European minnows through time: museum collections aid genetic assessment of species introductions in freshwater fishes (Cyprinidae: Phoxinus species complex)

Palandačić, Anja; Kruckenhauser, Luise; Ahnelt, Harald; Mikschi, Ernst

doi:10.1038/s41437-019-0292-1

Cited by 35 publications

(105 citation statements)

References 77 publications

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“…Whilst the three species have minimal morphological differences (Kottelat and Freyhof 2007), they formed independent and well-supported groups that were also in agreement with recent phylogenetic studies (Palandačić et al 2017;Corral-Lou et al 2019). P. lumaireul Schinz (1840) (subclade 1a sensu Palandačić et al 2017) has been revalidated as an endemic to the north Adriatic basin (Kottelat and Freyhof 2007;Palandačić et al 2017Palandačić et al , 2020.…”

Section: Discussionsupporting

confidence: 83%

“…The RAG 1 results revealed only a few of the least frequent haplotypes that were separated from the main haplotype by several mutational steps (four to five), could possibly belong to other species than the native P. lumaireul. The utility of RAG 1 marker was limited in depicting the phylogenetic relations between minnows that produced an uninformative network where well-defined mitochondrial lineages were not clear, as was evident in other studies (Corral-Lou et al 2019;Palandačić et al 2020). Although this could be due to the tendency of this species to hybridize (Corral-Lou et al 2019), issues including incomplete lineage sorting and artefacts resulting from the gametic phasing or sequencing processes cannot be excluded from the evaluation of these results (Palandačić et al 2020).…”

Section: Discussionmentioning

confidence: 96%

“…Phoxinus csikii (subclade 5a sensu Palandačić et al 2017) was first described by Hankó 1922 from a karstic brook located in Montenegro and has been recently revalidated, based on both molecular (Palandačić et al 2017) and morphological evidence (Ramler et al 2016). Its natural distribution is the lower and middle part of the Danube River watershed of the Balkan Peninsula (Palandačić et al 2020). P. septimaniae was originally described by Kottelat (2007) as native to the French Mediterranean basins (south-eastern France); it has been also detected in river basins in north-eastern Spain (Corral-Lou et al 2019;Garcia-Raventós et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Although currently confined to the Alpine lakes, there is an inherent risk that the alien P. csikii and P. septimaniae could be unintentionally dispersed into streams in the Po basin by anthropogenic translocations (e.g. illegal stocking, Fernández et al 2019), as has occurred in Germany (Knebelsberger et al 2015), Austria and Croatia (Palandačić et al 2020). If this happens, it is then probable that they would colonise streams where only endemic P. lumaireul is currently present.…”

Section: Discussionmentioning

confidence: 99%

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Species composition of introduced and natural minnow populations of the Phoxinus cryptic complex in the westernmost part of the Po River Basin (north Italy)

Santis

Delmastro²,

Vanetti

et al. 2020

Biol Invasions

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Invasive alien species are a major driver of biodiversity loss, with their impacts potentially more intense when complexes of cryptic species are involved. In freshwaters, the anthropogenic manipulation of fish communities has resulted in altered fish communities, and in Europe has increased the complexity of Phoxinus species assemblages. Here, we investigated the Phoxinus communities of the westernmost part of the Po river basin, where adjacent freshwater ecosystems (Alpine high-altitude lakes and lowland streams) are representative of different management strategies (i.e. manipulated fish communities via stocking in Alpine lakes vs. natural populations in streams). We tested the genetic composition of the cryptic Phoxinus populations inhabiting these waters, as the species are morphologically indistinct. Sequences of the mitochondrial cytochrome oxidase I (COI) were obtained from 239 specimens, with the results indicating that 17 Alpine high-altitude lakes are now populated by a complex of Phoxinus species, comprising P. septimaniae (native to the Mediterranean area of France), P. csikii (native to the Central Balkans) and P. lumaireul (native to the North Adriatic Sea basins). Their introduction resulted from their use as angling live baits. Minnow populations in lowland streams were primarily comprised of native P. lumaireul, with only a single P. csikii specimen detected. While nuclear sequences of the recombination activating gene 1 (RAG1) marker were not useful for tracking the presence of alien alleles in these stream populations, the COI data emphasised the importance of using molecular tools to investigate cryptic species complexes that have been modified by anthropogenic activities.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Species composition of introduced and natural minnow populations of the Phoxinus cryptic complex in the westernmost part of the Po River Basin (north Italy)

Santis

Delmastro²,

Vanetti

et al. 2020

Biol Invasions

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“…csikii (BOLD:AAC8036) and P . septimaniae (BOLD:AAY8765, based on Palandačić et al [ 48 , 49 ]. In general, the assignment to multiple BINs indicates the presence of regional genetic variants or even cryptic, unrecognized species [ 19 , 50 – 52 ].…”

Section: Discussionmentioning

confidence: 99%

Adding DNA barcoding to stream monitoring protocols – What’s the additional value and congruence between morphological and molecular identification approaches?

2021

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Although aquatic macroinvertebrates and freshwater fishes are important indicators for freshwater quality assessments, the morphological identification to species-level is often impossible and thus especially in many invertebrate taxa not mandatory during Water Framework Directive monitoring, a pragmatism that potentially leads to information loss. Here, we focus on the freshwater fauna of the River Sieg (Germany) to test congruence and additional value in taxa detection and taxonomic resolution of DNA barcoding vs. morphology-based identification in monitoring routines. Prior generated morphological identifications of juvenile fishes and aquatic macroinvertebrates were directly compared to species assignments using the identification engine of the Barcode of Life Data System. In 18% of the invertebrates morphology allowed only assignments to higher systematic entities, but DNA barcoding lead to species-level assignment. Dissimilarities between the two approaches occurred in 7% of the invertebrates and in 1% of the fishes. The 18 fish species were assigned to 20 molecular barcode index numbers, the 104 aquatic invertebrate taxa to 113 molecular entities. Although the cost-benefit analysis of both methods showed that DNA barcoding is still more expensive (5.30–8.60€ per sample) and time consuming (12.5h), the results emphasize the potential to increase taxonomic resolution and gain a more complete profile of biodiversity, especially in invertebrates. The provided reference DNA barcodes help building the foundation for metabarcoding approaches, which provide faster sample processing and more cost-efficient ecological status determination.

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The minnow Phoxinus lumaireul (Leuciscidae) shifts the Adriatic–Black Sea basin divide in the north‐western Dinaric Karst region

et al. 2022

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Karst landscapes are characterized by intermittent and sinking streams. The most common method used to study underground hydrological connections in karst is tracing tests. However, a more biologically oriented approach has been suggested: analysis of the genetic structure of aquatic organisms. Biological tracers can be sought among trogloxenes, that is, surface species that occasionally enter caves and groundwater. One such example is the fish genus Phoxinus, which exhibits high genetic diversity and complex phylogeography in the Balkan Peninsula. In the north‐western Dinaric Karst, the complex hydrological network was digitalized in 2020. Contemporaneously, Phoxinus lumaireul populations in the Slovenian Dinaric Karst were intensively sampled and analysed for fragments of two mitochondrial genes and one nuclear gene. The derived phylogeographic structure and data on hydrological connections were compared to evaluate support for three alternative scenarios: The genetic structure (1) is a consequence of the ongoing geneflow through underground connections, (2) reflects a previous hydrological network or (3) is an outcome of anthropogenic translocations. The results suggest that the first two scenarios seem to have played a major role, while the third has not had profound effects on the genetic composition. Comparison between the genetic structure of Slovenian Dinaric Karst sampling sites and that of hydrologically isolated reference sampling sites indicated a greater genetic connectivity in the former. Moreover, the range of Adriatic (1a) and Black Sea (1c) haplotypes does not correspond to the Adriatic–Black Sea basin divide but is shifted northwards.

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European minnows through time: museum collections aid genetic assessment of species introductions in freshwater fishes (Cyprinidae: Phoxinus species complex)

Cited by 35 publications

References 77 publications

Species composition of introduced and natural minnow populations of the Phoxinus cryptic complex in the westernmost part of the Po River Basin (north Italy)

Species composition of introduced and natural minnow populations of the Phoxinus cryptic complex in the westernmost part of the Po River Basin (north Italy)

Adding DNA barcoding to stream monitoring protocols – What’s the additional value and congruence between morphological and molecular identification approaches?

The minnow Phoxinus lumaireul (Leuciscidae) shifts the Adriatic–Black Sea basin divide in the north‐western Dinaric Karst region

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