlamydia TRF test (Medisensor, Inc.), , which have been extensively evaluated in the literature.…”

Section: Metal-based Signal Generationmentioning

confidence: 99%

Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics

et al. 2018

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Infectious diseases claim millions of lives each year. Robust and accurate diagnostics are essential tools for identifying those who are at risk and in need of treatment in low-resource settings. Inorganic complexes and metal-based nanomaterials continue to drive the development of diagnostic platforms and strategies that enable infectious disease detection in low-resource settings. In this review, we highlight works from the past 20 years in which inorganic chemistry and nanotechnology were implemented in each of the core components that make up a diagnostic test. First, we present how inorganic biomarkers and their properties are leveraged for infectious disease detection. In the following section, we detail metal-based technologies that have been employed for sample preparation and biomarker isolation from sample matrices. We then describe how inorganic- and nanomaterial-based probes have been utilized in point-of-care diagnostics for signal generation. The following section discusses instrumentation for signal readout in resource-limited settings. Next, we highlight the detection of nucleic acids at the point of care as an emerging application of inorganic chemistry. Lastly, we consider the challenges that remain for translation of the aforementioned diagnostic platforms to low-resource settings.

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“…Moreover, colloidal gold nanoparticles as labels on ICS have been used to detect T. spiralis infection [ 23 , 24 ]. Recently, EuNPs, which are time-resolved fluorescent microspheres with the advantages of a large Stokes shift, a narrow emission spectrum and a long quenching time, have begun to replace colloidal gold as the labels used in mainstream ICS methods for clinical disease detection, such as hepatitis B virus and porcine epidemic diarrhea virus [ 25 , 26 ]. For investigating the seroconversion of infected pigs, a novel EuNPs-ICS detection method based on ML-ES antigens and PAW-ES antigens was developed for T. spiralis infection.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs

Wang

Tang

Zhao

et al. 2021

Vet Res

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Trichinellosis, which is caused by nematodes of the genus Trichinella, is one of the most important zoonotic parasite diseases in the world. A rapid and sensitive immunochromatographic strip (ICS) based on Eu (III) nanoparticles (EuNPs) was developed for the detection of Trichinella spiralis (T. spiralis) infection in pigs. T. spiralis muscle larvae excretory secretory or preadult worm excretory secretory (ML-ES or PAW-ES) antigens were conjugated with EuNPs probes to capture T. spiralis-specific antibodies in pig sera, after which the complex bound to mouse anti-pig IgG deposited on the test line (T-line), producing a fluorescent signal. In the pigs infected with 100, 1000 and 10 000 ML, seroconversion was first detectable for the EuNPs-ML-ES ICS at 30, 25 and 21 days post-infection (dpi) and for the EuNPs-PAW-ES ICS at 25, 21 and 17 dpi. These results show that EuNPs-PAW-ES ICS detects anti-Trichinella IgG in pigs 4–5 days earlier that test using ML-ES antigens. Our ICS have no cross reaction with other parasite infection sera. Furthermore, the detection process could be completed in 10 min. This study indicated that our ICS can be used for the detection of the circulating antibodies in early T. spiralis infection and provide a novel method for on-site detection of T. spiralis infection in pigs.

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Europium (III) chelate microparticle-based lateral flow immunoassay strips for rapid and quantitative detection of antibody to hepatitis B core antigen

Cited by 28 publications

References 30 publications

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics

Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs

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