European efforts to harmonize typing of meningococci

Vogel, Ulrich

doi:10.1016/j.ijmm.2011.09.001

Cited by 4 publications

(5 citation statements)

References 33 publications

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“… 33 Multilocus sequence typing (MLST) is a molecular technique that has been developed and has been increasingly used for MenB typing. 34 MLST is performed by sequencing of selected genes encoding housekeeping enzymes. The loci of each house-keeping gene define the allelic profile or so-called sequence type (ST).…”

Section: Discussionmentioning

confidence: 99%

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Routine vaccination against MenB

Kaaijk

Ende

Luytjes

2013

Human Vaccines & Immunotherapeutics

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Effective polysaccharide(conjugate) vaccines against Neisseria meningitidis serogroups A, C, W, and Y have been widely used, but serogroup B meningococci remain a major cause of severe invasive meningococcal disease (IMD) worldwide, especially in infants. Recently, a vaccine, 4CMenB (Bexsero®), containing three recombinant proteins, and outer membrane vesicles (OMV) derived from a serogroup B meningococcal strain (MenB) has been licensed in Europe and Australia and is indicated for persons aged 2 mo or older. This article discusses what should be considered to enable a successful implementation of a broad coverage MenB vaccine in national immunization programs. Epidemiology data, vaccine characteristics including vaccine coverage, immunogenicity, post-implementation surveillance and costs are relevant aspects that should be taken into account when selecting an appropriate immunization strategy. The potential impact on strain variation and carriage, as well as monitoring vaccine effectiveness, and rare but potentially serious adverse events are points that need to be included in a post-implementation surveillance plan.

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Section: Discussionmentioning

confidence: 99%

“…The combination of antigen sequence typing of PorA and FetA together with MLST seems to provide a robust framework, which can be complemented by sequence typing of other antigens and measurement of their expression. 34 …”

Section: Discussionmentioning

confidence: 99%

Routine vaccination against MenB

Kaaijk

Ende

Luytjes

2013

Human Vaccines & Immunotherapeutics

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“…Vaccine antigen typing especially needs to be flexible due to various approaches to meningococcus serogroup B subcapsular antigen vaccine development (42,48). In Europe, for logistic and financial reasons, many national reference laboratories seem to be overburdened by the effort to fulfill the typing requirements of the European Centre for Disease Control and Prevention (ECDC), which include serogroup, PorA and FetA types, and the sequence type (16,47). Running of seven PCRs and 14 sequencing reactions for a complete MLST scheme, despite all possibilities of automation, is an obvious challenge if done on hundreds of isolates.…”

Section: Discussionmentioning

confidence: 99%

“…Many countries therefore established reference laboratories for laboratory surveillance of disease, in addition to statutory notification. Typing of the organism is a major task of reference laboratories (47). DNA sequence-based typing has replaced earlier immunotyping (46).…”

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confidence: 99%

Ion Torrent Personal Genome Machine Sequencing for Genomic Typing of Neisseria meningitidis for Rapid Determination of Multiple Layers of Typing Information

et al. 2012

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Neisseria meningitidis causes invasive meningococcal disease in infants, toddlers, and adolescents worldwide. DNA sequence-based typing, including multilocus sequence typing, analysis of genetic determinants of antibiotic resistance, and sequence typing of vaccine antigens, has become the standard for molecular epidemiology of the organism. However, PCR of multiple targets and consecutive Sanger sequencing provide logistic constraints to reference laboratories. Taking advantage of the recent development of benchtop next-generation sequencers (NGSs) and of BIGSdb, a database accommodating and analyzing genome sequence data, we therefore explored the feasibility and accuracy of Ion Torrent Personal Genome Machine (PGM) sequencing for genomic typing of meningococci. Three strains from a previous meningococcus serogroup B community outbreak were selected to compare conventional typing results with data generated by semiconductor chip-based sequencing. In addition, sequencing of the meningococcal type strain MC58 provided information about the general performance of the technology. The PGM technology generated sequence information for all target genes addressed. The results were 100% concordant with conventional typing results, with no further editing being necessary. In addition, the amount of typing information, i.e., nucleotides and target genes analyzed, could be substantially increased by the combined use of genome sequencing and BIGSdb compared to conventional methods. In the near future, affordable and fast benchtop NGS machines like the PGM might enable reference laboratories to switch to genomic typing on a routine basis. This will reduce workloads and rapidly provide information for laboratory surveillance, outbreak investigation, assessment of vaccine preventability, and antibiotic resistance gene monitoring.

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“…In addition, there are some molecular tests available to discriminate between different bacterial species or serogroups; e.g., N. meningitidis serogroups B, C, Y and W135 [27,28,29,30], S. pneumoniae [11] and H. influenzae [31]. Serogrouping of most meningitis-associated strains is conducted using a range of methods e.g., culture, serology and immunological tests at reference laboratories [14] and more latterly sequencing [32], however there is still a need for harmonization of methodologies between laboratories [33].…”

Section: Introductionmentioning

confidence: 99%

Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species

et al. 2018

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Meningitis is commonly caused by infection with a variety of bacterial or viral pathogens. Acute bacterial meningitis (ABM) can cause severe disease, which can progress rapidly to a critical life-threatening condition. Rapid diagnosis of ABM is critical, as this is most commonly associated with severe sequelae with associated high mortality and morbidity rates compared to viral meningitis, which is less severe and self-limiting. We have designed a microarray for detection and diagnosis of ABM. This has been validated using randomly amplified DNA targets (RADT), comparing buffers with or without formamide, in glass slide format or on the Alere ArrayTubeTM (Alere Technologies GmbH) microarray platform. Pathogen-specific signals were observed using purified bacterial nucleic acids and to a lesser extent using patient cerebral spinal fluid (CSF) samples, with some technical issues observed using RADT and glass slides. Repurposing the array onto the Alere ArrayTubeTM platform and using a targeted amplification system increased specific and reduced nonspecific hybridization signals using both pathogen nucleic and patient CSF DNA targets, better revealing pathogen-specific signals although sensitivity was still reduced in the latter. This diagnostic microarray is useful as a laboratory diagnostic tool for species and strain designation for ABM, rather than for primary diagnosis.

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European efforts to harmonize typing of meningococci

Cited by 4 publications

References 33 publications

Routine vaccination against MenB

Routine vaccination against MenB

Ion Torrent Personal Genome Machine Sequencing for Genomic Typing of Neisseria meningitidis for Rapid Determination of Multiple Layers of Typing Information

Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species

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